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    Plant virus transmission inhibitor and methods
    91.
    发明申请
    Plant virus transmission inhibitor and methods 失效
    植物病毒传播抑制剂和方法

    公开(公告)号：US20090055975A1

    公开(公告)日：2009-02-26

    申请号：US12001776

    申请日：2007-12-11

    申请人： Bryony Bonning ,  W. Allen Miller ,  Sijun Liu

    发明人： Bryony Bonning ,  W. Allen Miller ,  Sijun Liu

    IPC分类号： C07K7/08 ,  C12N15/11 ,  C12N15/62 ,  A01H5/00 ,  A61P31/12

    CPC分类号： C07K7/08 ,  C07K14/005 ,  C12N15/8283 ,  C12N2770/00022

    摘要： Provided is a peptide, peptide multimer or fusion protein containing such peptide which binds to the gut of sap-sucking insects, e.g., aphids. When bound, this peptide inhibits the binding of targeted virus to the insect gut. When this peptide or tandem repeat peptide is expressed (or is expressed as part of a fusion protein) in the fluids of transgenic plants, it is taken up by the insect with the sap, binds to gut receptors and thereby inhibits transmission of the virus from plant to plant via the insect vector. Thus, the use of such transgenic plants blocks or reduces the transmission of the targeted virus and others which share the peptide binding site within the insect gut among plants susceptible to the virus and thereby reduces losses due to viral infection.

    摘要翻译： 提供了含有这种肽的肽，肽多聚体或融合蛋白，其结合吸吮昆虫的肠道，例如蚜虫。 当肽结合时，该肽抑制靶向病毒与昆虫肠道的结合。 当这种肽或串联重复肽在转基因植物的液体中表达（或作为融合蛋白的一部分表达）时，它被昆虫与树汁吸收，结合肠道受体，从而抑制病毒从 植物通过昆虫载体植物。 因此，这种转基因植物的使用阻止或减少在易感病毒的植物中在昆虫肠内共享肽结合位点的目标病毒和其他物质的传播，从而减少由于病毒感染引起的损失。

    NUCLEIC ACID MOLECULES ENCODING MISMATCH ENDONUCLEASES AND METHODS OF USE THEREOF
    93.
    发明申请
    NUCLEIC ACID MOLECULES ENCODING MISMATCH ENDONUCLEASES AND METHODS OF USE THEREOF 审中-公开
    编码缺失缺失突变体的核酸分子及其使用方法

    公开(公告)号：US20080145913A1

    公开(公告)日：2008-06-19

    申请号：US11860465

    申请日：2007-09-24

    申请人： Hal S. Padgett ,  Andrew A. Vaewhongs

    发明人： Hal S. Padgett ,  Andrew A. Vaewhongs

    IPC分类号： C12N9/18

    CPC分类号： C12N15/102 ,  C07K14/005 ,  C12N9/22 ,  C12N15/1027 ,  C12N15/8203 ,  C12N15/8257 ,  C12N2770/00022

    摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. Also described are mismatch endonucleases suitable for use in the process.

    摘要翻译： 我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。 该方法使用相反链的退火以形成具有错配的多核苷酸双链体。 将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。 并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。 任选地连接得到的多核苷酸。 产生几种变异多核苷酸。 在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。 还描述了适用于该方法的不匹配核酸内切酶。

    Method of conveying BNYVV resistance to sugar beet plants
    96.
    发明授权
    Method of conveying BNYVV resistance to sugar beet plants 有权
    传播BNYVV对甜菜植物抗性的方法

    公开(公告)号：US06956149B1

    公开(公告)日：2005-10-18

    申请号：US09889938

    申请日：2000-01-26

    申请人： Kenneth Richards ,  Gérard Jonard ,  Hubert Guilley ,  Cornelis Maria Petrus Van Dun

    发明人： Kenneth Richards ,  Gérard Jonard ,  Hubert Guilley ,  Cornelis Maria Petrus Van Dun

    IPC分类号： A01H5/00 ,  C07K14/08 ,  C12N5/10 ,  C12N15/40 ,  C12N15/82 ,  A01H5/10 ,  C12N15/90

    CPC分类号： C07K14/005 ,  C12N15/8283 ,  C12N2770/00022

    摘要： The present invention relates to a method for conveying resistance to beet necrotic yellow vein virus (BNYVV) to a sugar beet plant, which method comprises the following steps: (a) preparing a DNA fragment consisting of a nucleotide sequence that corresponds to nucleotides 153 to 3258 of the genomic RNA 1 of the beet necrotic yellow vein virus (BNYVV); (b) introducing said DNA fragment, operatively linked to a promoter that is active in sugar beet plants, into a sugar beet plant cell to obtain a transformed sugar beet cell; and (c) regenerating a transgenic sugar beet plant from the transformed sugar beet plant cell.

    摘要翻译： 本发明涉及一种向甜菜植物输送甜菜坏死性黄色静脉病毒（BNYVV）的抗性的方法，该方法包括以下步骤：（a）制备由与核苷酸153相对应的核苷酸序列组成的DNA片段， 3258的甜菜坏死性黄色静脉病毒（BNYVV）的基因组RNA 1; （b）将可操作地连接到在甜菜植物中有活性的启动子的所述DNA片段引入甜菜植物细胞中以获得转化的甜菜细胞; 和（c）从转化的甜菜植物细胞再生转基因甜菜植物。

    Parvovirus vaccine as viral coat protein fusions
    99.
    发明授权
    Parvovirus vaccine as viral coat protein fusions 失效
    细小病毒疫苗作为病毒外壳蛋白融合

    公开(公告)号：US06730306B1

    公开(公告)日：2004-05-04

    申请号：US09520967

    申请日：2000-03-08

    申请人： Gregory P. Pogue ,  John A. Lindbo ,  Michael J. McCulloch ,  Jonathan E. Lawrence ,  Cynthia S. Gross ,  Stephen J. Garger

    发明人： Gregory P. Pogue ,  John A. Lindbo ,  Michael J. McCulloch ,  Jonathan E. Lawrence ,  Cynthia S. Gross ,  Stephen J. Garger

    IPC分类号： A61K3923

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K14/445 ,  C07K2319/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2770/00022

    摘要： The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.

    摘要翻译： 本发明涉及与重组植物病毒结构蛋白融合的外来肽序列及其生产方法。 融合蛋白在生物学含有的烟草花叶病毒的高水平植物中经济合成。 本发明的融合蛋白可用作诱导产生具有所需结合特性的抗体的抗原，例如保护性抗体，或用作诱导针对细小病毒的保护性免疫的疫苗抗原。 将猫细小病毒表位融合到TMV外壳蛋白的N末端，在烟草植物中表达，提取，纯化，表征并施用于动物，导致保护性免疫。