公开(公告)号：US20050158848A1

公开(公告)日：2005-07-21

申请号：US10511192

申请日：2003-04-10

申请人： Ulrich Sieben ,  Ingo Freund ,  Holger Klapproth

发明人： Ulrich Sieben ,  Ingo Freund ,  Holger Klapproth

IPC分类号： B01J20/32 ,  C09D125/06 ,  C09D179/08 ,  C09D201/00 ,  G01N33/543 ,  G01N37/00 ,  C12M1/34 ,  B05D3/00

CPC分类号： B01J20/3204 ,  B01J20/103 ,  B01J20/28011 ,  B01J20/3257 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3276 ,  B01J20/3289 ,  G01N33/54393

摘要： In a method for immobilizing molecules on a surface, a layer of a hydrophobic, e.g., a non-swelling polymer, may be applied to the surface and molecules are immobilized on a surface of the polymer layer. The hydrophobic polymers may be polyimide or polystyrene, and the surface to which the polymer layer is applied may be made of an inorganic material, e.g., a semiconductor material (silicon), a semiconducting oxide (silicon dioxide), glass, nitride, or ceramic. Hydrophobic polymers such as polyimide or polystyrene can be applied to the surface of an inorganic support with conventional methods. Further, they electrically insulate the support with respect to the molecules applied to the surface of the polymer layer or substances associated with these molecules. Thus, electrical sensors and processor circuits can be integrated into the support without any detrimental effect on their function due to molecules and substances applied to the surface of the polymer layer.

摘要翻译： 在将分子固定在表面上的方法中，可以将一层疏水的，例如不溶胀的聚合物施加到表面上，并将分子固定在聚合物层的表面上。 疏水聚合物可以是聚酰亚胺或聚苯乙烯，并且施加聚合物层的表面可以由无机材料制成，例如半导体材料（硅），半导体氧化物（二氧化硅），玻璃，氮化物或陶瓷 。 疏水性聚合物如聚酰亚胺或聚苯乙烯可以用常规方法施加到无机载体的表面上。 此外，它们相对于施加到聚合物层的表面上的分子或与这些分子相关联的物质将载体电绝缘。 因此，电传感器和处理器电路可以集成到支撑件中，而不会由于施加到聚合物层表面的分子和物质而对它们的功能产生不利影响。

公开(公告)号：US20050176064A1

公开(公告)日：2005-08-11

申请号：US10520039

申请日：2003-06-30

申请人： Holger Klapproth ,  Ulrich Sieben

发明人： Holger Klapproth ,  Ulrich Sieben

IPC分类号： B01J19/00 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  G01N33/542 ,  G01N33/543 ,  G01N33/551 ,  G01N33/566 ,  G01N33/58 ,  G01N33/53

CPC分类号： G01N33/54306 ,  B01J19/0046 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00578 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/00677 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B01J2219/00734 ,  B01J2219/0074 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  G01N33/542 ,  G01N33/543 ,  G01N33/551 ,  G01N33/566 ,  G01N33/582

摘要： The invention relates to a method for determining the number of receptors on a carrier, in addition to a biosensor, especially a protein sensor, which can be produced using the method.

摘要翻译： 本发明涉及一种用于确定载体上的受体数量的方法，除了可以使用该方法生产的生物传感器，特别是蛋白质传感器。

公开(公告)号：US20090017505A1

公开(公告)日：2009-01-15

申请号：US11805242

申请日：2007-05-22

申请人： Ulrich Sieben ,  Holger Klapproth

发明人： Ulrich Sieben ,  Holger Klapproth

IPC分类号： C12P19/34 ,  C12M1/40 ,  C12M1/34

CPC分类号： B01L3/0262 ,  B01F13/0071 ,  B01F13/0084 ,  B01F15/06 ,  B01L3/5085 ,  B01L3/5088 ,  B01L3/563 ,  B01L2200/025 ,  B01L2200/143 ,  B01L2300/0819 ,  B01L2300/1822

摘要： In a process for mixing microdroplets (2a, 2b, 2c), at least two carriers (3a, 3b) are provided whose surfaces (4a, 4b) are each structured in such a way that at least one hydrophilic surface domain (5a, 5b) is delimited by at least one hydrophobic surface domain (6a, 6b). A first microdroplet (2a, 2b, 2c) is disposed on a hydrophilic surface domain (5a) of a first carrier (3a), and a second microdroplet (2a, 2b, 3c) is disposed on a hydrophilic surface domain (5b) of a second carrier (3b). The carriers (3a, 3b), with their first and second hydrophilic surfaces (4a, 4b) facing each other, are positioned adjacent to each other and sufficiently close to each other by being moved toward each other that the microdroplets (2a, 2b, 2c) come into contact with each other.

