公开(公告)号：US20240385177A1

公开(公告)日：2024-11-21

申请号：US18692497

申请日：2022-09-15

Applicant: KATAOKA CORPORATION ,  Kyoto Prefectural Public University Corporation

Inventor： Ping DAI ,  Yukimasa TAKEDA ,  Yoshinori HARADA ,  Junichi MATSUMOTO

IPC: G01N33/50 ,  C12N5/00 ,  C12N5/077

Abstract: The present disclosure relates to a new screening method to search for compounds that have browning-promoting or browning-inhibiting effects on brown adipocytes and that target cell membrane receptors.

公开(公告)号：US20230140027A1

公开(公告)日：2023-05-04

申请号：US17911527

申请日：2021-03-12

Applicant: KATAOKA CORPORATION

Inventor： Tadao MORISHITA ,  Junichi MATSUMOTO

IPC: C12M1/42 ,  C12M1/22 ,  C12N13/00

Abstract: The processing apparatus 200 of the present disclosure includes: a laser irradiation unit 21 capable of applying a laser to the photothermal conversion layer 13 of the cell culture tool 100 including the cell culture base layer 11 and the photothermal conversion layer 13; and a control unit 22 for controlling the laser irradiation unit 21. The control unit 22 includes a setting section 221 and an irradiation control section 222. The setting section 221 sets an irradiation region to be irradiated with the laser in the cell culture tool 100. The irradiation control section 222 controls the laser irradiation unit 21 based on the irradiation region such that the laser irradiation unit 21 applies the laser to a corresponding region of the photothermal conversion layer 13.

公开(公告)号：US20230082555A1

公开(公告)日：2023-03-16

申请号：US17779639

申请日：2020-11-18

Applicant: Kataoka Corporation ,  Kyoto Prefectural Public University Corporation

Inventor： Ping Dai ,  Yukimasa Takeda ,  Yoshinori Harada ,  Junichi Matsumoto

IPC: C12N5/077

Abstract: The present invention is primarily directed to provide a new composition for a medium which can be used for differentiation induction from somatic cells to brown adipocytes.
The present invention can include, for example, a composition for a medium used in differentiation induction from somatic cells to brown adipocytes, wherein the composition comprises the following seven components: a thyroid hormone receptor agonist, a glucocorticoid receptor agonist, a phosphodiesterase inhibitor, insulin, an ascorbic acid derivative, albumin, and an antibiotic.
According to the present invention, direct differentiation induction from somatic cells to brown adipocytes can be effectively performed. In addition, according to the present invention, it is possible to effectively maintain brown adipocytes.

公开(公告)号：US20230059404A1

公开(公告)日：2023-02-23

申请号：US17779567

申请日：2020-11-18

Applicant: Kataoka Corporation ,  Kyoto Prefectural Public University Corporation

Inventor： Ping Dai ,  Yukimasa Takeda ,  Yoshinori Harada ,  Junichi Matsumoto

IPC: A61K35/35 ,  C12N5/077

Abstract: The present invention is primarily directed to provide a new process capable of performing direct conversion of or induction from a somatic cell to brown adipocytes with low molecular weight compounds, without performing artificial gene transfer.
The present invention includes, for example, a process for producing brown adipocytes by direct differentiation induction from somatic cells, comprising a step of culturing a somatic cell in a serum-free differentiation induction medium in the presence of a selective PPARγ agonist and a cAMP inducer.
According to the present invention, direct conversion of or induction from somatic cells to brown adipocytes can be effectively performed without gene transfer. The brown adipocytes obtained by the present invention are useful as regenerative medicine, models of human brown adipocytes and human beige cells, and the like.

公开(公告)号：US20210071128A1

公开(公告)日：2021-03-11

申请号：US16627154

申请日：2019-05-30

Applicant: KATAOKA CORPORATION

Inventor： Junichi MATSUMOTO ,  Shoichi HONDA

IPC: C12M1/00 ,  C12M1/36

Abstract: The present invention is intended to provide a cell treatment apparatus and a method for treating cells that can suppress a variation of a treatment time of treating cells using laser light. The cell treatment apparatus of the present invention includes: a cell treatment chamber in which cells in a cell culture vessel are treated; an observation unit that can observe the cells; a laser projection unit that can project a laser image onto the cells; a laser moving unit that can move the laser projection unit; and a control unit. The laser projection unit includes: a laser light source; and a laser image generation portion that generates the laser image to be projected onto the cells from laser light oscillated from the laser light source. The control unit controls generation of the laser image by the laser image generation portion. By moving the laser moving unit from a projection start position of the laser image at one end of the cell culture vessel to a projection end position of the laser image at the other end of the cell culture vessel, the laser projection unit projects the laser image from the projection start position of the laser image at one end of the cell culture vessel to the projection end position of the laser image at the other end of the cell culture vessel.

