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公开(公告)号：US5476791A
公开(公告)日：1995-12-19
申请号：US243521
申请日：1994-05-16
申请人： Akihiro Sakimae , Yuri Kagawa , Ryozo Numazawa , Hisao Onishi
发明人： Akihiro Sakimae , Yuri Kagawa , Ryozo Numazawa , Hisao Onishi
IPC分类号： C07D207/16 , C07D277/06 , C12P7/40 , C12P41/00 , C12P9/00
CPC分类号： C12P7/40 , C07D207/16 , C07D277/06 , C12P41/005 , Y10S435/822
摘要： N-(3-acetylthio-2-D-methylpropionyl)-L-proline useful as a precursor of captopril, one of antihypertensive agents, is prepared by allowing thioacetic acid to react with methyl methacrylate to prepare racetalc methyl 3-acetylthio-2-DL-methylpropionate, contacting the propionate with cells of, for example, Pseudomonas in order to effect biological and asymmetric hydrolysis, extracting 3-acetylthio-D-2-methylpropionic acid and allowing the acid to react with L-proline.
摘要翻译： 通过使硫代乙酸与甲基丙烯酸甲酯反应制备N-乙酰基-3-乙酰硫基-2-脯氨酸，其是抗高血压药之一，可用作卡托普利前体的N-（3-乙酰硫基-2-D-甲基丙酰基）-L-脯氨酸， DL-甲基丙酸酯，使丙酸酯与例如假单胞菌的细胞接触以进行生物和不对称水解，提取3-乙酰硫基-D-2-甲基丙酸，并使酸与L-脯氨酸反应。
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公开(公告)号：US5473081A
公开(公告)日：1995-12-05
申请号：US181168
申请日：1994-01-13
IPC分类号： C07D207/16 , C07D207/12
CPC分类号： C07D207/16
摘要： An L-proline derivative represented by: ##STR1## wherein R.sub.2 represents a lower alkyl group is prepared by reacting L-proline with a D-.alpha.-alkyl-.beta.-acylthiopropionic acid halide, ##STR2## wherein R.sub.1 represents an acyl group, X represents a halogen atom and R.sub.2 has the same meaning as defined above. The halide is added to an aqueous medium containing L-proline and a condensation agent for deacidification. The halide and L-proline are reacted while the reaction temperature and pH are maintained at 12.degree. C. or lower and within a range of 10.5-11.5, whereby a compound represented by: ##STR3## wherein R.sub.1 and R.sub.2 have the same meanings as defined above is obtained. The resulting compound is then subjected to deacylation under strong alkaline conditions without being isolated from the reaction mixture, thereby yielding the target L-proline derivative.
摘要翻译： 由下式表示的L-脯氨酸衍生物：其中R2表示低级烷基，其通过L-脯氨酸与D-α-烷基-β-酰基硫代丙酰卤反应来制备，其中R1表示酰基，X 表示卤素原子，R2表示与上述相同的含义。 将卤化物加入到含有L-脯氨酸和用于脱酸的缩合剂的水性介质中。 使卤化物和L-脯氨酸反应，同时将反应温度和pH保持在12℃或更低并且在10.5-11.5的范围内，由此得到由下式表示的化合物：其中R1和R2具有相同的含义 获得上述定义。 然后将所得化合物在强碱性条件下进行脱酰基，而不从反应混合物中分离，从而得到目标L-脯氨酸衍生物。
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公开(公告)号：US4885244A
公开(公告)日：1989-12-05
申请号：US104219
申请日：1987-10-05
申请人： Takao Miyamori , Ryozo Numazawa , Akihiro Sakimae , Hisao Onishi
发明人： Takao Miyamori , Ryozo Numazawa , Akihiro Sakimae , Hisao Onishi
CPC分类号： C08B37/0072 , C12P19/26 , Y10T436/188
摘要： High molecular weight hyaluronic acid can be efficiently produced by incorporating one or more compounds having at least one aromatic ring to which one or more hydroxyl groups are attached into a medium for producing hyaluronic acid by a bacterium capable of producing hyaluronic acid. By using this method, a sufficient amount of hyaluronic acid can be easily obtained without decomposing the high molecular structure of hyaluronic acid so that the process for isolating and purifying hyaluronic acid from the medium can be simplified.
摘要翻译： 高分子量透明质酸可以通过将一种或多种具有至少一个芳环的化合物（其中一个或多个羟基被连接到用于产生透明质酸的细菌的培养基中）而被能够产生透明质酸的细菌产生。 通过使用该方法，可以容易地获得足够量的透明质酸，而不会分解透明质酸的高分子结构，从而可以简化从培养基中分离和纯化透明质酸的方法。
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公开(公告)号：US4276381A
公开(公告)日：1981-06-30
申请号：US60907
申请日：1979-07-26
申请人： Akihiro Sakimae , Hisao Onishi
发明人： Akihiro Sakimae , Hisao Onishi
摘要： Immobilized enzymes or microorganisms are prepared by dispersing lumps of ice containing an enzyme or microorganism in an organic solvent having a water-insoluble high-molecular weight substance dissolved therein, and then removing the organic solvent to entrap the ice lumps in the water-insoluble high-molecular weight substance. Deactivation of the enzyme or microorganism by the organic solvent is prevented by the enzyme or microorganism being entrapped in the lumps of ice.
摘要翻译： 固定的酶或微生物通过将含有酶或微生物的冰块块溶解在其中溶解有不溶于水的高分子量物质的有机溶剂中而制备，然后除去有机溶剂以将冰块截留在不溶于水的高温 分子量物质。 通过酶或微生物被捕获在冰块中来防止酶或微生物被有机溶剂的失活。
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