公开(公告)号：US20100099900A1

公开(公告)日：2010-04-22

申请号：US12530839

申请日：2008-04-10

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: C12N15/82 ,  C07H21/04 ,  C12N5/04 ,  A01H5/00 ,  C07F9/08 ,  C12P7/64 ,  A01H5/10

CPC classification number: C12P7/6427 ,  C12N9/0083 ,  C12N15/8247

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) and using these delta-8 desaturases in plants.

Abstract translation: 分离的核酸片段和包含编码δ-8去饱和酶的片段以及制备长链多不饱和脂肪酸（PUFA）的方法并在植物中使用这些δ-8去饱和酶的重组构建体。

公开(公告)号：US07695950B2

公开(公告)日：2010-04-13

申请号：US11748637

申请日：2007-05-15

Applicant: Howard Glenn Damude ,  Dana M. Walters Pollak ,  Zhixiong Xue ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Dana M. Walters Pollak ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC: C12N1/00 ,  C12N15/74 ,  C07H21/04

CPC classification number: C12N9/0083

Abstract: The present invention relates to a Δ5 desaturase, which has the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ5 desaturase in oleaginous yeast are disclosed.

Abstract translation: 本发明涉及一种具有将二硫-γ-亚麻酸（DGLA; 20：3ω-6）转化成花生四烯酸（ARA; 20：4ω-6）和/或二十碳四烯酸 酸（ETA; 20：4ω-3）至二十碳五烯酸（EPA; 20：5ω-3）。 公开了分离的核酸片段和重组构建体，其包含编码“Dgr。5去饱和酶”的片段以及在含油酵母中使用该Dgr。5去饱和酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20100022647A1

公开(公告)日：2010-01-28

申请号：US12512507

申请日：2009-07-30

Applicant: Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  Dana M. Walters Pollak ,  James John Ragghianti ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  Dana M. Walters Pollak ,  James John Ragghianti ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

IPC: A61K31/20 ,  C12N1/19 ,  C07C57/04 ,  A23D9/00 ,  A23C23/00 ,  A23K1/00

CPC classification number: C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.

Abstract translation: 描述了能够在总油馏分中产生大于10％花生四烯酸（ARA，ω-6多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包含表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使ARA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内产生ARA的方法。

公开(公告)号：US07645604B2

公开(公告)日：2010-01-12

申请号：US11601564

申请日：2006-11-16

Applicant: Howard Glenn Damude ,  Brian McGonigle ,  Zhixiong Xue ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Brian McGonigle ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC: C12N9/10 ,  C12P7/64 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C12N15/8247 ,  C11B1/025 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in plants and oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将亚油酸[18:2，LA]转化成二十碳烯酸[20：2，EDA]的能力的Δ9延伸酶。 公开了分离的核酸片段和包含编码Δ9延伸酶的这种片段的重组构建物以及在植物和油质酵母中使用这些Delta9延伸酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20090176003A1

公开(公告)日：2009-07-09

申请号：US11951697

申请日：2007-12-06

Applicant: Howard Glenn Damude ,  Hongxian He ,  Der-ing Liao ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Hongxian He ,  Der-ing Liao ,  Quinn Qun Zhu

IPC: C12N15/82 ,  C12N15/11 ,  C12N15/00 ,  C12N5/04 ,  A01H5/00 ,  C12P7/64 ,  A23D9/007 ,  A23L1/30 ,  A23K1/16

CPC classification number: C12N15/8247 ,  C12N9/0083

Abstract: The present invention relates to mutant delta-8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 omega-6, EDA] to dihomo-γ-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 omega-3, ETrA] to eicosatetraenoic acid [20:3 omega-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant delta-8 desaturases in plants and oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将二十碳二烯酸[20:2ω-6，EDA]转化为二高-γ-亚麻酸[20:3，DGLA]和/或二十碳三烯酸[...]的能力的突变体δ-8去饱和酶基因[ 20：3ω-3，ETrA]至二十碳四烯酸[20：3ω-3，ETA]。 公开了分离的核酸片段和重组构建体，其包含编码δ-8去饱和酶的片段以及使用植物中的这些突变型δ-8去饱和酶和油性酵母制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20080295204A1

公开(公告)日：2008-11-27

申请号：US12126161

申请日：2008-05-23

Applicant: Knut Meyer ,  William D. Hitz ,  Narendra S. Yadav ,  Howard Glenn Damude

Inventor： Knut Meyer ,  William D. Hitz ,  Narendra S. Yadav ,  Howard Glenn Damude

IPC: C12N15/54 ,  A01H5/00 ,  C12N5/10

CPC classification number: C12N15/8247 ,  C12N9/1029 ,  C12Y203/01158

Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia Lipolytica are used to achieve the increase in seed storage lipids.

Abstract translation: 描述了与非转基因，无偏分离大豆种子的总脂肪酸含量相比，具有至少10％的总脂肪酸含量增加和改变的脂肪酸分布的转基因大豆种子。 来自解脂耶氏酵母的DGAT基因用于实现种子储存脂质的增加。

公开(公告)号：US20080254522A1

公开(公告)日：2008-10-16

申请号：US12102879

申请日：2008-04-15

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: C12P7/64 ,  C12N1/00 ,  C12N1/15 ,  C07H21/04 ,  C12N1/11

CPC classification number: C12N9/1029 ,  A23D9/00 ,  A23D9/007 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  A23V2250/1862 ,  A23V2250/187 ,  A23V2250/1868

Abstract: The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid (LA; 18:2 ω-6) to eicosadienoic acid (EDA; 20:2 ω-6) and/or α-linolenic acid (ALA; 18:3 ω-3) to eicosatrienoic acid (ETrA; 20:3 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将亚油酸（LA; 18：2ω-6）转化成二十碳烯酸（EDA; 20：2ω-6）和/或α-亚麻酸（ALA; 18）的能力的Δ9延伸酶 ：3ω-3）至二十碳三烯酸（ETrA; 20：3ω-3）。 公开了分离的核酸片段和包含编码Δ9延伸酶的这种片段的重组构建物以及在含油酵母中使用这些Delta9延伸酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20080095915A1

公开(公告)日：2008-04-24

申请号：US11876115

申请日：2007-10-22

Applicant: Howard Glenn Damude ,  Zhixiong Xue ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC: A23L1/00 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C07H21/00 ,  C12N15/63 ,  C12P7/64 ,  C12N1/00 ,  A23D7/00 ,  A23J7/00 ,  A23K1/00

CPC classification number: C12N15/8247 ,  A23K20/158 ,  A23L33/12 ,  C12N9/0083

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-8 desaturases in plants and oleaginous yeast.

Abstract translation: 分离的核酸片段和包含编码δ-8去饱和酶的片段的重组构建物以及使用这些δ-8去饱和酶在植物和油质酵母中制备长链多不饱和脂肪酸（PUFA）的方法。