公开(公告)号：US20180354076A1

公开(公告)日：2018-12-13

申请号：US15739395

申请日：2016-04-05

Applicant: KATAOKA CORPORATION

Inventor： Masami SUZUKI ,  Norio Nishi ,  Junichi MATSUMOTO

IPC: B23K26/38 ,  B23K26/00 ,  C12N5/074

CPC classification number: B23K26/38 ,  B23K26/0006 ,  B23K26/12 ,  C12M1/33 ,  C12N1/00 ,  C12N5/00 ,  C12N5/0696

Abstract: In order to cut a plurality of clumps having an approximately uniform shapes and approximately uniform dimensions out of a cell aggregate which has proliferated and appropriately eliminate contamination with fragments of different shapes or dimensions, when cutting the clumps of approximately uniform shape out of the cell aggregate which has proliferated, cutting lines along which the clumps of a specific shape are cut out are set such that the area of a peripheral part of the cell aggregate which is not cut by the cutting line exceeds the surface area of one of the clumps, and the cell aggregate is cut by irradiating with laser light in such a way as to trace the cutting lines.

公开(公告)号：US12270020B2

公开(公告)日：2025-04-08

申请号：US17312620

申请日：2019-12-13

Applicant: National Institute of Advanced Industrial Science and Technology ,  KATAOKA CORPORATION

Inventor： Kimio Sumaru ,  Toshiyuki Takagi ,  Toshiyuki Kanamori ,  Kana Morishita ,  Junichi Matsumoto

IPC: C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  C12N5/071

Abstract: A cell culture instrument configured to detach cells at a desired position and a cell processing method using the cell culture instrument. The cell culture instrument includes: a substrate; and a photoreactive layer having a photosolubility and a photothermal convertibility, wherein the photoreactive layer is laminated on the substrate, and the photoreactive layer includes a polymer having a photosolubility and a photothermal convertibility.

公开(公告)号：US12018283B2

公开(公告)日：2024-06-25

申请号：US16962190

申请日：2019-01-24

Applicant: KATAOKA CORPORATION ,  Kyoto Prefectural Public University Corporation

Inventor： Ping Dai ,  Yukimasa Takeda ,  Yoshinori Harada ,  Junichi Matsumoto ,  Ayumi Kusaka

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N2501/999 ,  C12N2506/1307

Abstract: The present invention chiefly aims to provide a process for directly inducing insulin-producing cells from somatic cells without performing artificial gene transfer. The present invention can include a process for producing an insulin-producing cell by inducing differentiation directly from a somatic cell, the process comprising a step of culturing the somatic cell in the presence of a cAMP inducer, and six members selected from the group consisting of a GSK3 inhibitor, a TGF-β inhibitor, a BMP inhibitor, a p53 inhibitor, a PI3K inhibitor, a Notch inhibitor and a RAR agonist, or all members thereof. The insulin-producing cells obtained by the present invention are useful in regenerative medicine and the like.

公开(公告)号：US20230278139A1

公开(公告)日：2023-09-07

申请号：US18175987

申请日：2023-02-28

Applicant: KATAOKA CORPORATION

Inventor： Takeshi YAMAMURA ,  Katsutoshi NAGASAKI ,  Keisuke HAYASHI

IPC: B23K26/24 ,  B23K26/082 ,  B23K26/12

CPC classification number: B23K26/24 ,  B23K26/082 ,  B23K26/123

Abstract: A welding method includes: arranging end portions of a first member and a second member next to each other facing an end surface of the end portions towards a laser device; performing rotational irradiation to the end surface of the end portions of the first member and the second member with laser light at a predetermined rotating diameter, wherein a spot diameter of the laser light is set to be equal to or larger than the rotating diameter, and wherein an irradiation region of the laser light by the rotational irradiation is set to extend over the first member and the second member.

公开(公告)号：US20230242862A1

公开(公告)日：2023-08-03

申请号：US18011911

申请日：2021-05-21

Applicant: KATAOKA CORPORATION

Inventor： Junichi MATSUMOTO ,  Tadao MORISHITA ,  Chiharu TABEI

IPC: C12M1/34 ,  G06N20/00 ,  G06T7/00

CPC classification number: C12M41/46 ,  G06N20/00 ,  G06T7/00

Abstract: The cell treatment apparatus of the present disclosure is a cell treatment apparatus including: an observation unit; a laser emitter; and a controller. The controller includes at least one processor that is configured to detect, using image data that includes the cell captured by an observation unit and a learned model capable of detecting a target cell or a non-target cell, a target cell or a non-target cell in the image data; set a region where the target cell is present or a region where the non-target cell is not present as a laser irradiation region to be subjected to laser irradiation by the laser emitter, and apply a laser emitted from the laser emitter to the laser irradiation region in the cell culture tool to treat the target cell.