公开(公告)号：US20240092104A1

公开(公告)日：2024-03-21

申请号：US17754866

申请日：2019-11-14

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Omer Gila ,  Napoleon J Leoni

IPC: B41J29/17 ,  B41J2/04 ,  B41J2/095

CPC classification number: B41J29/17 ,  B41J2/04 ,  B41J2/095 ,  B41J2002/043

Abstract: An image formation device includes a support, a fluid ejection device, and a first porous element. The support is to support movement of a substrate along a travel path, while the fluid ejection device is located along the travel path to deposit droplets of ink particles within a liquid carrier onto the substrate to at least partially form an image on the substrate. The first porous element is located downstream along the travel path from the fluid ejection device to be in contact against the substrate to remove, via electroosmotic flow through the first porous element, at least a portion of the liquid carrier from the substrate.

公开(公告)号：US11759793B2

公开(公告)日：2023-09-19

申请号：US17983479

申请日：2022-11-09

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Daixi Xin

IPC: B25J9/06 ,  B03C5/02 ,  B01D17/06 ,  B25J9/00 ,  B25J9/16

CPC classification number: B03C5/026 ,  B01D17/06 ,  B03C5/028 ,  B25J9/0087 ,  B25J9/06 ,  B25J9/1666

Abstract: An object separator may include a substrate, a fluid channel supported by the substrate, a pair of electrodes along the fluid channel to form a dielectrophoretic force to interact with an object entrained in a fluid, and an inertial pump supported by the substrate and positioned within the fluid channel to move the fluid along the fluid channel.

公开(公告)号：US20230287479A1

公开(公告)日：2023-09-14

申请号：US18016860

申请日：2020-07-21

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov

IPC: C12Q1/6823 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6823 ,  B01L3/502761 ,  B01L7/00 ,  B01L2200/0652 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2400/0487 ,  B01L2300/088 ,  B01L2300/1805

Abstract: An example method, consistent with the present disclosure, includes receiving in a microfluidic channel, a fluid sample and a reagent, where the reagent includes a reporter nucleic acid labeled with a detectable ligand. The method further includes identifying a target nucleic acid in the fluid sample using a guide ribonucleic acid (gRNA) and a programmable nuclease immobilized in a side channel fluidically coupled to the microfluidic channel. Responsive to identifying the target nucleic acid in the fluid sample, the method includes causing cleavage of the detectable ligand, and detecting the detectable ligand from the cleaved reporter nucleic acid in the side channel.

公开(公告)号：US20230251180A1

公开(公告)日：2023-08-10

申请号：US18012632

申请日：2020-07-08

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Fausto D'Apuzzo ,  Viktor Shkolnikov ,  Alexander N. Govyadinov

IPC: G01N15/14 ,  B01L3/00

CPC classification number: G01N15/1404 ,  G01N15/1434 ,  B01L3/502761 ,  G01N2015/1495 ,  B01L2300/0654 ,  B01L2200/0663 ,  B01L2400/086 ,  B01L2400/0487

Abstract: An example method for measuring deformability of a cell via a pressure field, consistent with the present disclosure, includes flowing a biologic sample containing a plurality of cells along a first fluidic channel and into an intersection between the first fluidic channel and a second fluidic channel of a microfluidic device. The method includes introducing a pressure field at the intersection and into the first fluidic channel via the second fluidic channel and a plurality of apertures in a channel wall disposed in the intersection. The method further includes measuring deformability of a cell among the plurality of cells responsive to the introduction of the pressure field.

