公开(公告)号：US4891133A

公开(公告)日：1990-01-02

申请号：US96506

申请日：1987-09-15

申请人： Arthur E. Colvin, Jr.

发明人： Arthur E. Colvin, Jr.

IPC分类号： B01D15/08 ,  G01N30/52 ,  G01N30/60

CPC分类号： B01D15/08 ,  G01N2030/522 ,  G01N30/6017 ,  G01N30/6021

摘要： Liquid chromatography apparatus including a hollow cylinder and a fixed end closure in which the effective useful length of the cylinder is varied through the use of a piston member that slides within the cylinder due to force exerted by a threaded shaft that is connected to a handle. Binding of the piston member in the cylinder is prevented by a ball joint that operatively connects the piston member to the threaded shaft and by a plurality of O-rings located on the piston member that contact the inner wall of the cylinder and tend to prevent tipping of the piston member. Even liquid distribution and filtering is obtained through a 0.5 micron porous sintered stainless steel liquid distribution disk. A smooth inclined surface in combination with an uninterrupted ring provide a trap for bubbles of gas located inside the cylinder. A composite porous segmented sintered liquid distribution disk is also presented that is particularly useful for large scale liquid chromatography as is the method of manufacturing the composite segmented disk.

摘要翻译： 液相色谱装置包括中空圆柱体和固定端盖，其中通过使用由连接到手柄的螺纹轴施加的力而在气缸内滑动的活塞构件改变气缸的有效有用长度。 活塞构件在气缸中的接合可通过球形接头来防止，该球窝接头可将活塞构件与螺纹轴和位于活塞构件上的多个O形环连接，该O形环接触气缸的内壁并倾向于防止倾翻 的活塞构件。 甚至通过0.5微米多孔烧结不锈钢液体分配盘获得液体分配和过滤。 与不间断环组合的平滑倾斜表面为位于气缸内部的气体气泡提供陷阱。 还提出了复合多孔分段烧结液体分配盘，其对于大规模液相色谱法是特别有用的，如制造复合分段盘的方法。

公开(公告)号：US4670141A

公开(公告)日：1987-06-02

申请号：US742265

申请日：1985-06-07

申请人： Carl L. Shackelford ,  Kenneth Rainin

发明人： Carl L. Shackelford ,  Kenneth Rainin

IPC分类号： G01N30/56 ,  B01D15/08 ,  G01N30/52 ,  G01N30/60

CPC分类号： G01N30/6021 ,  G01N30/52 ,  G01N30/6026 ,  G01N30/6039 ,  G01N2030/085 ,  G01N2030/522 ,  G01N30/6017 ,  G01N30/6034

摘要： A liquid chromatography column apparatus which utilizes sorbent packing material in a tube having a first end portion and a second end portion. The first end portion of the tube includes a plug having a passage therethrough. The plug fits in the tube adjacent the packing material and is movable within the tube. An end fitting is also employed adjacent the plug. The end fitting has a passage therethrough which communicates with the passage of the plug. A seal is used inside the tube between the tube and the end fitting. The second end portion of the tube is enclosed and also includes a passage from the interior to the exterior of the tube. A manually actuable gear mechanism is incorporated in the end fitting of the first end portion of the tube for facilitating application of axial force to the packing material, thereby compressing the packing material so as to eliminate voids and channels in the packing material. Other features are also disclosed.

摘要翻译： 一种在具有第一端部和第二端部的管中使用吸附剂包装材料的液相色谱柱装置。 管的第一端部包括具有穿过其的通道的塞子。 插头适合于与包装材料相邻的管中，并且可在管内移动。 在塞子附近也采用端部配件。 端部配件具有穿过其的通道，其与插头的通道连通。 在管和端部配件之间的管内使用密封。 管的第二端部被封闭，并且还包括从管的内部到外部的通道。 手动致动的齿轮机构结合在管的第一端部的端部配件中，以便于对包装材料施加轴向力，从而压缩包装材料，以消除包装材料中的空隙和通道。 还公开了其他特征。

