利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

36 条记录 (耗时 0.025 秒)

    Detection system
    3.
    发明授权
    Detection system 失效
    检测系统

    公开(公告)号：US07700275B2

    公开(公告)日：2010-04-20

    申请号：US10478788

    申请日：2002-05-24

    申请人： Martin Alan Lee

    发明人： Martin Alan Lee

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6851 ,  C12Q2565/101 ,  C12Q2561/113

    摘要： A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising: performing nucleic acid amplification on the sample in the presence of (a) a DNA duplex binding agent, (b) a nucleic acid polymerase and (c) a reagent comprising an amplification primer which can hybridize to said target sequence when in single stranded form and which is connected at its 5′ end to a probe which carries a label by way of a chemical linking group, said labeled probe being of a sequence which is similar to that of the said target nucleic acid sequence, such that it can hybridize to a complementary region in an amplification product, and wherein the label is able to absorb fluorescence from or donate fluorescent energy to the DNA duplex binding agent; and monitoring fluorescence of said sample.

    摘要翻译： 一种用于检测样品中靶核酸序列的存在的方法，所述方法包括：在（a）DNA双链体结合剂，（b）核酸聚合酶和（c ）包含扩增引物的试剂，所述扩增引物可以单链形式与所述靶序列杂交，并且其在其5'端与通过化学连接基团携带标记的探针连接，所述标记的探针是序列 其与所述靶核酸序列类似，使得其可以与扩增产物中的互补区杂交，并且其中所述标记能够从所述DNA双链体结合剂吸收荧光或向其中提供荧光能; 并监测所述样品的荧光。

    Reaction system for thermal cycling
    4.
    发明授权
    Reaction system for thermal cycling 失效
    热循环反应系统

    公开(公告)号：US07537927B2

    公开(公告)日：2009-05-26

    申请号：US11782255

    申请日：2007-07-24

    申请人： David James Squirrell ,  Martin Alan Lee

    发明人： David James Squirrell ,  Martin Alan Lee

    IPC分类号： C12M1/00 ,  C12M3/00

    CPC分类号： B01L7/52

    摘要： Method and apparatus for carrying out a thermal cycling reaction, wherein a succession of samples is conveyed through a series of sequentially arranged temperature control sites, each of the sites comprising means for supplying an electric current to, or inducing an electric current in, sample-containing vessels passing through it so as to induce temperature changes in the samples. Also provided is a sample support and its production, the support comprising a succession of sample vessels arranged sequentially one behind the next, preferably in the form of a linked chain, the support comprising an electrically conducting, preferably plastics, material which heats when an electric current passes through it.

    摘要翻译： 用于进行热循环反应的方法和装置，其中一系列样品被传送通过一系列顺序排列的温度控制位点，每个位置包括用于向样品中提供电流或引起电流的装置， 通过其容纳的容器以引起样品中的温度变化。 还提供了一种样品支持体及其生产，该支撑体包括一系列连续的样品容器，这些样品容器顺序地布置在下一个之后，优选地以连接的链条的形式，该支撑体包括导电的，优选塑料的材料，其在电 电流通过它。

    Method For Detecting Viable Cells In A Sample By Using A Virus
    5.
    发明申请
    Method For Detecting Viable Cells In A Sample By Using A Virus 有权
    通过使用病毒检测样品中活细胞的方法

    公开(公告)号：US20080166700A1

    公开(公告)日：2008-07-10

    申请号：US11816331

    申请日：2006-02-16

    申请人： David James Squirrell ,  Martin Alan Lee

    发明人： David James Squirrell ,  Martin Alan Lee

    IPC分类号： C12Q1/70 ,  C12N7/00

    CPC分类号： C12Q1/689 ,  C12Q1/70

    摘要： A method for detecting viable cells such as bacterial cells, within a sample, said method comprising (i) incubating said sample with a virus which is able to infect said cells under conditions which allow said virus to infect and replicate within any such cells which are viable; (ii) detecting any nucleic acid obtained by replication of the virus in said cell.

    摘要翻译： 一种用于检测样品内的活细胞如细菌细胞的方法，所述方法包括（i）在允许所述病毒在任何这样的细胞内感染和复制的条件下，使能够感染所述细胞的病毒孵育所述样品，所述病毒是 可行的 （ii）检测通过在所述细胞中复制病毒获得的任何核酸。

    Reaction system for thermal cycling
    6.
    发明授权
    Reaction system for thermal cycling 失效
    热循环反应系统

    公开(公告)号：US07264961B2

    公开(公告)日：2007-09-04

    申请号：US10220970

    申请日：2001-03-07

    申请人： David James Squirrell ,  Martin Alan Lee

    发明人： David James Squirrell ,  Martin Alan Lee

    IPC分类号： C12M1/36

    CPC分类号： B01L7/52

    摘要： Method and apparatus for carrying out a thermal cycling reaction, wherein a succession of samples is conveyed through a series of sequentially arranged temperature control sites, each of the sites comprising means for supplying an electric current to, or inducing an electric current in, sample-containing vessels passing through it so as to induce temperature changes in the samples. Also provided is a sample support and its production, the support comprising a succession of sample vessels arranged sequentially one behind the next, preferably in the form of a linked chain, the support comprising an electrically conducting, preferably plastics, material which beats when an electric current passes through it.

