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129 条记录 (耗时 0.045 秒)

    Particles for diagnostic and therapeutic use
    1.
    发明申请
    Particles for diagnostic and therapeutic use 有权
    用于诊断和治疗用途的颗粒

    公开(公告)号：US20060121506A1

    公开(公告)日：2006-06-08

    申请号：US11273275

    申请日：2005-11-14

    申请人： Sharat Singh ,  John Pease ,  Jacqueline Sadakian ,  Daniel Wagner ,  Edwin Ullman

    发明人： Sharat Singh ,  John Pease ,  Jacqueline Sadakian ,  Daniel Wagner ,  Edwin Ullman

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12M1/34

    CPC分类号： G01N33/587 ,  C12Q1/6816 ,  Y10T436/10 ,  C12Q2563/155 ,  C12Q2523/319

    摘要： Methods, compositions and kits are disclosed. The compositions are light emitting and comprise a polymeric matrix having dissolved therein a photoactive compound. The composition has the characteristic that, after activation of the photoactive compound, the rate of decrease in the intensity of light emission at any time during a 20-fold decrease in the intensity is proportional to the intensity of the light emission. In one embodiment the polymeric matrix is comprised of particles of about 20 nm to about 100 μm in diameter to which is bound a specific binding pair member. The particles generally comprise a polymeric matrix having dissolved therein about 1 to about 20% by weight of a dopant. The compositions may be used in methods for determining an analyte. A combination is provided comprising (1) a medium suspected of containing the analyte, (2) and the aforementioned composition. The photoactive substance is activated and the effect of the activating on the optical properties of the combination is detected. The presence and amount of the effect is related to the presence and amount of the analyte in the medium. Also disclosed are kits for use in an assay.

    摘要翻译： 公开了方法，组合物和试剂盒。 组合物是发光的并且包含其中溶解有光敏化合物的聚合物基质。 该组合物具有以下特征：在光活性化合物活化后，强度降低20倍的任何时间的发光强度的降低率与发光强度成比例。 在一个实施方案中，聚合物基质包括直径约20nm至约100μm的颗粒，其与特定结合对成员结合。 颗粒通常包含其中溶解约1至约20重量％的掺杂剂的聚合物基质。 该组合物可用于测定分析物的方法中。 提供的组合包括（1）怀疑含有分析物的介质，（2）和上述组合物。 光活性物质被激活，并且检测到激活对组合的光学性质的影响。 影响的存在和数量与介质中分析物的存在和数量有关。 还公开了用于测定的试剂盒。

    Assays for the detection of anti-TNF drugs and autoantibodies
    4.
    发明授权
    Assays for the detection of anti-TNF drugs and autoantibodies 有权
    用于检测抗TNF药物和自身抗体的测定

    公开(公告)号：US08574855B2

    公开(公告)日：2013-11-05

    申请号：US13441727

    申请日：2012-04-06

    申请人： Sharat Singh ,  Shui Long Wang ,  Linda Ohrmund

    发明人： Sharat Singh ,  Shui Long Wang ,  Linda Ohrmund

    IPC分类号： G01N33/53

    CPC分类号： G01N33/564 ,  G01N33/5091 ,  G01N33/6854 ,  G01N2800/52

    摘要： The present invention provides assays for detecting and measuring the presence or level of anti-TNFα drug therapeutics and autoantibodies in a sample. The present invention is useful for optimizing therapy and monitoring patients receiving anti-TNFα drug therapeutics to detect the presence or level of autoantibodies (e.g., HACA and/or HAHA) against the drug.

    摘要翻译： 本发明提供用于检测和测量样品中抗TNFα药物治疗剂和自身抗体的存在或水平的测定。 本发明可用于优化治疗和监测接受抗TNFα药物治疗剂的患者，以检测针对药物的自身抗体（例如，HACA和/或HAHA）的存在或水平。

    Detecting and profiling molecular complexes
    5.
    发明授权
    Detecting and profiling molecular complexes 有权
    检测和分析分子复合物

    公开(公告)号：US08198031B2

    公开(公告)日：2012-06-12

    申请号：US11717760

    申请日：2007-03-12

    申请人： Po-Ying Chan-Yui ,  Sharat Singh ,  Hossein Salimi-Moosavi ,  Yining Shi ,  Sailaja Pidaparthi ,  Rajiv Dua ,  Ali Mukherjee

    发明人： Po-Ying Chan-Yui ,  Sharat Singh ,  Hossein Salimi-Moosavi ,  Yining Shi ,  Sailaja Pidaparthi ,  Rajiv Dua ,  Ali Mukherjee

    IPC分类号： G01N33/53 ,  G01N33/532 ,  G01N33/554 ,  C07K1/10 ,  C07K1/14

    CPC分类号： G01N33/566 ,  G01N33/542 ,  Y10S436/824

    摘要： Methods are provided for detecting the formation of complexes of molecules, especially proteins, in a sample, such as a cell or tissue lysate. In one aspect, a cleaving probe specific for a first protein in a complex and one or more binding compounds specific for one or more second proteins in a complex are provided. Upon binding, the cleaving probe is induced to generate an active species, such as singlet oxygen, that cleaves molecular tags attached to the binding compounds only in the local region of the cleaving probe. The released molecular tags are separated from the assay mixture and from one another to provide a readout that is related to the number and types of proteins present in the complex.