公开(公告)号：US11927573B2

公开(公告)日：2024-03-12

申请号：US17156939

申请日：2021-01-25

申请人： CYTIVA BIOPROCESS R&D AB

发明人： Klaus Gebauer

IPC分类号： B01D15/18 ,  B01D15/20 ,  B01D15/22 ,  G01N30/56 ,  G01N30/60 ,  G01N30/86

CPC分类号： G01N30/6004 ,  B01D15/18 ,  B01D15/206 ,  B01D15/22 ,  G01N30/56 ,  G01N30/606 ,  G01N30/8658 ,  G01N2030/565 ,  G01N30/6017 ,  G01N30/6021 ,  G01N30/6052

摘要： The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US11841350B2

公开(公告)日：2023-12-12

申请号：US17695672

申请日：2022-03-15

申请人： Bio-Rad Laboratories, Inc.

发明人： Christopher Belisle ,  Mark A. Snyder

IPC分类号： B01D15/22 ,  G01N30/52 ,  G01N30/60

CPC分类号： G01N30/52 ,  B01D15/22 ,  G01N30/6021 ,  G01N2030/522

摘要： A dynamic axial compression column is disclosed herein. This dynamic axial column utilized external compression to prevent the creation of end plate space in the column. The dynamic axial column can include a tube defining a first opening, a second opening, and a lumen extending there between. The dynamic axial column can include a first end plate assembly sealing the first opening and movably extending at least partially into the lumen via the first opening, a second end plate assembly sealing the second opening, a plurality of rods extending along the outside of the tube and connecting the first end plate assembly and the second end plate assembly, and a first plurality of compression devices external to the tube and engaging one of the plurality of rods to bias the first end plate assembly towards the second end plate assembly.

公开(公告)号：US20180356375A1

公开(公告)日：2018-12-13

申请号：US16103149

申请日：2018-08-14

申请人： GE Healthcare BioProcess R&D AB

发明人： Klaus Gebauer

IPC分类号： G01N30/60 ,  B01D15/22 ,  B01D15/18

CPC分类号： G01N30/6004 ,  B01D15/18 ,  B01D15/22 ,  G01N30/6017 ,  G01N30/6021 ,  G01N30/6052 ,  G01N30/606

摘要： The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US20170030872A1

公开(公告)日：2017-02-02

申请号：US15303095

申请日：2015-03-30

申请人： GE Healthcare Bio-Sciences AB

发明人： Manoj Ramakrishna ,  Kishore Chengalvarayan ,  Nikhil Kamble

IPC分类号： G01N30/60 ,  B01D15/22

CPC分类号： G01N30/6047 ,  B01D15/22 ,  G01N30/6021 ,  G01N2030/027

摘要： A holding system for holding and hoisting a chromatography column of one or more chromatography columns is disclosed. The holding system includes an upright member and a plurality of holding members movably arranged along the upright member. A first supporting member is positioned at an end portion of the chromatography column, wherein a holding member of the plurality of holding members is configured to be engaged to the first supporting member. A second supporting member is positioned at another end portion of the chromatography column. A holding member of the plurality of holding members is configured to be engaged to the second supporting member. One or more hoist assisting members may be also present. A hoist assisting member is operatively connected to the holding member engaged to the first supporting member. The hoist assisting member is operable to move the holding member connected to the first supporting member for hoisting the chromatography column.

摘要翻译： 公开了用于保持和提升一个或多个色谱柱色谱柱的保持系统。 保持系统包括直立构件和沿着直立构件可移动地布置的多个保持构件。 第一支撑构件位于色谱柱的端部，其中多个保持构件的保持构件被配置为接合到第一支撑构件。 第二支撑构件位于色谱柱的另一端部。 多个保持构件的保持构件被构造成与第二支撑构件接合。 一个或多个起重机辅助部件也可以存在。 升降机辅助构件可操作地连接到与第一支撑构件接合的保持构件。 起重辅助构件可操作地移动连接到第一支撑构件的保持构件以提升色谱柱。

