公开(公告)号：US20110033381A1

公开(公告)日：2011-02-10

申请号：US12854007

申请日：2010-08-10

申请人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Yu Ogawa ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

发明人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Yu Ogawa ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

IPC分类号： A61K51/08 ,  C07K14/00 ,  A61K49/00

CPC分类号： A61K51/08 ,  C07K14/435 ,  C07K14/57563 ,  Y02P20/55

摘要： A precursor of molecular probe for imaging of pancreatic islets is provided. A polypeptide represented by any one of the following formulae (1) to (4), or a polypeptide having a homology with the foregoing polypeptide. *-DLSK* QMEEEAVRLFIEWLK* NGGPSSGAPPPSK-NH2(1)  *-LSK* QMEEEAVRLFIEWLK* NGGPSSGAPPPSK-NH2(2)   *-SK* QMEEEAVRLFIEWLK* NGGPSSGAPPPSK-NH2(3)    *-K* QMEEEAVRLFIEWLK* NGGPSSGAPPPSK-NH2,(4) wherein *- indicates that an α-amino group at an N-terminus is protected by a protecting group or modified with a modifying group having no electric charge; K* indicates that an amino group of a side chain of a lysine is protected by a protecting group; and —NH2 indicates that a carboxyl group at a C-terminus is amidated.

摘要翻译： 提供了用于胰岛成像的分子探针的前体。 由下式（1）至（4）中任一项表示的多肽或与前述多肽具有同源性的多肽。 * -DLSK * QMEEEAVRLFIEWLK * NGGPSSGAPPPSK-NH2（1）* -LSK * QMEEEAVRLFIEWLK * NGGPSSGAPPPSK-NH2（2）* -SK * QMEEEAVRLFIEWLK * NGGPSSGAPPPSK-NH2（3）* -K * QMEEEAVRLFIEWLK * NGGPSSGAPPPSK-NH2，（4）其中 *表示N-末端的α-氨基被保护基保护或用不带电的修饰基团修饰; K *表示赖氨酸的侧链的氨基被保护基保护; 并且-NH 2表示C末端的羧基被酰胺化。

公开(公告)号：US20070053790A1

公开(公告)日：2007-03-08

申请号：US10553336

申请日：2004-04-15

申请人： Kenji Nagakawa ,  Masaaki Teramoto ,  Yoshiyuki Kawase ,  Mitsuhiro Hoshijima

发明人： Kenji Nagakawa ,  Masaaki Teramoto ,  Yoshiyuki Kawase ,  Mitsuhiro Hoshijima

IPC分类号： G01N21/00

CPC分类号： B01L3/502707 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0825 ,  B01L2300/0838 ,  B01L2300/0887 ,  B01L2400/0406

摘要： The present invention relates to an analyzing tool (X1) including a reaction space (4) for allowing a particular component contained in a sample and a reagent to react with each other, and a reagent portion (51, 52) which is arranged in the reaction space (4) and which dissolves when the sample is supplied to the reaction space (4). The reagent portion (51, 52) includes a first part (51) and a second part (52) facing each other at a defining surface defining the reaction space (4).

摘要翻译： 本发明涉及一种分析工具（X 1），其包括用于使包含在样品中的特定成分和试剂彼此反应的反应空间（4）和布置在所述试剂部分中的试剂部分（51,52） 反应空间（4），并且当将样品供应到反应空间（4）时溶解。 试剂部分（51,52）包括在限定反应空间（4）的限定表面处彼此面对的第一部分（51）和第二部分（52）。

公开(公告)号：US20050014213A1

公开(公告)日：2005-01-20

申请号：US10496453

申请日：2003-01-06

申请人： Kenji Nagakawa ,  Tomomichi Tsujimoto ,  Susumu Nishino ,  Masaaki Teramoto ,  Yoshiyuki Kawase

发明人： Kenji Nagakawa ,  Tomomichi Tsujimoto ,  Susumu Nishino ,  Masaaki Teramoto ,  Yoshiyuki Kawase