    摘要翻译： 用于进行热循环反应的方法和装置，其中一系列样品被传送通过一系列顺序排列的温度控制位点，每个位置包括用于向样品中提供电流或引起电流的装置， 通过其容纳的容器以引起样品中的温度变化。 还提供了样品支持体及其生产，该支撑体包括一系列连续的样品容器，其顺次地排列在下一个之后，优选地以连接的链条的形式，所述支撑件包括导电的，优选塑料的材料，当电 电流通过它。

    Reaction system for performing in the amplification of nucleic acids
    7.
    发明授权
    Reaction system for performing in the amplification of nucleic acids 有权
    用于在核酸扩增中进行的反应体系

    公开(公告)号：US07264950B1

    公开(公告)日：2007-09-04

    申请号：US10089498

    申请日：2000-09-29

    申请人： Martin Alan Lee ,  Hilary Bird ,  Dario Lyall Leslie ,  David James Squirrell ,  John Shaw ,  David Wenn ,  Julie Deacon

    发明人： Martin Alan Lee ,  Hilary Bird ,  Dario Lyall Leslie ,  David James Squirrell ,  John Shaw ,  David Wenn ,  Julie Deacon

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： B01L7/52 ,  B01L3/52 ,  B01L3/523

    摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.

    摘要翻译： 一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶 反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。 描述了用于实现该方法的装置以及用于该方法的一次性单元。 该方法特别适用于快速PCR反应。

    FREEZE-DRIED COMPOSITIONS FOR CARRYING OUT PCR AND OTHER BIOCHEMICAL REACTIONS
    8.
    发明申请
    FREEZE-DRIED COMPOSITIONS FOR CARRYING OUT PCR AND OTHER BIOCHEMICAL REACTIONS 审中-公开
    用于携带PCR和其他生物化学反应的冻干组合物

    公开(公告)号：US20110159497A1

    公开(公告)日：2011-06-30

    申请号：US12664183

    申请日：2008-06-16

    申请人： Martin Alan Lee ,  Jennifer Mitchell ,  Emma Henderson

    发明人： Martin Alan Lee ,  Jennifer Mitchell ,  Emma Henderson

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12Q2527/125

    摘要： A composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried form and comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessary for conducting said chemical or biochemical reaction, (ii) a glass forming agent, (iii) a stabilising agent therefore and (iv) fish gelatine. In particular compositions for carrying out PCR are foreseen. Kits comprising these compositions and methods for using them form a further aspect of the invention.

    摘要翻译： 一种用于进行化学或生物化学反应的组合物，所述组合物为冷冻干燥形式，并且包含（i）一组试剂，其包含进行所述化学或生物化学反应所需的至少一些化学或生物化学试剂，（ii） ），玻璃形成剂，（iii）稳定剂，和（iv）鱼明胶。 特别地，预期用于进行PCR的组合物。 包含这些组合物的组合物和使用它们的方法形成本发明的另一方面。

    COMPOSITIONS
    9.
    发明申请
    COMPOSITIONS 审中-公开
    组合物

    公开(公告)号：US20100184059A1

    公开(公告)日：2010-07-22

    申请号：US12664175

    申请日：2008-06-16

    申请人： Martin Alan Lee ,  Jennifer Mitchell ,  Gerald Adams ,  Valerie Adams

    发明人： Martin Alan Lee ,  Jennifer Mitchell ,  Gerald Adams ,  Valerie Adams

    IPC分类号： C12Q1/68 ,  G01N21/76

    CPC分类号： C12N9/96

    摘要： A composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried form and comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessary for conducting said chemical or biochemical reaction, including at least one reagent which is fluorescent (ii) a glass forming agent, and (iii) threonine. Kits comprising these compositions and methods of using them form a further aspect of the invention.

    摘要翻译： 一种用于进行化学或生物化学反应的组合物，所述组合物为冷冻干燥形式，并且包含（i）一组试剂，其包含进行所述化学或生物化学反应所需的至少一些化学或生物化学试剂，包括在 至少一种荧光试剂（ii）玻璃形成剂，和（iii）苏氨酸。 包含这些组合物的组合物和使用它们的方法形成本发明的另一方面。