摘要翻译： 在混合微滴（2a，2b，2c）的方法中，提供至少两个载体（3a，3b），其表面（4a，4b）各自构造成使得至少一个亲水性表面区域（5a，5b） ）由至少一个疏水性表面结构域（6a，6b）界定。 第一微滴（2a，2b，2c）设置在第一载体（3a）的亲水性表面区域（5a）上，第二微滴（2a，2b，3c）设置在亲水性表面区域 第二载体（3b）。 其第一和第二亲水表面（4a，4b）彼此面对的载体（3a，3b）通过彼此移动彼此相邻并且彼此靠近彼此，使得微滴（2a，2b， 2c）相互接触。

公开(公告)号：US20080286882A1

公开(公告)日：2008-11-20

申请号：US11960090

申请日：2007-12-19

申请人： Ulrich Sieben ,  Holger Klapproth

发明人： Ulrich Sieben ,  Holger Klapproth

IPC分类号： G01N33/543 ,  B01J19/00

CPC分类号： G01N33/54366

摘要： In a method for measuring the concentration of a ligand contained in a sample that is to be tested, at a plurality of test sites that are located on the surface of at least one substrate, in each case at least one receptor that is suitable upon contact with the ligand for specifically binding to said ligand, is immobilized. The sample is brought into contact for a predetermined time with the test sites in such a way that in the sample differently sized diffusion volumes are adjacent to the receptors from which at least one ligand can diffuse to the respective receptor during the predetermined time. After the predetermined time has passed, for each test site a measured signal for the number or density of the binding events at the test site is acquired.

摘要翻译： 在用于测量待测试样品中所含配体浓度的方法中，在位于至少一个底物表面上的多个测试位点，在每种情况下至少一种适合接触的受体 与特异性结合所述配体的配体被固定化。 使样品与测试位置接触预定时间，使得在样品中不同尺寸的扩散体积与受体相邻，在预定时间内至少一种配体可以从该受体扩散到相应的受体。 在预定时间过去之后，对于每个测试站点，获取测试点处的绑定事件的数量或密度的测量信号。

公开(公告)号：US20070107514A1

公开(公告)日：2007-05-17

申请号：US11545778

申请日：2006-10-10

申请人： Ulrich Sieben ,  Holger Klapproth ,  Mirko Lehmann

发明人： Ulrich Sieben ,  Holger Klapproth ,  Mirko Lehmann

IPC分类号： G01F15/14

CPC分类号： G01N21/6454

摘要： An integrated sensor array has a substrates, at least one sensor element, and a signal processing device integrated in the substrate and connected or capable of being connected with the sensor elements. A layer having a measuring surface is capable of being detachably bonded to the substrate so that a signal emitted from the measuring surface is capable of being processed by the signal processing device when the layer is bonded to the substrate. The substrate has at least one feedthrough hole abutting with the layer, which feedthrough hole is connected or capable of being connected to a vacuum generating device in order to bond the layer to the substrate by suction.

摘要翻译： 集成传感器阵列具有基板，至少一个传感器元件和集成在基板中并连接或能够与传感器元件连接的信号处理装置。 具有测量表面的层能够可拆卸地结合到基板，使得当该层与基板结合时，从测量表面发射的信号能够被信号处理装置处理。 衬底具有与该层邻接的至少一个馈通孔，所述馈通孔连接或能够连接到真空产生装置，以便通过抽吸将层粘合到衬底。

公开(公告)号：US08329104B2

公开(公告)日：2012-12-11

申请号：US12146644

申请日：2008-06-26

申请人： Mirko Lehmann ,  Ingo Freund ,  Sonja Mohry ,  Holger Klapproth

发明人： Mirko Lehmann ,  Ingo Freund ,  Sonja Mohry ,  Holger Klapproth

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/566 ,  G01N33/543

CPC分类号： G01N33/54373 ,  G01D3/08 ,  G01N27/414 ,  G01N27/4163 ,  G01N33/0032

摘要： A measurement device has at least one sensor for detecting a parameter, a data storage unit, and a power supply for said sensor and data storage unit. In addition to the first sensor, the measurement device has at least one second sensor connected to the power supply and to the data storage unit. The second sensor is provided for detecting a physical quantity acting on the first sensor and is capable of permanently altering the measurement characteristic of the first sensor. An evaluator functions coactively with the second sensor and the data storage unit so that the permanent alteration of the measurement characteristic of the first sensor is recordable as a function of the measurement signal of the second sensor.

