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    Methods and primers for detecting target nucleic acid sequences
    11.
    发明授权
    Methods and primers for detecting target nucleic acid sequences 有权
    用于检测靶核酸序列的方法和引物

    公开(公告)号：US09273350B2

    公开(公告)日：2016-03-01

    申请号：US10862580

    申请日：2004-06-08

    申请人： David Mark Whitcombe ,  Jane Theaker ,  Neil James Gibson ,  Stephen Little

    发明人： David Mark Whitcombe ,  Jane Theaker ,  Neil James Gibson ,  Stephen Little

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6853 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2531/113 ,  C12Q2525/186 ,  C12Q2525/161 ,  C12Q2565/1025 ,  C12Q2561/119 ,  C12Q2525/301

    摘要： A method for the detection of a target nucleic acid, which method comprises contacting template nucleic acid from a sample with (i) a signalling system and (ii) a tailed nucleic acid primer having a template binding region and the tail comprising a linker and a target binding region, in the presence of appropriate nucleoside triphosphates and an agent for polymerisation thereof, under conditions such that the template binding region of the primer will hybridise to a complementary sequence in the template nucleic acid and be extended to form a primer extension product, separating any such product from the template whereupon the target binding region in the tail of the primer will hybridise to a sequence in the primer extension product corresponding to the target nucleic acid, and wherein any such target specific hybridisation causes a detectable change in the signalling system, such that the presence or absence of the target nucleic acid in the sample is detected by reference to the presence or absence of a detectable change in the signalling system.

    摘要翻译： 一种用于检测靶核酸的方法，所述方法包括使来自样品的模板核酸与（i）信号系统和（ii）具有模板结合区的尾部核酸引物接触，并且所述尾部包含接头和 在适当的核苷三磷酸和其聚合剂的存在下，在引物的模板结合区域将与模板核酸中的互补序列杂交并延伸以形成引物延伸产物的条件下， 将任何这样的产物与模板分离，由此引物尾部中的靶结合区域将与对应于靶核酸的引物延伸产物中的序列杂交，并且其中任何这样的靶特异性杂交引起信号系统中可检测的变化 ，使得通过参考前文检测样品中靶核酸的存在或不存在 信号系统中存在或不存在可检测的变化。

    Apparatus and method for controlling the temperature of a cryogen
    12.
    发明授权
    Apparatus and method for controlling the temperature of a cryogen 有权
    用于控制冷冻剂温度的装置和方法

    公开(公告)号：US09016076B2

    公开(公告)日：2015-04-28

    申请号：US12675282

    申请日：2008-08-27

    申请人： Kevin John Giardiniere ,  John Lewis Green ,  Ranajit Ghosh ,  Daniel James Gibson ,  Zbigniew Zurecki

    发明人： Kevin John Giardiniere ,  John Lewis Green ,  Ranajit Ghosh ,  Daniel James Gibson ,  Zbigniew Zurecki

    IPC分类号： F25D3/10 ,  G05D23/19 ,  F25D29/00

    CPC分类号： G05D23/1919 ,  F25D29/001

    摘要： A fluid spray device system (1) that maintains a resultant fluid discharge, or a material onto which the resultant fluid is discharged, within a predetermined range of a set-point temperature by regulating the flow rate of a throttling gas using a proportional valve (22). The resultant fluid has throttling gas and cryogenic fluid components. Both the throttling gas and cryogenic fluid are preferably supplied from a single tank (11) and the cryogenic fluid supply is pressure-regulated and includes a triaxial delivery hose (33) having a return line with a back-pressure regulator (54).

    摘要翻译： 一种流体喷射装置系统（1），其通过使用比例阀调节节流气体的流量，将所得流体排放物或所得流体排放到的材料排放到设定点温度的预定范围内 22）。 所得流体具有节流气体和低温流体组分。 节流气体和低温流体优选地从单个罐（11）供应，并且低温流体供应被压力调节并且包括具有带背压调节器（54）的返回管线的三轴输送软管（33）。

    Radiotherapeutic apparatus
    18.
    发明申请
    Radiotherapeutic apparatus 有权

    公开(公告)号：US20100329422A1

    公开(公告)日：2010-12-30

    申请号：US12739206

    申请日：2007-10-24

    申请人： Kevin Brown ,  Ralph Streamer ,  Paul Boxall ,  Duncan Bourne ,  Christopher James Gibson

    发明人： Kevin Brown ,  Ralph Streamer ,  Paul Boxall ,  Duncan Bourne ,  Christopher James Gibson

    IPC分类号： A61N5/10

    CPC分类号： A61N5/103 ,  A61N5/1036 ,  A61N5/1042

    摘要： A radiotherapeutic apparatus comprises a source able to emit a beam of therapeutic radiation along a beam axis, a multi-leaf collimator arranged to collimate the beam to a desired shape, wherein the source is rotateable about a rotation axis that is substantially orthogonal and intersects with the beam axis thereby to describe an arc around that axis, and further comprises a control means able to control the dose/time rate of the source, the rotation speed of the source, and the multi-leaf collimator position. The control means is arranged to receive a treatment plan in which the arc is divided into a plurality of notional arc-segments, and specifying the total dose for the arc-segment and a Start and end MLC position. It then controls the source in accordance with that plan over an first arc-segment such that at least one of the rotation speed and dose rate are constant and the multi-leaf collimator changes shape, and a second arc segment such that at least one of the rotation speed and dose rate are constant at a level different to the constant level adopted during the first arc-segment. It achieves this by calculating the total time required for the arc segment for a plurality of factors including an MLC leaf movement from a prescribed position at the start of the arc-segment to a prescribed position at the end of the arc-segment, at a maximum leaf speed, rotation of the source from the start to the end of the arc-segment at a maximum source rotation speed, delivery of the dose at a maximum dose rate per time, selecting the factor dictating the longest time, and Controlling the apparatus so that the selected factor operates at its respective maximum and the remaining factors are operated at a reduced rate selected to match that longest time, wherein the total time required for the arc segment for at least one factor relating to a moving geometry item is the greater of (a); a time required to complete the segment at a continuous defined upper speed for the geometry item and (b) a time required to accelerate the geometry item until it is travelling at the defined upper speed. Generally, the time required to accelerate the geometry item to the defined upper speed will include a time to accelerate the geometry item to that speed, and a further time to accelerate the geometry item beyond that speed and subsequently decelerate it until travelling at that speed.