利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

30 条记录 (耗时 0.025 秒)

    Starches via modification of expression of starch biosynthetic enzyme genes
    11.
    发明授权
    Starches via modification of expression of starch biosynthetic enzyme genes 失效
    通过修饰淀粉生物合成酶基因表达的淀粉

    公开(公告)号：US06376749B1

    公开(公告)日：2002-04-23

    申请号：US09257894

    申请日：1999-02-25

    申请人： Karen E. Broglie ,  Natalie L. Hubbard ,  Theodore M. Klein ,  Jonathan E. Lightner

    发明人： Karen E. Broglie ,  Natalie L. Hubbard ,  Theodore M. Klein ,  Jonathan E. Lightner

    IPC分类号： C12N1582

    CPC分类号： C12N9/107 ,  A23L29/212 ,  C12N15/8245

    摘要： The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch branching enzyme enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of corn comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch branching enzyme structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination, and (2) transforming corn with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed corn compared to the fine structure of starch derived from corn not possessing said chimeric gene.

    摘要翻译： 本发明公开了利用cDNA克隆构建用于抑制玉米淀粉分支酶酶活性的有义和反义基因。 更具体地说，本发明涉及一种控制来自玉米粒的淀粉的淀粉精细结构的方法，包括：（1）制备嵌合基因，其包含编码淀粉分支酶结构基因或其片段的核酸片段，其可操作地连接 在上游侧的任何正义或反义方向，编码引导玉米胚乳组织中的基因表达的启动子的核酸片段，并且在下游侧可操作地连接到编码转录终止的合适调控序列的核酸片段，和（ 2）用所述嵌合基因转化玉米，其中所述嵌合基因的表达导致衍生自所述转化玉米的淀粉的淀粉的精细结构的改变与源自不具有所述嵌合基因的玉米的淀粉的精细结构相反。

    Gene expression modulating element
    14.
    发明授权
    Gene expression modulating element 有权

    公开(公告)号：US07790874B2

    公开(公告)日：2010-09-07

    申请号：US12626962

    申请日：2009-11-30

    申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

    发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

    IPC分类号： C12N15/63 ,  C12N15/67 ,  C12N15/82 ,  A01H1/00

    CPC分类号： C12N15/8216

    摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

    Gene Expression Modulating Element
    15.
    发明申请
    Gene Expression Modulating Element 审中-公开

    公开(公告)号：US20100077514A1

    公开(公告)日：2010-03-25

    申请号：US12626960

    申请日：2009-11-30

    申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

    发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

    IPC分类号： A01H5/00 ,  C07H21/00 ,  C12N5/10

    CPC分类号： C12N15/8216

    摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

    Stable transformation of plant cells
    19.
    发明授权
    Stable transformation of plant cells 失效
    植物细胞稳定转化

    公开(公告)号：US5990387A

    公开(公告)日：1999-11-23

    申请号：US319352

    申请日：1994-10-06

    申请人： Dwight T. Tomes ,  Arthur Weissinger ,  John C. Sanford ,  Theodore M. Klein

    发明人： Dwight T. Tomes ,  Arthur Weissinger ,  John C. Sanford ,  Theodore M. Klein

    IPC分类号： C12N15/29 ,  C12N15/31 ,  C12N15/82 ,  C12N15/00 ,  A01H1/06 ,  A01H4/00

    CPC分类号： C12N15/8207

    摘要： The invention provides methods for producing a fertile, stably transformed, Zea mays plant. The methods comprise the steps of (a) providing a foreign DNA comprising an expression vector carrying a gene encoding an agronomic trait; (b) providing a maize embryogenic callus, suspension culture, or immature embryo isolated from a plant; (c) introducing the foreign DNA into the embryogenic callus, suspension culture or immature embryo isolated from a plant by one or more microparticle bombardments; and (d) regenerating fertile transgenic Zea mays plant.

    摘要翻译： 本发明提供了生产可育，稳定转化的玉米种植物的方法。 所述方法包括以下步骤：（a）提供包含携带编码农艺性状的基因的表达载体的外源DNA; （b）提供从植物分离的玉米胚发生愈伤组织，悬浮培养物或未成熟胚; （c）通过一个或多个微粒轰击将外源DNA引入到从植物分离的胚性愈伤组织，悬浮培养物或未成熟胚中; 和（d）再生可育转基因玉米植物。