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15 条记录 (耗时 0.025 秒)

    Gene expression system based on codon translation efficiency
    12.
    发明授权
    Gene expression system based on codon translation efficiency 失效
    基于密码子翻译效率的基因表达系统

    公开(公告)号：US07901905B2

    公开(公告)日：2011-03-08

    申请号：US11077939

    申请日：2005-03-11

    申请人： Ian Hector Frazer

    发明人： Ian Hector Frazer

    IPC分类号： C12P21/02

    CPC分类号： C12N15/67

    摘要： The present invention discloses a method for modulating the production of a protein from a polynucleotide in a CHO cell by replacing at least one codon of the polynucleotide with a synonymous codon that has a higher or lower translation efficiency in the CHO cell than the codon it replaces, or by introducing into the CHO cell a polynucleotide that codes for an iso-tRNA which limits the rate of production of the polypeptide and which corresponds to a codon of the first polynucleotide. The present invention also discloses the use of a protein-encoding polynucleotide whose codon composition has been modified for enhanced production of the protein in CHO cells.

    摘要翻译： 本发明公开了一种通过用CHO细胞中具有更高或更低翻译效率的同义密码子代替多核苷酸的至少一个密码子来代替CHO细胞中的多核苷酸产生蛋白质的方法 或通过向CHO细胞中引入编码限制多肽的产生速率并且对应于第一多核苷酸的密码子的等位基因的RNA的多核苷酸。 本发明还公开了其密码子组成已被修饰以增加CHO细胞中蛋白质产生的蛋白质编码多核苷酸的用途。

    Compositions for eliciting an immune response
    13.
    发明授权
    Compositions for eliciting an immune response 有权
    用于引发免疫反应的组合物

    公开(公告)号：US08858951B2

    公开(公告)日：2014-10-14

    申请号：US10475203

    申请日：2002-04-18

    申请人： Ian Hector Frazer

    发明人： Ian Hector Frazer

    IPC分类号： A61K39/00 ,  C07K16/00

    CPC分类号： A61K39/12 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/5154 ,  A61K2039/5156 ,  A61K2039/5158 ,  A61K2039/53 ,  A61K2039/57 ,  C07K14/005 ,  C07K19/00 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20034 ,  Y02A50/467

    摘要： The invention is directed to the use of (i) a first antigen corresponding to a target antigen of interest, together with (ii) a second antigen, corresponding to a modified form of the target antigen, whose rate of intracellular proteolytic degradation is increased, enhanced or otherwise elevated relative to the first antigen, in compositions and methods for inducing both humoral and cellular immunity in an individual. The ability to provide compositions, which are capable of inducing both host-protective antibody and cell-mediated immune responses, facilitates the generation of immunogenic compositions capable of combating, inter alia, conditions that have long latency periods and, therefore, benefit from the dual approach of prophylaxis and therapy in one delivery.

    摘要翻译： 本发明涉及（i）对应于感兴趣的靶抗原的第一抗原与（ii）对应于细胞内蛋白水解降解速率增加的靶抗原修饰形式的第二抗原的用途， 在个体中诱导体液和细胞免疫的组合物和方法中相对于第一抗原增强或以其它方式升高。 提供能够诱导宿主保护性抗体和细胞介导的免疫应答的组合物的能力有助于产生免疫原性组合物，特别是能够抵抗具有长潜伏期并因此受益于双重作用的病症 一次性预防和治疗的方法。

    Nucleic acid sequence and methods for selectively expressing a protein in a target cell or tissue
    15.
    发明授权
    Nucleic acid sequence and methods for selectively expressing a protein in a target cell or tissue 有权
    核酸序列和用于在靶细胞或组织中选择性表达蛋白质的方法

    公开(公告)号：US06489141B1

    公开(公告)日：2002-12-03

    申请号：US09479645

    申请日：2000-01-07

    申请人： Ian Hector Frazer ,  Jian Zhou

    发明人： Ian Hector Frazer ,  Jian Zhou

    IPC分类号： C12N1564

    CPC分类号： C12N7/00 ,  A61K2039/5258 ,  C07K14/005 ,  C07K14/43595 ,  C12N15/67 ,  C12N15/85 ,  C12N2710/20022 ,  C12N2710/20023 ,  C12N2710/20051 ,  C12N2830/00 ,  C12N2830/60 ,  C12Q1/6897

    摘要： A synthetic polynucleotide and a method are disclosed for selectively expressing a protein in a target cell or tissue of a mammal. Selective expression is effected by replacing at least one existing codon of a parent polynucleotide encoding a protein of interest with a synonymous codon to produce a synthetic polynucleotide having altered translational kinetics compared to the parent polynucleotide. The synonymous codon is selected such that it has a higher translational efficiency in the target cell or tissue relative to one or more other cells or tissues of the mammal.

    摘要翻译： 公开了用于在哺乳动物的靶细胞或组织中选择性表达蛋白质的合成多核苷酸和方法。 通过使用同义密码子替换编码目标蛋白质的母体多核苷酸的至少一个现有密码子以产生与亲本多核苷酸相比具有改变的翻译动力学的合成多核苷酸来进行选择性表达。 选择同义密码子使得其相对于哺乳动物的一个或多个其它细胞或组织在靶细胞或组织中具有较高的翻译效率。