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    Method of making a genetically modified mammalian cell
    3.
    发明授权
    Method of making a genetically modified mammalian cell 失效
    制备遗传修饰的哺乳动物细胞的方法

    公开(公告)号：US07741079B2

    公开(公告)日：2010-06-22

    申请号：US11591969

    申请日：2006-11-02

    申请人： Ian Hector Frazer ,  Jian Zhou ,  Xiao Yi Sun, legal representative

    发明人： Ian Hector Frazer ,  Jian Zhou

    IPC分类号： C12P21/04 ,  C12N15/00 ,  C12N5/00

    CPC分类号： C12N7/00 ,  A61K2039/5258 ,  C07K14/005 ,  C07K14/43595 ,  C12N15/67 ,  C12N15/85 ,  C12N2710/20022 ,  C12N2710/20023 ,  C12N2710/20051 ,  C12N2830/00 ,  C12N2830/60 ,  C12Q1/6897

    摘要： A method of making a genetically modified mammalian cell, the method including selecting a first codon of a parent polynucleotide that encodes a polypeptide for replacement with a synonymous codon, wherein the synonymous codon is selected on the basis that it exhibits a higher translational efficiency in a first type of mammalian cell than the first codon in a comparison of translational efficiencies of codons in cells of the first type, replacing the first codon with the synonymous codon to form a synthetic polynucleotide, and introducing the synthetic polynucleotide into a mammalian cell to produce the genetically modified mammalian cell.

    摘要翻译： 一种制备遗传修饰的哺乳动物细胞的方法，所述方法包括选择编码用于替代同义密码子的多肽的亲本多核苷酸的第一密码子，其中所述同义密码子是基于其在 第一类型的哺乳动物细胞比第一密码子的细胞的密码子的翻译效率的比较，用同义密码子替换第一密码子以形成合成的多核苷酸，并将合成的多核苷酸引入到哺乳动物细胞中以产生 遗传修饰的哺乳动物细胞。

    Method and polynucleotides for determining translational efficiency of a codon
    4.
    发明授权
    Method and polynucleotides for determining translational efficiency of a codon 有权
    确定密码子翻译效率的方法和多核苷酸

    公开(公告)号：US06846671B2

    公开(公告)日：2005-01-25

    申请号：US09900345

    申请日：2001-07-06

    申请人： Ian Hector Frazer ,  Xiao Yi Sun

    发明人： Jian Zhou

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12Q1/02 ,  C12Q1/68 ,  C12N1/00 ,  C12N15/52 ,  C12N15/62 ,  C12N15/63

    CPC分类号： C12Q1/6897 ,  C12N15/67

    摘要： A method is disclosed for determining the translational efficiency of an individual codon in a cell. The method comprises introducing into the cell a synthetic construct comprising a reporter polynucleotide fused in frame with a tandem repeat of said individual codon, wherein said reporter polynucleotide encodes a reporter protein, and wherein said synthetic construct is operably linked to a regulatory polynucleotide and measuring expression of said reporter protein in said cell to determine the translational efficiency of said codon.

    摘要翻译： 公开了一种用于确定细胞中个体密码子的翻译效率的方法。 该方法包括向细胞中引入包含与所述单个密码子的串联重复序列融合的报告多核苷酸的合成构建体，其中所述报告多核苷酸编码报道蛋白，并且其中所述合成构建体可操作地连接于调控多核苷酸并测量表达 的所述细胞中的所述报告蛋白以确定所述密码子的翻译效率。

    METHOD FOR OPTIMISING GENE EXPRESSION USING SYNONYMOUS CODON OPTIMISATION
    5.
    发明申请
    METHOD FOR OPTIMISING GENE EXPRESSION USING SYNONYMOUS CODON OPTIMISATION 审中-公开
    使用SYNONYMOUS CODON优化优化基因表达的方法

    公开(公告)号：US20130074218A1

    公开(公告)日：2013-03-21

    申请号：US13619345

    申请日：2012-09-14

    申请人： Ian Hector FRAZER

    发明人： Ian Hector FRAZER

    IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N15/85 ,  C12N1/19 ,  C12N1/15 ,  C12N5/10 ,  C12N15/80 ,  C12N15/81 ,  A61K31/7088 ,  A61P35/00 ,  A61P9/00 ,  C12N15/11 ,  G01N33/53 ,  C12Q1/54 ,  A01K67/027

