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    In situ production of furfural in a controlled amount in an alcohol production unit from a lignocellulosic biomass
    134.
    发明授权
    In situ production of furfural in a controlled amount in an alcohol production unit from a lignocellulosic biomass 有权
    在木质纤维素生物质的醇生产单元中原位生产受控量的糠醛

    公开(公告)号：US08980595B2

    公开(公告)日：2015-03-17

    申请号：US13320323

    申请日：2010-05-07

    申请人： Marcel Ropars ,  Caroline Aymard ,  Flora Ghisoni ,  Sandra Menir

    发明人： Marcel Ropars ,  Caroline Aymard ,  Flora Ghisoni ,  Sandra Menir

    IPC分类号： C12P7/02 ,  C12P7/16 ,  C12P1/00 ,  C12P7/10 ,  C12P17/04

    CPC分类号： C12P7/10 ,  C12P17/04 ,  C12P2201/00 ,  Y02E50/16 ,  Y02P20/582

    摘要： The present invention describes a process for the production of alcohol, known as second generation production, from a lignocellulosic substrate, in which a controlled quantity of furfural is produced in situ and which comprises at least the following steps: a first step for alkaline chemical pre-treatment of the substrate; a step for adjusting the pH of the substrate; then a step for enzymatic hydrolysis of said pre-treated substrate; a step for alcoholic fermentation of the hydrolysate obtained with an alcohol-producing microorganism; a step for separation/purification; and a step for acid conversion of at least one liquid fraction into furfural, said liquid fraction being obtained during one of the preceding steps; a step for mixing said furfural obtained with the pre-treated substrate during the pH adjusting step.

    摘要翻译： 本发明描述了一种从木质纤维素基质生产被称为第二代生产的方法，其中控制量的糠醛原位产生，其至少包括以下步骤：碱性化学预处理的第一步 底物的处理; 用于调节基材的pH的步骤; 然后是所述预处理底物的酶水解步骤; 用醇生产微生物获得的水解产物的酒精发酵步骤; 分离/纯化步骤 以及将至少一种液体馏分酸转化成糠醛的步骤，所述液体馏分在前述步骤之一中获得; 在pH调节步骤中混合所得糠醛与预处理底物的步骤。

    COMPOSITIONS AND METHODS FOR PRODUCING CHEMICALS AND DERIVATIVES THEREOF
    136.
    发明申请
    COMPOSITIONS AND METHODS FOR PRODUCING CHEMICALS AND DERIVATIVES THEREOF 有权
    用于生产化学品及其衍生物的组合物和方法

    公开(公告)号：US20140206047A1

    公开(公告)日：2014-07-24

    申请号：US14222453

    申请日：2014-03-21

    申请人： Synthetic Genomics, Inc.

    发明人： Spiros Kambourakis ,  Benjamin M. Griffin ,  Kevin V. Martin

    IPC分类号： C12P7/58 ,  C12P7/42 ,  C12P17/04

    CPC分类号： C12P7/58 ,  C07D307/33 ,  C07D309/30 ,  C12N15/52 ,  C12P7/42

    摘要： The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

    摘要翻译： 本发明提供了生产一种或多种酶途径的产物的方法。 用于本发明方法的途径包括一个或多个转化步骤，例如将古罗糖醛酸酶促转化成D-葡糖二酸（步骤7）; 将5-酮葡萄糖酸（5-KGA）酶促转化成L-阿魏酸（步骤15）; 步骤7b）中将L-阿魏酸转化成Idaric酸的酶促转化; 并将5-酮基葡萄糖酸酯酶促转化成4,6-二羟基2,5-二酮己酸酯（2,5-DDH）（步骤16）。 在一些实施方案中，本发明的方法产生作为产物的2,5-呋喃二羧酸（FDCA）。 所述方法包括作为步骤的酶和化学转化。 还提供了将葡萄糖转化为5-脱氢-4-脱氧葡糖二酸（DDG）和将葡萄糖转化为2,5-呋喃二羧酸（FDCA）的各种途径。 该方法还涉及使用以高特异性和高效率进行反应的工程化酶。 可以生产的其它产品包括代谢产物，例如但不限于古洛糖醛酸，L-艾杜糖醛酸，id酸，葡糖二酸。 可以使用葡萄糖作为底物或在本发明的任何方法或途径中使用任何中间体来生产任何产物。

    BIOMASS LIQUEFACTION PROCESSES, AND USES OF SAME
    138.
    发明申请
    BIOMASS LIQUEFACTION PROCESSES, AND USES OF SAME 有权
    生物量液化过程及其用途

    公开(公告)号：US20130330788A1

    公开(公告)日：2013-12-12

    申请号：US13754352

    申请日：2013-01-30

    申请人： Purdue Research Foundation

    发明人： Michael R. Ladisch ,  Nathan Mosier ,  Youngmi Kim

    IPC分类号： C12P7/10 ,  C12P17/04

    CPC分类号： C12P7/14 ,  C12N1/22 ,  C12P7/10 ,  C12P17/04 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12Y302/01004 ,  Y02E50/16

    摘要： Described are processes for the liquefaction of lignocellulosic biomass under the digestive action of dicarboxylic acid(s). Such digests can exhibit enhanced flowability, reduced volume, and significant biomass conversion to dissolved components, and can in some embodiments be further liquefied by contact with an enzyme. Products resultant of these steps can be used for their sugar content to manufacture biofuels or other products.

    摘要翻译： 描述了在二羧酸的消化作用下液化木质纤维素生物质的方法。 这样的消化可以表现出增强的流动性，减小的体积和显着的生物质转化成溶解的组分，并且在一些实施方案中可以通过与酶的接触进一步液化。 这些步骤的产物可用于其糖含量以制造生物燃料或其它产品。