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    COMPOSITIONS
    11.
    发明申请
    COMPOSITIONS 审中-公开
    组合物

    公开(公告)号：US20100184059A1

    公开(公告)日：2010-07-22

    申请号：US12664175

    申请日：2008-06-16

    申请人： Martin Alan Lee ,  Jennifer Mitchell ,  Gerald Adams ,  Valerie Adams

    发明人： Martin Alan Lee ,  Jennifer Mitchell ,  Gerald Adams ,  Valerie Adams

    IPC分类号： C12Q1/68 ,  G01N21/76

    CPC分类号： C12N9/96

    摘要： A composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried form and comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessary for conducting said chemical or biochemical reaction, including at least one reagent which is fluorescent (ii) a glass forming agent, and (iii) threonine. Kits comprising these compositions and methods of using them form a further aspect of the invention.

    摘要翻译： 一种用于进行化学或生物化学反应的组合物，所述组合物为冷冻干燥形式，并且包含（i）一组试剂，其包含进行所述化学或生物化学反应所需的至少一些化学或生物化学试剂，包括在 至少一种荧光试剂（ii）玻璃形成剂，和（iii）苏氨酸。 包含这些组合物的组合物和使用它们的方法形成本发明的另一方面。

    Fluorescence-Based Detection Methods and Apparatus
    14.
    发明申请
    Fluorescence-Based Detection Methods and Apparatus 失效
    基于荧光的检测方法和装置

    公开(公告)号：US20080290292A1

    公开(公告)日：2008-11-27

    申请号：US12095444

    申请日：2006-12-11

    申请人： David James Squirrell ,  Martin Alan Lee

    发明人： David James Squirrell ,  Martin Alan Lee

    IPC分类号： G01J1/58

    CPC分类号： G01N21/6428 ,  G01N21/645 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N2201/0691

    摘要： In apparatus for detecting the emission of fluorescent radiation from a sample in response to excitation by two or more different radiation sources (145, 160) over the same time period, each radiation source (145, 160) is imprinted with a modulation regime which can then be separately detected in the emitted fluorescent radiation. For instance in techniques based on fluorescent resonant energy transfer (“FRET”), the drive current to two or more different LED sources (145, 160) may be frequency modulated, or pulse width modulated, according to different modulation regimes. Responses of the sample, for instance of different donor/acceptor probes contained in the sample, to each of the sources (145, 160) can then be separately detected by means of the different modulation regimes, even where the wavelengths or wavelength ranges of the responses are the same or overlapping.

    摘要翻译： 在用于响应于两个或更多个不同的辐射源（145,160）在相同时间段的激发来检测来自样品的荧光辐射的发射的装置中，每个辐射源（145,160）被印刷有调制方式，其可以 然后在发射的荧光辐射中单独检测。 例如，在基于荧光共振能量转移（“FRET”）的技术中，可以根据不同的调制方式对两个或更多个不同的LED源（145,160）的驱动电流进行频率调制或脉冲宽度调制。 然后可以通过不同的调制方式单独地检测样品（例如包含在样品中的不同施主/受体探针）到每个源（145,160）的响应，即使其中波长或波长范围 回应是相同或重叠的。

    VESSEL AND PROCESS FOR PRODUCTION THEREOF
    16.
    发明申请
    VESSEL AND PROCESS FOR PRODUCTION THEREOF 有权
    船舶及其生产方法

    公开(公告)号：US20140008376A1

    公开(公告)日：2014-01-09

    申请号：US13814545

    申请日：2011-08-02

    申请人： David Whitaker ,  Kevin Neale ,  David Squirrell

    发明人： David Whitaker ,  Kevin Neale ,  David Squirrell

    IPC分类号： B65D1/40 ,  B29D22/00

    CPC分类号： B65D1/40 ,  B01L3/508 ,  B01L3/5082 ,  B01L3/52 ,  B01L2200/12 ,  B01L2300/0858 ,  B01L2300/123 ,  B29D22/003

    摘要： A vessel of a malleable or mouldable material having one or more side walls and a closed base integral with said one or more side walls, an opening in the top of the vessel, and further comprising two or more projections extending into the interior of the vessel generally laterally from said side walls, wherein said projections are also integral with said side wall. Vessels of this type are produced by a process in which ridges are deformed into projections within a vessel blank, and this process forms a further aspect of the invention. They are particularly useful for holding solid masses such as small beads or cakes in position in the vessel.