    CPC分类号： C12N15/67 ,  C12N15/8277

    摘要： The present invention discloses a method for modulating the quality of a selected phenotype that is displayed by an organism or part thereof and that results from the expression of a polypeptide-encoding polynucleotide by replacing at least one codon of that polynucleotide with a synonymous codon that has a higher or lower preference of usage by the organism or part thereof to produce the selected phenotype than the codon it replaces. The present invention is also directed to the use of a codon-modified polynucleotide so constructed for modulating the quality of a selected phenotype displayed by an organism or part thereof.

    摘要翻译： 本发明公开了一种用于调节由生物体或其部分显示的选择表型的质量的方法，以及由多肽编码多核苷酸的表达所产生的方法，该多核苷酸通过用同位密码替换该多核苷酸的至少一个密码子 生物体或其一部分使用的更高或更低的偏好以产生所选择的表型，而不是它所代替的密码子。 本发明还涉及如此构建的密码子修饰的多核苷酸的用途，以调节由生物体或其部分显示的选择的表型的质量。

    IMMUNOMODULATING COMPOSITIONS AND USES THEREFOR
    6.
    发明申请
    IMMUNOMODULATING COMPOSITIONS AND USES THEREFOR 审中-公开
    免疫组化及其用途

    公开(公告)号：US20090136546A1

    公开(公告)日：2009-05-28

    申请号：US12254752

    申请日：2008-10-20

    申请人： Ian Hector Frazer

    发明人： Ian Hector Frazer

    IPC分类号： A61K39/02 ,  A61K39/00

    CPC分类号： A61K39/39 ,  A61K2039/545 ,  A61K2039/55516 ,  A61K2039/55527

    摘要： The present invention discloses the use of an inhibitor of IL-10 function and an immune stimulator that stimulates the priming of an immune response to a target antigen, in methods and compositions for stimulating and prolonging host immune responses to the target antigen. The methods and compositions of the present invention are particularly useful in the treatment or prophylaxis of a range of conditions including pathogenic infections and cancers.

    摘要翻译： 本发明公开了用于刺激和延长对靶抗原的宿主免疫应答的方法和组合物中IL-10功能抑制剂和免疫刺激剂的用途，所述免疫刺激剂刺激对靶抗原的免疫应答的引发。 本发明的方法和组合物特别可用于治疗或预防包括病原体感染和癌症在内的一系列病症。

    Method and polynucleotides for determining translational efficiency of a codon
    9.
    发明授权
    Method and polynucleotides for determining translational efficiency of a codon 有权
    确定密码子翻译效率的方法和多核苷酸

    公开(公告)号：US07704742B2

    公开(公告)日：2010-04-27

    申请号：US11041806

    申请日：2005-01-24

    申请人： Ian Hector Frazer ,  Jian Zhou ,  Xiao Yi Sun, legal representative

    发明人： Ian Hector Frazer ,  Jian Zhou

    IPC分类号： C12N15/00 ,  C12N5/00 ,  C12Q1/00 ,  C12N15/63

    CPC分类号： C12Q1/6897 ,  C12N15/67

    摘要： A method is disclosed for determining the translational efficiency of an individual codon in a cell. The method includes introducing into the cell a synthetic construct including a reporter polynucleotide fused in frame with a tandem repeat of the individual codon, wherein the reporter polynucleotide encodes a reporter protein, and wherein the synthetic construct is operably linked to a regulatory polynucleotide; and measuring expression of the reporter protein in the cell to determine the translational efficiency of the codon.

    摘要翻译： 公开了一种用于确定细胞中个体密码子的翻译效率的方法。 该方法包括向细胞中引入包含与单个密码子的串联重复序列融合的报告多核苷酸的合成构建体，其中所述报道多核苷酸编码报道蛋白，并且其中所述合成构建体可操作地连接于调控多核苷酸; 并测量细胞中报道蛋白的表达以确定密码子的翻译效率。