公开(公告)号：US20230166264A1

公开(公告)日：2023-06-01

申请号：US18101513

申请日：2023-01-25

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Chien-Hua Chen

IPC: B01L7/00 ,  B01L3/00 ,  G01N33/543

CPC classification number: B01L7/52 ,  B01L3/502761 ,  G01N33/54386 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2300/1827 ,  B01L2400/0424 ,  B01L2400/086

Abstract: An example system includes an array of retaining features in a microfluidic cavity, an array of thermally controlled releasing features, and a controller coupled to each releasing feature in the array of releasing feature. Each retaining feature in the array of retaining features is to position capsules at a predetermined location, the capsules having a thermally degradable shell enclosing a biological reagent therein. Each releasing feature in the array of releasing features corresponds to a retaining feature and is to selectively cause degradation of the shell of a capsule. Each releasing feature is to generate thermal energy to facilitate degradation of the shell. The controller is to selectively activate at least one releasing feature in the array of thermally controlled releasing features to release the biological reagent in the capsules positioned at the retaining feature corresponding to the activated releasing feature.

公开(公告)号：US20230131266A1

公开(公告)日：2023-04-27

申请号：US17507152

申请日：2021-10-21

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov

IPC: C12N15/02 ,  C12M1/42 ,  C12M1/36 ,  C12M3/06

Abstract: A method of alignment and fusion of cells with an electrical field includes firing a first fluid dispenser of an electrofusion device until a first impedance sensor in a first microfluidic channel of the electrofusion device detects a presence of a first cell. The method includes firing a second fluid dispenser of the electrofusion device until a second impedance sensor in a second microfluidic channel of the electrofusion device detects a presence of a second cell. The method includes moving the first cell and the second cell into a merging chamber of the electrofusion device by firing a third fluid dispenser of the electrofusion device, in response to alignment of the first cell in the first microfluidic channel with the second cell in the second microfluidic channel. The method further includes fusing the first cell and the second cell in the merging chamber by creating an electrical field in the merging chamber.

公开(公告)号：US11583861B2

公开(公告)日：2023-02-21

申请号：US16605675

申请日：2017-10-30

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Michael W Cumbie ,  Chien-Hua Chen

IPC: B81B3/00 ,  G01N35/10 ,  B01L3/00

Abstract: In an example implementation, a reagent storage system for a microfluidic device includes a microfluidic chamber formed in a microfluidic device. A blister pack to store a reagent includes an electrically conductive membrane barrier adjacent to the chamber. A thinned region is formed in the membrane barrier, and a conductive trace is to supply electric current to heat and melt the thinned region. Melting the thinned region is to cause the membrane barrier to open and release the reagent into the chamber.

公开(公告)号：US20230014003A1

公开(公告)日：2023-01-19

申请号：US17778766

申请日：2019-12-20

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Fausto D'Apuzzo ,  Viktor Shkolnikov ,  Alexander N. Govyadinov

IPC: B41J2/14 ,  B41J2/165 ,  B41J2/21

Abstract: An integrated fluid ejection and imaging system may include a fluid ejector to eject a droplet of fluid onto a deposition site on a target, an imager to image the deposition site and a packaging supporting the fluid ejector and imager such that the fluid ejector and the imager are concurrently aimed at the deposition site on the target.

公开(公告)号：US11364496B2

公开(公告)日：2022-06-21

申请号：US16494160

申请日：2017-04-21

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Chien-Hua Chen ,  Michael W. Cumbie ,  Viktor Shkolnikov

IPC: B01L3/00

Abstract: An apparatus includes a polymer base layer having a surface. A die has a surface that is substantially coplanar with the surface of the polymer base layer. The die includes a fluidic actuator to control fluid flow across the surface of the die. A fluidic channel is coupled to the polymer base layer to provide a fluidic interconnect between the die and a fluidic input/output port.

公开(公告)号：US20220184610A1

公开(公告)日：2022-06-16

申请号：US17416565

申请日：2019-07-25

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Alexander Govyadinov ,  Viktor Shkolnikov ,  Diane R. Hammerstad

IPC: B01L3/00

Abstract: In example implementations, an apparatus is provided. The apparatus includes a channel, an energy source, and a transfection chamber. The channel includes an indentation to hold a cell. The energy source is to apply a shockwave to the cell in the channel to porate the cell. The transfection chamber is to store a reagent to be inserted into the cell after the cell is porated.