公开(公告)号：US20190078066A1

公开(公告)日：2019-03-14

申请号：US16101895

申请日：2018-08-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yan WANG

IPC: C12N9/12 ,  C07K14/00 ,  C12P19/34 ,  C07K19/00 ,  C12N9/96

Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.

公开(公告)号：US20190024039A1

公开(公告)日：2019-01-24

申请号：US16140171

申请日：2018-09-24

Applicant: AKONNI BIOSYSTEMS, INC. ,  BIO-RAD LABORATORIES, INC.

Inventor： Phillip BELGRADER ,  Benjamin HINDSON

IPC: C12N1/06 ,  C12N13/00 ,  C12Q1/6806 ,  C12N1/20

Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.

公开(公告)号：US20190018023A1

公开(公告)日：2019-01-17

申请号：US16145021

申请日：2018-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Roger Walker ,  Benedicte Jardin

IPC: G01N33/72 ,  G01N33/531 ,  C07K16/18 ,  G01N33/58

Abstract: Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided.

公开(公告)号：US20190002956A1

公开(公告)日：2019-01-03

申请号：US16022500

申请日：2018-06-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. STUMBO ,  George CARMAN ,  Steve HOBBS ,  Anthony J. MAKAREWICZ, JR. ,  Dmitri SIMONIAN ,  David GLADE ,  Joshua OEN ,  Denis PRISTINSKI ,  John DZENITIS

IPC: C12Q1/686 ,  B01L3/00 ,  G01N15/14 ,  B01F3/08 ,  C12Q1/6848 ,  C12Q1/6844

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US20180372680A1

公开(公告)日：2018-12-27

申请号：US16013743

申请日：2018-06-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yochanan URI ,  Igor KOCHETKOV ,  Uri ISSMAN ,  Shai AHARON

IPC: G01N27/447

Abstract: Gel cassette opening devices and methods for opening gel cassettes are provided.

公开(公告)号：US10161947B2

公开(公告)日：2018-12-25

申请号：US14702019

申请日：2015-05-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Christopher Yundt-Pacheco ,  Lakshmi Kuchipudi ,  Curtis Alan Parvin

IPC: G01N35/00 ,  G16H50/30 ,  G06F19/00

Abstract: Methods, apparatuses, and systems are disclosed for analyzing quality control (QC) strategies that are applied to testing processes an analyte in order to meet an acceptable level of probability of patient harm that could result from incorrect test results. The measure of patient harm takes into account severity of patient harm, as well as its occurrence. Methods include calculating, based on the parameters of the QC strategies and the test apparatus, an expected number of incorrect final results E(Nuf) due to a test system failure. The value of E(Nuf) can be used as part of a calculation of a predicted level of probability patient harm. The ratio of the acceptable level of probability of patient harm to the predicted level of probability patient harm can determine the adequacy of the QC strategies.

公开(公告)号：US20180363041A1

公开(公告)日：2018-12-20

申请号：US16013845

申请日：2018-06-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Dianna Maar

IPC: C12Q1/6848 ,  C12Q1/6832

Abstract: Methods and compositions for detecting genetic instability using digital amplification assays. The methods may be performed in a set of isolated volumes and generally may involve competitive hybridization of a competitor and a probe/primer with a normal allele and one or more mutant alleles of a microsatellite locus. The competitor may be configured to compete similarly with, or to outcompete, the primer/probe for hybridization with the normal allele. The primer/probe may be configured to outcompete the competitor for hybridization with various mutant alleles of the locus that alter the length of the repetitive sequence by different amounts. Isolated volumes in which the primer/probe outcompetes the competitor may be enumerated, and represent one or more of the mutant alleles. The methods may enable diagnosing microsatellite instability and treating a subject based on the diagnosis.

公开(公告)号：US10152519B2

公开(公告)日：2018-12-11

申请号：US15060260

申请日：2016-03-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Karl Nedwed ,  Ty Abshear

IPC: G06F17/30 ,  G06K9/52 ,  G06K9/62 ,  G06T11/20 ,  G01N21/35 ,  G06K9/46 ,  G01N21/552

Abstract: Systems, methods, and apparatuses are provided for identifying an optimal spectral match and potentially display the compared spectra. A sample spectrum of a sample substance can be compared to reference spectra to identify matches, thereby determining possibilities for what the sample substance is. Correction parameter(s) may be used for the sample spectrum and/or the reference spectrum. Initial value(s) for the correction parameter(s) can be applied to the sample spectrum and/or a reference spectrum, and a similarity score can be determined. The value(s) for the correction parameter(s) can be updated and iteratively improved to provide an optimal similarity score that satisfies a convergence criterion. Data about the reference substances having optimal similarity scores that are above a threshold can be output to a user, e.g., the reference spectra can overlay the sample spectrum. A user can then make a final determination of which reference substance corresponds to the sample substance.

公开(公告)号：US10131898B2

公开(公告)日：2018-11-20

申请号：US14805348

申请日：2015-07-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong

IPC: C12Q1/68 ,  C12P19/34 ,  C12N9/96 ,  C12N9/12 ,  C12Q1/6844

Abstract: Amplification reaction mixtures comprising a cationic surfactant and an anionic surfactant are provided.

公开(公告)号：US10119934B2

公开(公告)日：2018-11-06

申请号：US15272257

申请日：2016-09-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  B01D57/02 ,  C08F220/56

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.