IPC分类号： G01N21/78 ,  G01N33/52 ,  G01N33/66 ,  G01N33/92 ,  C12Q1/26

CPC分类号： G01N33/523 ,  G01N21/78 ,  G01N33/52 ,  G01N33/66 ,  G01N33/92

摘要： The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. An aqueous solution including a bipyridyl copper complex and glucose dehydrogenase is prepared. A filter paper is impregnated with the aqueous solution and dried. When a sample such as blood is applied to the filter paper, the bipyridyl copper complex produces reddish brown color in accordance with the glucose concentration, and the color produced in the complex is measured. This reaction is a single reaction and thus occurs in a short time (e.g., 5 seconds or less). Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

摘要翻译： 本发明提供一种可以在短时间内进行简单可靠的分析的比色法。 制备包含联吡啶铜络合物和葡萄糖脱氢酶的水溶液。 用水溶液浸渍滤纸并干燥。 当将诸如血液的样品施加到滤纸上时，联吡啶铜络合物根据葡萄糖浓度产生红棕色，并测量复合物中产生的颜色。 该反应是单一反应，因此在短时间（例如5秒或更短）内发生。 由于该反应既不需要过氧化氢也不需要氧气，因此测量值非常可靠。

公开(公告)号：US09238083B2

公开(公告)日：2016-01-19

申请号：US12893896

申请日：2010-09-29

申请人： Hideo Saji ,  Nobuya Inagaki ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

发明人： Hideo Saji ,  Nobuya Inagaki ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

IPC分类号： A61K51/08 ,  C07K14/00

CPC分类号： A61K51/08

摘要： A molecular probe for imaging of pancreatic islets is provided. The molecular probe includes a polypeptide represented by the following formula (1), or a polypeptide that has a homology with the foregoing polypeptide. (SEQ ID NO. 1) Z-HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPSX-NH2 (1) Wherein “X” represents a lysine residue, an amino group of a side chain of the lysine residue being labeled with a group represented by the following chemical formula (I), wherein A represents an aromatic hydrocarbon group or an aromatic heterocyclic group; R1 represents a substituent that contains 11C, 13N, 15O, 18F, 64Cu, 67Ga, 68Ga, 75Br, 76Br, 77Br, 99mTc, 111In, 123I, 124I, 125I, or 131I; R2 represents either a hydrogen atom, or a substituent different from that represented by R1; and R3 represents any one of a bond, an alkylene group having 1 to 6 carbon atoms, and an oxyalkylene group having 1 to 6 carbon atoms.

摘要翻译： 提供了用于胰岛成像的分子探针。 分子探针包括由下式（1）表示的多肽，或与前述多肽具有同源性的多肽。 （SEQ ID NO.1）Z-HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPSX-NH2（1）其中，“X”表示赖氨酸残基，赖氨酸残基的侧链的氨基用下述化学式（I）表示的基团标记， 其中A表示芳族烃基或芳族杂环基; R1表示含有11C，13N，150C，18F，64Cu，67Ga，68Ga，75Br，76Br，77Br，99mTc，111In，123I，124I，125I或131I的取代基; R2表示氢原子或与R1表示的取代基不同的取代基; R3表示碳原子数1〜6的亚烷基，碳原子数1〜6的氧亚烷基。

公开(公告)号：US08980220B2

公开(公告)日：2015-03-17

申请号：US12964486

申请日：2010-12-09

申请人： Hideo Saji ,  Nobuya Inagaki ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

发明人： Hideo Saji ,  Nobuya Inagaki ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

IPC分类号： C07K14/605 ,  G01N33/74 ,  A61K51/08

CPC分类号： G01N33/74 ,  A61K51/088 ,  C07K14/605

摘要： A molecular probe for use in imaging of pancreatic islets is provided. The molecular probe comprises a polypeptide represented by the following formula (1), (2), or (3), or a polypeptide having homology with the foregoing polypeptide, (SEQ ID NO. 1) Z-HGEGTFTSDLSXQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (1) (SEQ ID NO. 2) Z-HGEGTFTSDLSKQMEEEAVRLFIEWLXNGGPSSGAPPPS-NH2 (2) (SEQ ID NO. 3) B-HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (3) where, in the formulae (1) and (2), “X” represents a lysine residue, an amino group of a side chain of the lysine residue being labeled with a radioactive nuclide, and “Z—” indicates that an α-amino group at an N-terminus is not modified, or is modified with a modifying group having no electric charge; in the formula (3), “B—” indicates that an α-amino group at an N-terminus is labeled with a radioactive nuclide; and in the formulae (1), (2), and (3), “—NH2” indicates that a carboxyl group at a C-terminus is amidated.