    摘要翻译： 提供了用于检测样品（例如细胞或组织裂解物）中分子复合物（特别是蛋白质）的形成的方法。 在一个方面，提供了对复合物中的第一种蛋白质特异性的切割探针和一种或多种特异于复合物中的一种或多种第二种蛋白质的结合化合物。 在结合时，诱导切割探针以产生仅在切割探针的局部区域中切割附着于结合化合物的分子标签的活性物质，例如单线态氧。 将释放的分子标签与测定混合物和彼此分离，以提供与复合物中存在的蛋白质的数量和类型相关的读数。

    METHODS FOR DIAGNOSING IRRITABLE BOWEL SYNDROME
    6.
    发明申请
    METHODS FOR DIAGNOSING IRRITABLE BOWEL SYNDROME 有权
    用于诊断可刺激性牙刷综合征的方法

    公开(公告)号：US20110159521A1

    公开(公告)日：2011-06-30

    申请号：US12862707

    申请日：2010-08-24

    申请人： Hua Gong ,  Shui Long Wang ,  Sharat Singh

    发明人： Hua Gong ,  Shui Long Wang ,  Sharat Singh

    IPC分类号： G01N33/573 ,  G01N33/53 ,  G01N33/92

    CPC分类号： G01N33/573 ,  G01N33/6893 ,  G01N33/74 ,  G01N2800/065 ,  G01N2800/56 ,  Y10S436/809 ,  Y10T436/147777

    摘要： The invention provides an ELISA assay for the determination of serum mast cell β-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of β-tryptase, histamine and/or prostaglandin E2.

    摘要翻译： 本发明提供了使用兔抗类胰蛋白酶作为捕获抗体和碱性磷酸酶缀合的G3作为检测抗体的ELISA测定法来测定血清肥大细胞和bgr-酪氨酸酶水平。 使用发光底物CPSD增强测定灵敏度。 还提供了通过检测血清中β-谷氨酸酶，组胺和/或前列腺素E2的水平来帮助诊断肠易激综合征的方法。

    Response Predictors for Erbb Pathway-Specific Drugs
    9.
    发明申请
    Response Predictors for Erbb Pathway-Specific Drugs 审中-公开
    Erbb途径特异性药物的反应预测因子

    公开(公告)号：US20080254497A1

    公开(公告)日：2008-10-16

    申请号：US11577180

    申请日：2005-10-17

    申请人： Sharat Singh

    发明人： Sharat Singh

    IPC分类号： C12Q1/02

    CPC分类号： G01N33/57492 ,  G01N2800/52

    摘要： The invention provides a method of determining whether tumor cells or tissue is responsive to treatment with an ErbB pathway-specific drug. In accordance with the invention, measurements are made on such cells or tissues to determine values for total ErbB receptors of one or more types, ErbB receptor dimers of one or more types and their phosphorylation states, and/or one or more ErbB signaling pathway effector proteins and their phosphorylation states. These quantities, or a response index based on them, are positively or negatively correlated with cell or tissue responsiveness to treatment with an ErbB pathway-specific drug. In one aspect, such correlations are determined from a model of the mechanism of action of a ErbB pathway-specific drug on an ErbB pathway. Preferably, methods of the invention are implemented by using sets of binding compounds having releasable molecular tags that are specific for multiple components of one or more complexes formed in ErbB pathway activation. After binding, molecular tags are released and separated from the assay mixture for analysis.

    摘要翻译： 本发明提供了确定肿瘤细胞或组织是否对用ErbB途径特异性药物治疗有反应的方法。 根据本发明，对这样的细胞或组织进行测量以确定一种或多种类型的ErbB受体的一个或多个类型的ErbB受体二聚体及其磷酸化状态和/或一个或多个ErbB信号传导途径效应物的值 蛋白质及其磷酸化状态。 这些量或基于它们的反应指数与用ErbB途径特异性药物治疗的细胞或组织反应性呈正相关或负相关。 在一个方面，这种相关性是根据ErbB途径特异性药物在ErbB途径上的作用机理的模型来确定的。 优选地，本发明的方法通过使用具有可释放分子标签的结合化合物组来实现，所述可释放分子标签对ErbB通路激活中形成的一种或多种复合物的多种成分是特异性的。 结合后，分离标签被释放并与测定混合物分离，用于分析。

    Methods and compositions for detecting the activation states of multiple signal transducers in rare circulating cells
    10.
    发明申请
    Methods and compositions for detecting the activation states of multiple signal transducers in rare circulating cells 审中-公开
    用于检测稀有循环细胞中多个信号传感器的激活状态的方法和组合物

    公开(公告)号：US20080076139A1

    公开(公告)日：2008-03-27

    申请号：US11525598

    申请日：2006-09-21

    申请人： Sharat Singh

    发明人： Sharat Singh

    IPC分类号： G01N33/574

    CPC分类号： G01N33/57484 ,  G01N33/54306 ,  G01N33/57496

    摘要： Methods and kits for detecting the activation states of a plurality of signal transducers of circulating cells of a solid tumor in a specific, multiplex, high-throughput assay are described. The methods comprise: contacting the signal transducers extracted from the cells with first, second, and third binding partners specific for each of the signal transducers to produce signal transducer-binding partner complexes. The second binding partners bind the corresponding signal transducers independent of their activation state and are labeled with a first moiety, and the third binding partners bind the corresponding signal transducers dependent of their activation state and are labeled with a second moiety. The first and second moieties are detected as an indication of the activation states of the plurality of signal transducers.

    摘要翻译： 描述了用于在特定的，多重的，高通量测定中检测实体瘤的循环细胞的多个信号转导体的激活状态的方法和试剂盒。 所述方法包括：将从细胞提取的信号转导体与第一，第二和第三结合伴侣接触，以对每个信号转导子特异性产生信号转导子结合配偶体复合物。 第二结合配偶体独立于它们的活化状态结合对应的信号转导体，并用第一部分标记，并且第三结合配偶体依赖于其激活状态结合对应的信号转导体并用第二部分标记。 检测第一和第二部分作为多个信号换能器的激活状态的指示。