摘要:
There are provided a DNA construct comprising non-eukaryote-derived suppressor tRNA gene containing no internal promoter functioning in a eukaryotic cell, and a eukaryote-derived or bacteriophage-derived promoter linked at the 5′ end of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing a non-natural amino acid-incorporated protein by using the same.
摘要:
There are provided a DNA construct comprising a suppressor tRNA gene of a non-eukaryote containing no internal promoter functioning in a eukaryotic cell, and a eukaryotic or bacteriophage promoter linked at the 5′ end of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing protein incorporating a non-natural amino acid by using the same.
摘要:
A first MOS transistor includes, as a first impurity region, a pair of first source/drain regions including first portions formed in a semiconductor substrate and second portions formed so as to project upward from the first portions. A second MOS transistor includes a pair of second source/drain regions including second impurity regions formed in the semiconductor substrate, third impurity regions located in contact with the second impurity regions so as to project upward from the semiconductor substrate, and fourth impurity regions located on the third impurity regions. The concentration of impurities in the third impurity regions is lower than that of impurities in the fourth impurity regions. The concentration of impurities in the first impurity regions is lower than that of impurities in the second impurity regions. The first, the second, the third and the fourth impurity regions are same conductivity type.
摘要:
In order to provide a membrane protein production method which does not require the step of solubilizing a membrane protein and which allows the membrane protein having an excellent quality to be obtained with a high yield, a method in accordance with an embodiment of the present invention includes: a step (a) of preparing a reaction solution for cell-free protein synthesis, the reaction solution containing (i) a template nucleic acid which encodes the membrane protein, (ii) a lipid, and (iii) a detergent which is contained at a concentration equal to or higher than a critical micelle concentration; and a step (b) of synthesizing the membrane protein while the concentration of the detergent in the reaction solution is maintained at a concentration equal to or higher than a critical micelle concentration.
摘要:
A simple and efficient method of preparing a sample in the measurement according to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) capable of inhibiting any ion suppression by impurities, such as inorganic salts and surfactants, contained in the sample. An analyte and matrix molecules are co-crystallized in the presence of porous microparticles. Preferably, this co-crystallization is carried out by bringing the analyte, matrix molecules and porous microparticles into contact with each other on a target plate and thereafter drying the mixture. The porous microparticles consist of an ion exchanger having an average particle diameter of not more than 50 μm, preferably a strongly basic anion exchanger.
摘要:
A semiconductor device includes a double gate transistor which comprises an active region of a fin type and a pair of gate electrodes disposed opposite to each other through the active region. A height of the gate electrodes is higher than that of the active region and equal to or smaller than a calculated gate electrode height calculated using the following formula: ( ( Gate Electrode Height [ nm ] ) - ( Active Region Height [ nm ] ) ) / ( Active Region Height [ nm ] ) = 3.5 - 5 × ( Gate Length [ nm ] ) 2 - 0.002 × ( Gate Length [ nm ] ) + 0.16 .