摘要翻译： 提供了一种用于胰岛成像的分子探针。 分子探针包含由下式（1），（2）或（3）表示的多肽，或与前述多肽具有同源性的多肽（SEQ ID NO.1）Z-HGEGTFTSDLSXQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（1）（ SEQ ID NO.2）Z-HGEGTFTSDLSKQMEEEAVRLFIEWLXNGGPSSGAPPPS-NH2（2）（SEQ ID NO.3）B-HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（3）其中，在式（1）和（2）中，“X”表示赖氨酸残基， 赖氨酸残基的侧链的氨基被放射性核素标记，“Z-”表示N末端的α-氨基未被修饰，或者是用不具有电荷的修饰基团修饰 ; 在式（3）中，“B-”表示N末端的α-氨基用放射性核素标记; 在式（1），（2）和（3）中，“-NH 2”表示C末端的羧基被酰胺化。

公开(公告)号：USRE44522E1

公开(公告)日：2013-10-08

申请号：US12822869

申请日：2010-06-24

申请人： Kenji Nagakawa ,  Hideaki Yamaoka

发明人： Kenji Nagakawa ,  Hideaki Yamaoka

IPC分类号： G01N27/416

摘要： The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.

公开(公告)号：US20110206605A1

公开(公告)日：2011-08-25

申请号：US12964486

申请日：2010-12-09

申请人： Hideo Saji ,  Nobuya Inagaki ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

发明人： Hideo Saji ,  Nobuya Inagaki ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

IPC分类号： A61K51/08 ,  C07K14/00 ,  C07K1/13 ,  C12Q1/02

CPC分类号： G01N33/74 ,  A61K51/088 ,  C07K14/605

摘要： A molecular probe for use in imaging of pancreatic islets is provided. The molecular probe comprises a polypeptide represented by the following formula (1), (2), or (3), or a polypeptide having homology with the foregoing polypeptide, (SEQ ID NO. 1) Z-HGEGTFTSDLSXQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (1) (SEQ ID NO. 2) Z-HGEGTFTSDLSKQMEEEAVRLFIEWLXNGGPSSGAPPPS-NH2 (2) (SEQ ID NO. 3) B-HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (3) where, in the formulae (1) and (2), “X” represents a lysine residue, an amino group of a side chain of the lysine residue being labeled with a radioactive nuclide, and “Z—” indicates that an α-amino group at an N-terminus is not modified, or is modified with a modifying group having no electric charge; in the formula (3), “B—” indicates that an α-amino group at an N-terminus is labeled with a radioactive nuclide; and in the formulae (1), (2), and (3), “—NH2” indicates that a carboxyl group at a C-terminus is amidated.

摘要翻译： 提供了一种用于胰岛成像的分子探针。 分子探针包含由下式（1），（2）或（3）表示的多肽，或与前述多肽具有同源性的多肽（SEQ ID NO.1）Z-HGEGTFTSDLSXQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（1）（ SEQ ID NO.2）Z-HGEGTFTSDLSKQMEEEAVRLFIEWLXNGGPSSGAPPPS-NH2（2）（SEQ ID NO.3）B-HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（3）其中，在式（1）和（2）中，“X”表示赖氨酸残基， 赖氨酸残基的侧链的氨基被放射性核素标记，“Z-”表示N末端的α-氨基未被修饰，或者是用不具有电荷的修饰基团修饰 ; 在式（3）中，“B-”表示N末端的α-氨基用放射性核素标记; 在式（1），（2）和（3）中，“-NH 2”表示C末端的羧基被酰胺化。

公开(公告)号：US20110059483A1

公开(公告)日：2011-03-10

申请号：US12872837

申请日：2010-08-31

申请人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Yu Ogawa ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

