公开(公告)号：US20070164003A1

公开(公告)日：2007-07-19

申请号：US10593771

申请日：2005-03-25

申请人： Yuzuru Takamura ,  Akiko Iiduka ,  Eiichi Tamiya

发明人： Yuzuru Takamura ,  Akiko Iiduka ,  Eiichi Tamiya

IPC分类号： B23K9/00 ,  B23K9/02

CPC分类号： G01N21/68 ,  G01N21/67 ,  G01N21/69

摘要： A method for generating plasma and a method for elemental analysis, each comprising the steps of providing a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; filling the flow channel and the narrow portion with a conductive liquid, and thereafter applying an electric field to the narrow portion, to conduct the electric field through the narrow portion, thereby generating plasma at the narrow portion. An apparatus for generating plasma, the apparatus for generating plasma comprising a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; and a means of applying an electric field to the narrow portion to conduct the electric field through the narrow portion; and an apparatus for emission spectroscopic analysis comprising the apparatus for generating plasma as defined above.

摘要翻译： 一种用于产生等离子体的方法和元素分析方法，每个方法包括以下步骤：在由绝缘材料制成的流动通道中提供窄部分，所述窄部分的横截面积明显小于所述绝缘材料的横截面面积 流通道 用导电液体填充流路和狭窄部分，然后向狭窄部分施加电场，使电场通过狭窄部分，从而在狭窄部分产生等离子体。 一种用于产生等离子体的装置，所述用于产生等离子体的装置包括由绝缘材料制成的流动通道中的窄部分，所述窄部分具有明显小于所述流动通道的横截面积的横截面面积; 以及向狭窄部分施加电场以通过所述狭窄部分传导电场的装置; 以及用于发射光谱分析的装置，其包括如上所述产生等离子体的装置。

公开(公告)号：US06200793B1

公开(公告)日：2001-03-13

申请号：US09117245

申请日：1998-07-27

申请人： A. K. M. Quamrul Hasan ,  Eiichi Tamiya

发明人： A. K. M. Quamrul Hasan ,  Eiichi Tamiya

IPC分类号： C12N952

CPC分类号： A23J3/341 ,  C12N9/52 ,  Y10S435/85

摘要： A psychrophilic protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, hemoglobin and albumin to specifically decompose them, and the substrate specificity decreases in the order of casein, gelatin, hemoglobin and albumin; (b) optimal pH: 8.0; (c) pH stability: the protease is stable in the range of pH 6.5 to pH 10.0 at 30° C. for 1 hour; (d) optimal temperature: about 40° C.; (e) temperature stability: at pH 7 for 1 hour, the protease is active at a temperature up to 30° C., but it is inactivated at 40° C. as much as about 40% and completely inactivated at 50° C. in about 10 minutes; (f) enzyme activity: the protease has 50% or more of its maximum activity at about 20° C.; (g) the active center of the enzyme is serine; and (h) the molecular weight of the protease is about 70 kDa as measured by SDS-PAGE.

摘要翻译： 这里公开了一种嗜热蛋白酶，其具有以下物理化学性质：（a）比活性和底物特异性：蛋白酶作用于酪蛋白，明胶，血红蛋白和白蛋白以特异性分解它们，底物特异性按酪蛋白，明胶的顺序降低 ，血红蛋白和白蛋白; （b）最佳pH：8.0; （c）pH稳定性：蛋白酶在30℃下在pH 6.5至pH 10.0范围内稳定1小时; （d）最佳温度：约40℃。 （e）温度稳定性：在pH7下1小时，蛋白酶在高达30℃的温度下是活性的，但在40℃下灭活达40％左右，并在50℃完全失活。 约10分钟; （f）酶活性：蛋白酶在约20℃下具有50％以上的最大活性; （g）酶的活性中心是丝氨酸; 和（h）通过SDS-PAGE测量，蛋白酶的分子量为约70kDa。

公开(公告)号：US08664003B2

公开(公告)日：2014-03-04

申请号：US13569291

申请日：2012-08-08

申请人： Eiichi Tamiya ,  Yoshiyuki Yokoyama ,  Satoshi Fujiki ,  Katsumi Tanino ,  Atsushi Muraguchi ,  Hiroyuki Kishi ,  Yoshiharu Tokimitsu ,  Shohei Yamamura

发明人： Eiichi Tamiya ,  Yoshiyuki Yokoyama ,  Satoshi Fujiki ,  Katsumi Tanino ,  Atsushi Muraguchi ,  Hiroyuki Kishi ,  Yoshiharu Tokimitsu ,  Shohei Yamamura

IPC分类号： C12Q1/24

CPC分类号： C12N5/0068 ,  C12N2533/12 ,  C12N2535/10 ,  C12N2537/10 ,  C12N2539/10 ,  Y10T428/24273 ,  Y10T436/25 ,  Y10T436/25375

摘要： A chip useful for treating cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation. The chip comprises a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing the chip comprises applying a composition containing a crosslinkable temperature-responsive polymer on the surface of a baseboard to thereby form a coating film, crosslinking the coating film to thereby form the crosslinked product as described above and then forming a hole pattern on the coating film of the crosslinked product.