公开(公告)号：US09527009B2

公开(公告)日：2016-12-27

申请号：US14252874

申请日：2014-04-15

申请人： GE HEALTHCARE BIO-SCIENCES AB

发明人： Klaus Gebauer

IPC分类号： G01N30/60 ,  G01N30/38 ,  B01D15/22 ,  B01D15/18 ,  B01D15/26

CPC分类号： G01N30/6004 ,  B01D15/18 ,  B01D15/22 ,  G01N30/6017 ,  G01N30/6021 ,  G01N30/6052 ,  G01N30/606

摘要： The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

摘要翻译： 本发明涉及轴流色谱柱，通过使用这种色谱柱分离液体中的一种或多种分析物的方法，以及采用这种色谱柱的系统。 该柱包括第一端口和第二端口，第一端口和第二端口在色谱柱上的床空间的水平以上基本相同的水平或高度。

公开(公告)号：US20160214032A1

公开(公告)日：2016-07-28

申请号：US15090458

申请日：2016-04-04

申请人： GE HEALTHCARE BIO-SCIENCES AB

发明人： PER LOFVING ,  MATS OLSSON ,  SVEN WOUNDER ,  JERKER PERSSON

IPC分类号： B01D15/22

CPC分类号： B01D15/22 ,  B01D15/08 ,  B01D15/10 ,  B01D15/14 ,  B01D15/20 ,  G01N30/60 ,  G01N30/6004 ,  G01N30/6021 ,  G01N30/6047 ,  Y10T29/49819

摘要： A chromatography column having a longitudinal axis and comprising a column wall with a first end and a second end, a first end plate assembly removably connectable to said first end of the column wall, a second end plate assembly removably connectable to said second end of the column wall, wherein said first end plate assembly, said column wall and said second end plate assembly are arranged along the longitudinal axis of the column wherein the column wall, and/or first end plate assembly and/or second end plate assembly is/are rotatable about an axis of rotation wherein said axis of rotation is parallel to the longitudinal axis of said column and positioned outside the column.

公开(公告)号：US20160206972A1

公开(公告)日：2016-07-21

申请号：US15067694

申请日：2016-03-11

申请人： Bio-Rad Laboratories, Inc.

发明人： Sébastien Lefebvre ,  Lilian Portier ,  Maurice Agée

IPC分类号： B01D15/22 ,  B01D15/20

CPC分类号： B01D15/22 ,  B01D15/206 ,  B65B1/24 ,  F16L11/00 ,  F16L33/00 ,  G01N30/56 ,  G01N30/6021 ,  G01N30/603 ,  G01N30/6082 ,  G01N2030/565 ,  Y10T29/49865

摘要： A chromatography column design and a packing device, as well as a method for obtaining a column with a very compact and uniform bed are provided. The bed remains compact and uniform during operation of the column and during sanitization, storage and transportation of the column. The amount of hardware, particularly the hardware parts in contact with product, is minimized, especially with the implementation of an internal liner. With this internal liner, the packed bed can be separated from the column in intact form, or even prepared separately from, and outside of, the column.

公开(公告)号：US09110060B2

公开(公告)日：2015-08-18

申请号：US12251491

申请日：2008-10-15

申请人： Kristian Windahl

发明人： Kristian Windahl

IPC分类号： G01N30/56 ,  B01D15/20 ,  G01N30/60

CPC分类号： G01N30/56 ,  B01D15/206 ,  G01N30/6021 ,  G01N2030/565

摘要： A column packing system comprises a control unit provided with software for monitoring the column interior pressure and/or flow through the column for use in calculating the breakpoint where a movable adapter comes into contact with a consolidated bed of bed media. The calculated breakpoint is used by the control unit to determine how much further the movable adapter has to move into the column in order to achieve a desired amount of bed compression.

摘要翻译： 柱填充系统包括控制单元，该控制单元具有用于监测柱内部压力和/或流过柱的软件，用于计算可移动适配器与固定的床介质床接触的断点。 所计算的断点由控制单元用于确定可移动适配器进一步移动到柱中以进一步达到期望的床压缩量。