公开(公告)号：US10829811B2

公开(公告)日：2020-11-10

申请号：US15965595

申请日：2018-04-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12N15/10 ,  C12Q1/6886 ,  C12Q1/6874

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US10829787B2

公开(公告)日：2020-11-10

申请号：US15294586

申请日：2016-10-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xin Yu ,  Xiquan Liang ,  Xavier de Mollerat du Jeu ,  Robert Jason Potter

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/88 ,  A61K48/00

Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

公开(公告)号：US10821444B2

公开(公告)日：2020-11-03

申请号：US15457633

申请日：2017-03-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald Danehy ,  Jorge Fonseca ,  Jayanthi Prasad

IPC: G01N35/10 ,  B01L3/00 ,  B01L3/02

Abstract: A system for preparing biological sample contains a body including a proximal side and a distal side, a plurality of mandrels, a plurality of resilient elements, a plurality of fluid dispensers, and one or more samples. The mandrels are moveably positioned within the body, where each resilient element engages a respective one of the mandrels. Each of the fluid dispensers is configured to engage a distal end of a corresponding one of the mandrels. Each sample comprises a solution containing one or more nucleic acid sequences contained within at least one of the fluid dispensers.

公开(公告)号：US10821197B2

公开(公告)日：2020-11-03

申请号：US16233403

申请日：2018-12-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael E. Goodwin ,  Nephi D. Jones ,  Bradley H. Buchanan

IPC: A61L2/26 ,  A61L2/08 ,  A61L2/00

Abstract: A container includes a first wall and an opposing second wall that extend between a first end and an opposing second end, a first fluid flow path being bounded between the first wall and the second wall. An inlet is formed at the first end and communicates with the first fluid flow path. An outlet is formed at the second end and communicates with the first fluid flow path. The first wall includes a primary wall portion having a first opening extending therethrough in alignment with the first fluid flow path. A secondary wall portion extends over the first opening and is secured to the primary wall portion so as to seal the first opening closed, the secondary wall portion being comprised of a film having a thickness that is less than a thickness of the primary wall portion.

公开(公告)号：US20200333291A1

公开(公告)日：2020-10-22

申请号：US16922754

申请日：2020-07-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ian WALTON ,  Robert COBENE ,  Alexander DUKHOVNY ,  Adam SANNICANDRO ,  John DIXON ,  Achim KARGER ,  Dan KLINE

IPC: G01N27/447 ,  G01N35/10

Abstract: In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.

公开(公告)号：US20200332343A1

公开(公告)日：2020-10-22

申请号：US16866211

申请日：2020-05-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG

IPC: C12Q1/686 ,  C07D207/16 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C07D211/60 ,  C11D1/88 ,  C12N9/12 ,  C12N9/96

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

公开(公告)号：USD899440S1

公开(公告)日：2020-10-20

申请号：US29669666

申请日：2018-11-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Matthew Schifano ,  Brian Johnson ,  Justin Wilson ,  Austin Beer ,  Naomi Tirronen ,  Magdalena Wielopolski ,  Aron Wahl ,  Matthew Murray

公开(公告)号：USD899434S1

公开(公告)日：2020-10-20

申请号：US29637491

申请日：2018-02-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Sean Zimmerman ,  Scott Rickes ,  Jason Dallwig ,  Kathleen Free ,  Joseph Lee ,  Jennifer Hedlind

公开(公告)号：US20200326240A1

公开(公告)日：2020-10-15

申请号：US16715057

申请日：2019-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael E. Goodwin

IPC: G01K1/14 ,  C12M1/00 ,  C12M3/00 ,  C12M1/06 ,  C12M1/26 ,  C12M1/34 ,  G01N1/10 ,  C12M1/28

Abstract: A container system includes a flexible bag, a probe port, and a probe. The probe port includes an elongated tubular member having an interior surface bounding a first passageway extending between a first end and an opposing second end, the tubular member being made of a polymeric or elastomeric material and being flexible. The probe port also includes a flange coupled to the tubular member and radially outwardly projecting therefrom, the flange being secured to the flexible bag so that the first end of the tubular member projects into a chamber of the flexible bag while the second end of the first passageway of the tubular member is accessible from outside of the flexible bag. The probe can be received within the first passageway of the probe port.

公开(公告)号：US20200325514A1

公开(公告)日：2020-10-15

申请号：US16736258

申请日：2020-01-07

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Todd PETERSON ,  Axel TREFZER ,  Thomas POEHMERER ,  Federico KATZEN ,  Xiquan LIANG

IPC: C12P19/34 ,  C12N15/10

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).