    摘要翻译： 具有一个或多个侧壁和与所述一个或多个侧壁成一体的封闭基座的可延展或可模制材料的容器，容器顶部中的开口，并且还包括延伸到容器内部的两个或更多突起 大致横向地从所述侧壁，其中所述突起也与所述侧壁成一体。 这种类型的容器是通过一种方法产生的，其中脊变形成容器坯料内的突起，并且该方法形成了本发明的另一方面。 它们特别可用于将诸如小珠或蛋糕的固体块保持在容器中的适当位置。

    SEQUENCE DETECTION ASSAY
    17.
    发明申请
    SEQUENCE DETECTION ASSAY 审中-公开
    序列检测测定

    公开(公告)号：US20130210001A1

    公开(公告)日：2013-08-15

    申请号：US13634925

    申请日：2011-03-15

    申请人： Martin Lee

    发明人： Martin Lee

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q2565/101 ,  C12Q2527/107

    摘要： There is provided a method of detecting the presence in a sample of a first target sequence and a second target sequence within a test region of a nucleic acid sequence comprising conducting a nucleic acid amplification reaction, to form a forward amplicon strand and a reverse amplicon strand of the test region, contacting the forward amplicon strand with a first probe labelled with a first FRET label and capable of hybridising to the first target sequence of complement thereof in the forward amplicon strand, and contacting the reverse amplicon strand with a second probe labelled with a second FRET label and capable of hybridising to the second target sequence or complement thereof in the reverse amplicon strand; wherein the nucleic acid amplification reaction is conducted using a forward amplification primer labelled with a third FRET label and a reverse amplification primer labelled with a fourth FRET label, the forward primer being incorporated into the forward amplicon strand and the second primer being incorporated into the reverse amplicon strand during the amplification reaction; and further wherein the first and third FRET labels form a FRET pair and the second and fourth FRET labels form a different FRET pair, each FRET pair comprising a donor label; the method further comprising the steps of exposing the sample to an excitation source having a wavelength which excites the donor label in the first FRET pair and the donor label in the second FRET pair, detecting fluorescence from the sample and relating this to the presence or absence of the first and second target sequences.

    摘要翻译： 提供了一种检测在核酸序列的测试区域内的第一靶序列和第二靶序列在样品中的存在的方法，包括进行核酸扩增反应，形成正向扩增子链和反向扩增子链 使前向扩增子链与标记有第一FRET标记的第一探针接触，并且能够与正向扩增子链中的其互补序列的第一个靶序列杂交，并将反向扩增子链与第二个探针标记 第二FRET标记并能够与反向扩增子链中的第二个靶序列或其互补序列杂交; 其中所述核酸扩增反应使用用第三FRET标记标记的正向扩增引物和用第四FRET标记标记的反向扩增引物进行，所述正向引物并入正向扩增子链中，第二引物并入反向 在扩增反应期间扩增子链; 并且其中所述第一和第三FRET标签形成FRET对，并且所述第二和第四FRET标签形成不同的FRET对，每个FRET对包括供体标签; 该方法还包括以下步骤：将样品暴露于激发源，该激发源具有激发第一FRET对中的供体标记和第二FRET对中的供体标记的波长，检测来自样品的荧光并将其与存在或不存在 的第一和第二靶序列。

    Analytical Method and Kit
    20.
    发明申请
    Analytical Method and Kit 失效
    分析方法和试剂盒

    公开(公告)号：US20080233588A1

    公开(公告)日：2008-09-25

    申请号：US12064103

    申请日：2006-08-08

    申请人： David James Squirrell

    发明人： David James Squirrell

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/6816 ,  C12Q1/6872 ,  C12Q2563/103 ,  C12Q2537/143 ,  C12Q2525/117

    摘要： Analytical methods using RNA-containing probes for the detection or analysis of nucleic acid sequences is described. These probes are contacted with a sample suspected of containing the nucleic acid sequence and if they form duplexes, they are hydrolysed. This may be done, for example during an amplification reaction. AMP generated as a result of the hydrolysis is converted to ATP. The ATP may then be detected using bioluminescent reagents. Inclusion of modified adenosine in at least one probe means that the signal arising from one probe will give rise to a different and distinguishable bioluminescent signal thus enabling the use of for example an internal control in bioluminescently-reported nucleic acid tests.

    摘要翻译： 描述了使用含RNA探针进行核酸序列检测或分析的分析方法。 这些探针与怀疑含有核酸序列的样品接触，如果它们形成双链体，则它们被水解。 这可以例如在扩增反应期间完成。 作为水解产生的AMP被转化为ATP。 然后可以使用生物发光试剂检测ATP。 在至少一个探针中包含修饰的腺苷意味着由一个探针产生的信号将产生不同的且可区分的生物发光信号，从而使得能够在生物发光报道的核酸测试中使用例如内部对照。