发明人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Yu Ogawa ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

IPC分类号： C12Q1/04 ,  C07K14/00 ,  C12Q1/06

CPC分类号： A61K51/088

摘要： To provide a molecular probe for imaging of pancreatic islets. A molecular probe for use in imaging of pancreatic islets is provided. The molecular probe includes any one of the following polypeptides; polypeptides represented by the following formulae (1), (5), and (9); and polypeptides having homology with the foregoing polypeptides; Z-DLSXQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (1) Z-DLSKQMEEEAVRLFIEWLXNGGPSSGAPPPS-NH2 (5) B-DLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (9) where X in the formulae (1) and (5) and B—in the formula (9) indicate that an amino group is labeled with a group represented by the formula (I) below having an aromatic ring, wherein A represents either an aromatic hydrocarbon group or an aromatic heterocyclic group, R1 represents a substituent that contains radioactive iodine, R2 represents either a hydrogen atom or a substituent different from that represented by R1, and R3 represents any one of a bond, a methylene group, and an oxymethylene group.

摘要翻译： 为胰岛成像提供分子探针。 提供了一种用于胰岛成像的分子探针。 分子探针包括以下多肽之一; 由下式（1），（5）和（9）表示的多肽; 和与上述多肽具有同源性的多肽; Z-DLSXQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（1）Z-DLSKQMEEEAVRLFIEWLXNGGPSSGAPPPS-NH2（5）B-DLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（9）其中式（1）和（5）中的X和式（9）中的B-表示氨基 被具有芳环的式（I）表示的基团标记，其中A表示芳香族烃基或芳香族杂环基，R 1表示含有放射性碘的取代基，R 2表示氢原子或取代基 与R1表示的不同，R3表示键，亚甲基和甲醛基中的任一个。

公开(公告)号：US20090233322A1

公开(公告)日：2009-09-17

申请号：US12469403

申请日：2009-05-20

申请人： Kenji NAGAKAWA ,  Tomomichi TSUJIMOTO ,  Susumu NISHINO ,  Masaaki TERAMOTO ,  Yoshiyuki KAWASE

发明人： Kenji NAGAKAWA ,  Tomomichi TSUJIMOTO ,  Susumu NISHINO ,  Masaaki TERAMOTO ,  Yoshiyuki KAWASE

IPC分类号： C12Q1/26 ,  C12N9/02

CPC分类号： G01N21/78 ,  C12Q1/26 ,  C12Q1/32

摘要： The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

摘要翻译： 本发明提供一种可以在短时间内进行简单可靠的分析的比色法。 该方法包括将电子从分析物转移到通过使用氧化还原酶通过介质还原产生颜色的着色剂; 并通过测量着色剂中产生的颜色来对分析物进行定性或定量分析。 该方法的酶反应是单阶段反应，并且通过介体发生颜色产生反应。 因此，可以在短时间内进行测量。 由于该反应既不需要过氧化氢也不需要氧气，因此测量值非常可靠。

公开(公告)号：US07550273B2

公开(公告)日：2009-06-23

申请号：US10476298

申请日：2003-01-06

申请人： Kenji Nagakawa ,  Tomomichi Tsujimoto ,  Susumu Nishino ,  Masaaki Teramoto ,  Yoshiyuki Kawase

发明人： Kenji Nagakawa ,  Tomomichi Tsujimoto ,  Susumu Nishino ,  Masaaki Teramoto ,  Yoshiyuki Kawase

IPC分类号： C12Q1/26

CPC分类号： G01N21/78 ,  C12Q1/26 ,  C12Q1/32

摘要： The present invention provides a calorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

摘要翻译： 本发明提供一种能够在短时间内进行简单可靠的分析的量热法。 该方法包括将电子从分析物转移到通过使用氧化还原酶通过介质还原产生颜色的着色剂; 并通过测量着色剂中产生的颜色来对分析物进行定性或定量分析。 该方法的酶反应是单阶段反应，并且通过介体发生颜色产生反应。 因此，可以在短时间内进行测量。 由于该反应既不需要过氧化氢也不需要氧气，因此测量值非常可靠。