摘要翻译： 测量装置具有用于检测参数的至少一个传感器，用于所述传感器和数据存储单元的数据存储单元和电源。 除了第一传感器之外，测量装置具有连接到电源和数据存储单元的至少一个第二传感器。 提供第二传感器用于检测作用在第一传感器上的物理量，并且能够永久地改变第一传感器的测量特性。 评估器与第二传感器和数据存储单元共同作用，使得可以根据第二传感器的测量信号来记录第一传感器的测量特性的永久变化。

公开(公告)号：US20090175764A1

公开(公告)日：2009-07-09

申请号：US12146644

申请日：2008-06-26

申请人： Mirko Lehmann ,  Ingo Freund ,  Sonja Mohry ,  Holger Klapproth

发明人： Mirko Lehmann ,  Ingo Freund ,  Sonja Mohry ,  Holger Klapproth

IPC分类号： B01J19/00

CPC分类号： G01N33/54373 ,  G01D3/08 ,  G01N27/414 ,  G01N27/4163 ,  G01N33/0032

摘要： A measurement device has at least one sensor for detecting a parameter, a data storage unit, and a power supply for said sensor and data storage unit. In addition to the first sensor, the measurement device has at least one second sensor connected to the power supply and to the data storage unit. The second sensor is provided for detecting a physical quantity acting on the first sensor and is capable of permanently altering the measurement characteristic of the first sensor. An evaluator functions coactively with the second sensor and the data storage unit so that the permanent alteration of the measurement characteristic of the first sensor is recordable as a function of the measurement signal of the second sensor.

摘要翻译： 测量装置具有用于检测参数的至少一个传感器，用于所述传感器和数据存储单元的数据存储单元和电源。 除了第一传感器之外，测量装置具有连接到电源和数据存储单元的至少一个第二传感器。 提供第二传感器用于检测作用在第一传感器上的物理量，并且能够永久地改变第一传感器的测量特性。 评估器与第二传感器和数据存储单元共同作用，使得可以根据第二传感器的测量信号来记录第一传感器的测量特性的永久变化。

公开(公告)号：US07312867B2

公开(公告)日：2007-12-25

申请号：US10529048

申请日：2003-09-12

申请人： Holger Klapproth ,  Mirko Lehmann

发明人： Holger Klapproth ,  Mirko Lehmann

IPC分类号： G01J3/30

CPC分类号： G01N21/6454 ,  G01N21/6428 ,  G01N21/648 ,  H01L2924/0002 ,  H01L2924/00

摘要： Disclosed is a device for detecting at least one luminescent substance, comprising a radiation source for emitting excitation radiation to the at least one luminescent substance. The excitation radiation is provided with at least one excitation wavelength at which the luminescent substance is excited so as to emit luminescent radiation. At least one radiation receiver is provided, which detects the luminescent radiation and is configured regarding the spectral sensitivity thereof in such a way that said radiation receiver is insensitive to the radiation emitted by the radiation source. The luminescent substance is located inside a measuring chamber that is essentially impermeable to the luminescent radiation and comprises at least one wall area which is transparent to the excitation radiation emitted by the radiation source. The radiation source is placed outside the measuring chamber such that the excitation radiation emitted by the radiation source is coupled into the inside of the measuring chamber by penetrating said wall area.

摘要翻译： 公开了一种用于检测至少一种发光物质的装置，包括用于向至少一种发光物质发射激发辐射的辐射源。 激发辐射具有至少一个激发波长，在该激发波长处激发发光物质以发射发光辐射。 提供至少一个辐射接收器，其检测发光辐射，并且被配置为关于其光谱灵敏度，使得所述辐射接收器对由辐射源发射的辐射不敏感。 发光物质位于测量室内，其基本上不透气于发光辐射，并且包括对由辐射源发射的激发辐射透明的至少一个壁区域。 辐射源被放置在测量室的外部，使得由辐射源发射的激发辐射通过穿透所述壁区域而耦合到测量室的内部。

公开(公告)号：US20060141445A1

公开(公告)日：2006-06-29

申请号：US11316674

申请日：2005-12-22

申请人： Holger Klapproth

发明人： Holger Klapproth

IPC分类号： C12Q1/00

CPC分类号： G01N33/54353 ,  G01N33/54393

摘要： In a process for manufacturing a sensor chip a support is prepared having at least one surface region in which at least one reactive group is arranged. A buffer solution is applied to the surface region for coating at least one surface region. Then, at least one receptor having binding specificity for a ligand to be detected is brought into contact with the surface coating so obtained in such a manner that said receptor binds covalently to at least one reactive group.

摘要翻译： 在制造传感器芯片的方法中，制备具有至少一个表面区域的载体，其中布置有至少一个反应性基团。 将缓冲溶液施加到表面区域以涂覆至少一个表面区域。 然后，使具有对待检测配体具有结合特异性的至少一种受体与如此获得的表面涂层接触，使得所述受体共价结合至至少一个反应性基团。

公开(公告)号：US20060115906A1

公开(公告)日：2006-06-01

申请号：US10521817

申请日：2003-07-21

申请人： Holger Klapproth

发明人： Holger Klapproth

IPC分类号： G01N33/543

CPC分类号： G01N33/54353 ,  G01N33/54373 ,  G01N33/54393

摘要： The present invention concerns a sensor surface with an improved signal/noise ratio that is based on a blocking reagent covalently immobilized on the sensor surface, as well as apparatuses comprising such surfaces and methods for the use of same.

摘要翻译： 本发明涉及一种基于共价固定在传感器表面上的封闭剂，具有改进的信噪比的传感器表面以及包括这些表面的设备及其使用方法。