公开(公告)号：US20130211186A1

公开(公告)日：2013-08-15

申请号：US13763908

申请日：2013-02-11

Applicant: WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION

Inventor： Jon Michael Oatley ,  Melissa Joan Oatley

IPC: C12N5/076 ,  C12N5/071 ,  C12M1/00 ,  C12N5/073

CPC classification number: C12N5/061 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2227/101 ,  A01K2227/108 ,  A01K2267/02 ,  A61B17/425 ,  A61D19/00 ,  A61K35/52 ,  C12M21/00 ,  C12N5/0602 ,  C12N5/0603 ,  C12N5/0656 ,  C12N5/0683 ,  C12N15/873 ,  C12N2500/05 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/46 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/21 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/246 ,  C12N2510/04 ,  C12N2533/90

Abstract: The present invention relates to the production and culture of undifferentiated spermatogonial stem cells that can be maintained long term and are feeder free. The resultant feeder-free populations can be used in any of a number of protocols including the generation of progeny bulls. The present invention includes novel methods required for the successful enrichment of bovine spermatogonial stem cells, novel cell lines and other components used for the same, as well as the resultant stem cell compositions.

公开(公告)号：US20040236089A1

公开(公告)日：2004-11-25

申请号：US10884115

申请日：2004-07-01

Applicant: Washington State University Research Foundation

Inventor： Rodney B. Croteau ,  Anne Schoendorf ,  Stefan Jennewein

IPC: C07H021/04

CPC classification number: C12N9/0077 ,  A01K2217/05 ,  C12P17/02

Abstract: Oxygenase enzymes and the use of such enzymes to produce paclitaxel (Taxolnull), related taxoids, as well as intermediates in the Taxol biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the oxygenase enzymes.

Abstract translation: 公开了加氧酶和这些酶在紫杉醇生物合成途径中产生紫杉醇（Taxol TM），相关紫杉类以及中间体的用途。 还公开了编码加氧酶的核酸序列。

公开(公告)号：US20040216854A1

公开(公告)日：2004-11-04

申请号：US10854106

申请日：2004-05-26

Applicant: Nile Fiber Pulp & Paper, Inc. ,  Washington State University Research Foundation

Inventor： Ernett Altheimer ,  Michael P. Wolcott

IPC: D21C003/00 ,  D21C003/02 ,  D21D001/02

CPC classification number: D21H11/12 ,  B27J1/00 ,  B27L11/02 ,  B27L11/08 ,  B27N3/04 ,  C08J5/045 ,  D21B1/00 ,  D21C5/00 ,  D21C9/163 ,  D21H27/18 ,  Y10T428/249925

Abstract: Composite panels and pulp, and paper products of the pulp, are produced from Arundo donax. In the fabrication of the composite panels, Arundo donax is comminuted to a suitable size, combined with a binder, and consolidated into panels that meet standards for construction and/or furniture grade panels. The Arundo donax particulates may be combined with wood particulates to produce a mixed furnish that can be used in the preparation of composite panels. Comminuted Arundo donax is treated, in conventional pulping processes, to produce a high tensile strength pulp that can be used in the production of paper. The pulp has a lighter color than wood pulp, and thereby uses less bleaching chemicals to achieve a desired whiteness. The pulp can be combined with wood pulp to produce a variety of products.

公开(公告)号：US20040010817A1

公开(公告)日：2004-01-15

申请号：US10410031

申请日：2003-04-09

Applicant: Washington State University Research Foundation

Inventor： Jay M. Shockey ,  Judy Schnurr ,  John A. Browse

IPC: A01H001/00 ,  C12N015/82 ,  C07H021/04 ,  C12N015/87 ,  C12N005/04

CPC classification number: C12N9/93 ,  A23D9/00 ,  C12N9/00 ,  C12N15/8247

Abstract: The present invention relates to genes encoding plant acyl-CoA synthetases and methods of their use. In particular, the present invention is related to plant acyl-coenzyme A synthetases. The present invention encompasses both native and recombinant wild-type forms of the enzymes, as well as mutant and variant forms, some of which possess altered characteristics relative to the wild-type enzyme. The present invention also relates to methods of using acyl-CoA synthetases, including altered expression in transgenic plants and expression in prokaryotes and cell culture systems.

Abstract translation: 本发明涉及编码植物酰基辅酶A合成酶的基因及其使用方法。 特别地，本发明涉及植物酰基辅酶A合成酶。 本发明包括天然和重组的野生型形式的酶，以及突变体和变体形式，其中一些相对于野生型酶具有改变的特征。 本发明还涉及使用酰基辅酶A合成酶的方法，包括转基因植物中改变的表达和在原核生物和细胞培养系统中的表达。

公开(公告)号：US20030097676A1

公开(公告)日：2003-05-22

申请号：US10119136

申请日：2002-04-09

Applicant: Washington State University Research Foundation

Inventor： Jay M. Shockey ,  Judy Schnurr ,  John A. Browse

IPC: A01H001/00 ,  C12N015/82 ,  C07H021/04 ,  C12N005/04

CPC classification number: C12N9/93 ,  A23D9/00 ,  C12N9/00 ,  C12N15/8247

Abstract: The present invention relates to genes encoding plant acyl-CoA synthetases and methods of their use. In particular, the present invention is related to plant acyl-coenzyme A synthetases. The present invention encompasses both native and recombinant wild-type forms of the enzymes, as well as mutant and variant forms, some of which possess altered characteristics relative to the wild-type enzyme. The present invention also relates to methods of using acyl-CoA synthetases, including altered expression in transgenic plants and expression in prokaryotes and cell culture systems.