摘要翻译： 用于处理细胞等的芯片，其具有机构和结构，其中孔图案的尺寸任意改变，使得细胞可以容易地在散射或收集细胞中从孔中移出并离开，但几乎不能从 洗涤期间的孔或抗原刺激。 芯片包括作为构成部件的温度敏感性聚合物的交联体，并且在基板的表面上设置有具有孔图案的膜。 制造该芯片的方法包括在基板的表面上涂布含有交联性温度敏感性聚合物的组合物，从而形成涂膜，使涂膜交联从而形成如上所述的交联产物，然后在 交联产物的涂膜。

公开(公告)号：US20090181859A1

公开(公告)日：2009-07-16

申请号：US12337481

申请日：2008-12-17

申请人： Atsushi MURAGUCHI ,  Hiroyuki Kishi ,  Eiichi Tamiya ,  Masayasu Suzuki

发明人： Atsushi MURAGUCHI ,  Hiroyuki Kishi ,  Eiichi Tamiya ,  Masayasu Suzuki

IPC分类号： C40B30/06

CPC分类号： C07K16/082 ,  B01J2219/00317 ,  B01J2219/00743 ,  C07K2317/21 ,  C40B60/14 ,  G01N33/5302 ,  G01N33/54366 ,  G01N33/56972

摘要： A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.

摘要翻译： 微孔阵列芯片具有多个微孔，并用于在每个微孔中含有单个淋巴细胞标本，并以单个单位检测抗原特异性淋巴细胞; 其中所述微孔阵列芯片的形状和尺寸在每个微孔中仅包含一个淋巴细胞。 一种检测抗原特异性淋巴细胞的方法，包括以下步骤：向上述微孔阵列芯片中的每个微孔加入抗原，刺激淋巴细胞标本，以及检测与抗原反应的淋巴细胞标本。

公开(公告)号：US07362433B2

公开(公告)日：2008-04-22

申请号：US10550004

申请日：2003-03-24

申请人： Eiichi Tamiya ,  Toshiaki Tanaka ,  Toshiki Morita

发明人： Eiichi Tamiya ,  Toshiaki Tanaka ,  Toshiki Morita

IPC分类号： G01J3/42

CPC分类号： G01J3/10 ,  G01N21/253

摘要： The absorbance of samples in a number of wells on a microchamber array is efficiently read in a short period of time. The microchamber array is simultaneously irradiated with monochromatic light. Well-transmitted light is captured via a telecentric optical system (23) by an imaging camera (24) as an image, and the absorbance of the sample in each well is individually calculated.

摘要翻译： 微波炉阵列中多个孔中的样品的吸光度在短时间内有效读取。 微型阵列同时被单色光照射。 通过成像摄像机（24）作为图像通过远心光学系统（23）捕获透光的光，并且分别计算每个孔中的样品的吸光度。

公开(公告)号：US20060227320A1

公开(公告)日：2006-10-12

申请号：US10550004

申请日：2003-03-24

申请人： Eiichi Tamiya ,  Toshiaki Tanaka ,  Toshiki Morita

发明人： Eiichi Tamiya ,  Toshiaki Tanaka ,  Toshiki Morita

IPC分类号： G01J3/00

CPC分类号： G01J3/10 ,  G01N21/253

摘要： The absorbance of samples in a number of wells on a microchamber array is efficiently read in a short period of time. The microchamber array is simultaneously irradiated with monochromatic light. Well-transmitted light is captured via a telecentric optical system (23) by an imaging camera (24) as an image, and the absorbance of the sample in each well is individually calculated.

摘要翻译： 微波炉阵列中多个孔中的样品的吸光度在短时间内有效读取。 微型阵列同时被单色光照射。 通过成像摄像机（24）作为图像通过远心光学系统（23）捕获透光的光，并且分别计算每个孔中的样品的吸光度。

公开(公告)号：US20060091085A1

公开(公告)日：2006-05-04

申请号：US11256972

申请日：2005-10-25

申请人： Masaaki Kobayashi ,  Hiroshi Ichikawa ,  Eiichi Tamiya

发明人： Masaaki Kobayashi ,  Hiroshi Ichikawa ,  Eiichi Tamiya

IPC分类号： C02F1/38

CPC分类号： B01L3/50273 ,  B01L7/52 ,  B01L2300/0803 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0409 ,  G01N35/00029 ,  G01N2035/00158 ,  Y10T436/25375

摘要： A microchip used for a method for centrifugally dispensing a liquid sample by giving a revolving motion and a rotating motion to a rotary disk on which the microchip is mounted, includes a solution-pouring portion at a center thereof onto which the liquid sample is poured and channel patterns extending outward, with the solution-pouring portion as a center, and forming therein flow paths for the liquid sample and detection chambers which constitute portions for examining the liquid sample and in which the liquid sample is centrifugally dispensed. A centrifugal dispensing method using the microchip includes giving a revolving motion and a rotating motion to the rotary disk on which the microchip is mounted, thereby centrifugally dispensing the liquid sample in the detection chambers. A centrifugal dispenser using the microchip includes a rotary disk on which the microchip is mounted, means for revolving the rotary disk about a shaft and means for rotating the rotary disk about another shaft of the rotary disk.

摘要翻译： 用于通过向其上安装微芯片的旋转盘提供旋转运动和旋转运动来离心分配液体样品的方法的微芯片包括在其中注入液体样品的中心的溶液注入部分， 以溶液注入部为中心向外延伸的通道图案，并且在其中形成液体样品和检测室的流路，构成用于检查液体样品的部分，其中液体样品被离心分配。 使用微芯片的离心分配方法包括向安装有微芯片的旋转盘提供旋转运动和旋转运动，从而将液体样品离心分配到检测室中。 使用微芯片的离心分配器包括安装有微芯片的旋转盘，用于围绕轴旋转旋转盘的装置和用于使旋转盘围绕旋转盘的另一轴旋转的装置。

公开(公告)号：US20060051756A1

公开(公告)日：2006-03-09

申请号：US10512219

申请日：2003-04-22

申请人： Eiichi Tamiya ,  Masaaki Kobayyashi ,  Ichiro Nagashio

发明人： Eiichi Tamiya ,  Masaaki Kobayyashi ,  Ichiro Nagashio

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/502 ,  B01L3/50825 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/087 ,  B01L2400/0683

摘要： A gene detection tool comprising a sealable reaction chamber and a sealable detection chamber and a partition member positioned between the reaction chamber and the detection chamber that separates the reaction chamber and the detection chamber. The partition member is capable of unsealing; the reaction chamber is comprised of at least two members capable of forming a sealed reaction chamber by mutually fitting an opening means provided therein; and the detection chamber or reaction chamber comprises a gene detection element. A gene detection method in which a gene amplification operation and a gene detection operation are conducted in a sealed state using a gene detection device comprising a sealable reaction chamber and a sealable detection chamber, having a partition member which is positioned between the reaction chamber and the detection chamber and which separates the reaction chamber from the detection chamber, with test sample and gene amplification reaction solution being packed in said reaction chamber and a gene-binding agent capable of binding with a gene and generating a detectable signal being packed in said detection chamber. (1) At least the reaction chamber is imparted with conditions capable of amplifying the gene to be detected; (2) following the operation of (1), the partition member is unsealed while maintaining the gene detection tool in a sealed state; (3) through a passage created in the partition member by the unsealing, the solution in the reaction chamber is brought into contact with the gene-binding agent capable of binding the gene in the detection chamber and generating a detectable signal; and (4) the signal obtained is detected. A gene detection kit comprising the gene detection tool, a gene amplification reaction solution or gene amplification reagent, and a gene-binding agent capable of binding a gene and generating a detectable signal.

摘要翻译： 一种基因检测工具，其包括可密封的反应室和可密封的检测室以及位于所述反应室和所述检测室之间的分隔部件，所述分隔部件分离所述反应室和所述检测室。 分隔构件能够开封; 反应室由至少两个能够通过相互配合设置在其中的开启装置形成密封反应室的构件组成; 并且检测室或反应室包括基因检测元件。 一种基因检测方法，其中使用包括可密封反应室和可密封检测室的基因检测装置在密封状态下进行基因扩增操作和基因检测操作，所述基因检测装置具有位于反应室和反应室之间的分隔部件 检测室，并且将反应室与检测室分离，将测试样品和基因扩增反应溶液包装在所述反应室中，以及能够与基因结合并产生可检测信号的基因结合剂，所述基因结合剂包装在所述检测室 。 （1）至少使反应室具有能够扩增待检测基因的条件; （2）在（1）的操作之后，分隔构件在将基因检测工具保持在密封状态的同时被开封; （3）通过开封在分隔构件中产生的通道，使反应室中的溶液与能够结合检测室中的基因并产生可检测信号的基因结合剂接触; 和（4）检测所获得的信号。 一种基因检测试剂盒，其包含基因检测工具，基因扩增反应溶液或基因扩增试剂，以及能够结合基因并产生可检测信号的基因结合剂。