Abstract translation: 本发明涉及编码植物酰基辅酶A合成酶的基因及其使用方法。 特别地，本发明涉及植物酰基辅酶A合成酶。 本发明包括天然和重组的野生型形式的酶，以及突变体和变体形式，其中一些相对于野生型酶具有改变的特征。 本发明还涉及使用酰基辅酶A合成酶的方法，包括转基因植物中改变的表达和在原核生物和细胞培养系统中的表达。

公开(公告)号：US20030037357A1

公开(公告)日：2003-02-20

申请号：US09906419

申请日：2001-07-16

Applicant: Washington State University Research Foundation

Inventor： Jay M. Shockey ,  Judy Schnurr ,  John A. Browse

IPC: A01H005/00 ,  C07H021/04 ,  C12N015/82

CPC classification number: C12N9/93 ,  A23D9/00 ,  C12N15/8247

Abstract: The present invention relates to genes encoding plant acyl-CoA synthetases and methods of their use. In particular, the present invention is related to plant acyl-coenzyme A synthetases. The present invention encompasses both native and recombinant wild-type forms of the enzymes, as well as mutant and variant forms, some of which possess altered characteristics relative to the wild-type enzyme. The present invention also relates to methods of using acyl-CoA synthetases, including altered expression in transgenic plants and expression in prokaryotes and cell culture systems.

公开(公告)号：US20010046694A1

公开(公告)日：2001-11-29

申请号：US09874562

申请日：2001-06-04

Applicant: Washington State University Research Foundation

Inventor： Rodney B. Croteau ,  Bernd M. Lange

IPC: C12N009/02 ,  C07H021/04 ,  C12N005/04 ,  C12P021/02

CPC classification number: C12N9/0006

Abstract: The present invention relates to isolated DNA sequences which code for the expression of plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein, such as the sequence presented in SEQ ID NO:1 which encodes a 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein from peppermint (Mentha x piperita). Additionally, the present invention relates to isolated plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase, to modified host cells transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence of the invention.

Abstract translation: 本发明涉及编码植物1-脱氧-D-木酮糖-5-磷酸还原异构酶蛋白质的表达的分离的DNA序列，例如编码1-脱氧-D-木酮糖的SEQ ID NO：1所示的序列 -5-薄荷薄荷（Mentha x piperita）的5-磷酸还原异构酶蛋白。 另外，本发明涉及分离的植物1-脱氧-D-木酮糖-5-磷酸还原异构酶蛋白。 在其它方面，本发明涉及可复制的重组克隆载体，其包含编码植物1-脱氧-D-木酮糖-5-磷酸还原异构酶的核酸序列转化，转染，感染和/或注射的修饰的宿主细胞 与本发明的重组克隆载体和/或DNA序列。

公开(公告)号：US09695450B2

公开(公告)日：2017-07-04

申请号：US14308456

申请日：2014-06-18

Applicant: Washington State University Research Foundation

Inventor： John A. Browse ,  James G. Wallis ,  Jennifer L. Watts

IPC: C12N15/82 ,  C12P7/64 ,  C12N9/02 ,  C12N15/80 ,  C12N15/85

CPC classification number: C12P7/6409 ,  C12N9/0071 ,  C12N9/0083 ,  C12N15/80 ,  C12N15/8241 ,  C12N15/8247 ,  C12N15/85

Abstract: The amino acid and nucleic acid sequences of a Δ5-desaturase enzyme and a Δ8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.

公开(公告)号：US09528115B1

公开(公告)日：2016-12-27

申请号：US14208543

申请日：2014-03-13

Applicant: The United States of America, as represented by the Secretary of Agriculture ,  Washington State University Research Foundation

Inventor： David M Weller ,  Linda S Thomashow ,  Dmitri V Mavrodi ,  Mingming Yang ,  Jibin Zhang

IPC: C12N15/78

CPC classification number: C12N15/52 ,  A01N43/36 ,  A01N63/02 ,  C12P17/10

Abstract: In exemplary embodiments, the invention provides Pseudomonas fluorescens 2-79 strains having pyrrolnitrin biosynthetic genes for biocontrol of soilborne diseases.

Abstract translation: 在示例性实施方案中，本发明提供了具有用于生物防治土壤传播疾病的吡咯烷生物合成基因的荧光假单胞菌2-79菌​​株。

公开(公告)号：US09290524B2

公开(公告)日：2016-03-22

申请号：US13837904

申请日：2013-03-15

Applicant: WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION

Inventor： Jinwen Zhang ,  Yu Fu

IPC: C07F7/10 ,  C07F7/18

CPC classification number: C07F7/1896 ,  C01B31/043 ,  C01B32/23

Abstract: Methods for producing functionalized graphene from graphite oxide or graphite are disclosed in which ionic liquids are used as a reaction medium to promote chemical functionalization of the graphene through electrochemically interacted exfoliation of graphene sheets.

Abstract translation: 公开了从石墨氧化物或石墨生产官能化石墨烯的方法，其中使用离子液体作为反应介质以通过石墨烯片的电化学相互作用的剥离来促进石墨烯的化学官能化。