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    Modified nucleotides
    11.
    发明申请
    Modified nucleotides 有权
    修饰的核苷酸

    公开(公告)号：US20100292452A1

    公开(公告)日：2010-11-18

    申请号：US12804352

    申请日：2010-07-20

    申请人： John Milton ,  Xiaolin Wu ,  Mark Smith ,  Joseph Brennan ,  Colin Barnes ,  Xiaohai Liu ,  Silke Ruediger

    发明人： John Milton ,  Xiaolin Wu ,  Mark Smith ,  Joseph Brennan ,  Colin Barnes ,  Xiaohai Liu ,  Silke Ruediger

    IPC分类号： C07H21/00 ,  C07H21/04

    CPC分类号： C12Q1/6869 ,  C07H1/00 ,  C07H19/06 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12Q2525/186 ,  C12Q2535/113 ,  Y02P20/55 ,  C12Q2525/197

    摘要： The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3′-OH blocking group covalently attached thereto, such that the 3′ carbon atom has attached a group of the structure —O—Z wherein Z is any of —C(R′)2-O—R″, —C(R′)2-N(R″)2, —C(R′)2-N(H)R″, —C(R′)2-S—R″ and —C(R′)2-F, wherein each R″ is or is part of a removable protecting group; each R′ is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R′)2 represents an alkylidene group of formula ═C(R′″)2 wherein each R′″ may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R″ is exchanged for H or, where Z is —C(R′)2-F, the F is exchanged for OH, SH or NH2, preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3′OH; with the proviso that where Z is —C(R′)2-S—R″, both R′ groups are not H.

    摘要翻译： 本发明提供了修饰的核苷酸或核苷分子，其包含嘌呤或嘧啶碱基和核糖或脱氧核糖糖部分，其具有共价连接到其上的可移除的3'-OH封闭基团，使得3'碳原子连接一组结构-O -Z其中Z是-C（R'）2 -O-R“，-C（R'）2 -N（R”）2，-C（R'）2 -N（H）R“ -C（R'）2 -S-R“和-C（R'）2 -F，其中每个R”为或可移除的保护基团的一部分; 每个R'独立地是氢原子，烷基，取代的烷基，芳基烷基，烯基，炔基，芳基，杂芳基，杂环，酰基，氰基，烷氧基，芳氧基，杂芳氧基或酰氨基，或通过连接基团连接的可检测标记; 或（R'）2表示式≡C（R'“）2的亚烷基，其中每个R”可以相同或不同，并且选自氢和卤素原子和烷基; 并且其中所述分子可以反应以产生其中每个R“被交换为H的中间体，或其中Z是-C（R'）2 -F，F被交换成OH，SH或NH 2，优选OH，其中 中间体在水性条件下解离得到具有游离3'OH的分子; 条件是其中Z是-C（R'）2 -S-R“，两个R'基团不是H.

    Modified nucleotides
    12.
    发明申请
    Modified nucleotides 有权
    修饰的核苷酸

    公开(公告)号：US20090325172A1

    公开(公告)日：2009-12-31

    申请号：US12455397

    申请日：2009-06-01

    申请人： John Milton ,  Xiaolin Wu ,  Mark Smith ,  Joseph Brennan ,  Colin Barnes ,  Xiaohai Liu ,  Silke Ruediger

    发明人： John Milton ,  Xiaolin Wu ,  Mark Smith ,  Joseph Brennan ,  Colin Barnes ,  Xiaohai Liu ,  Silke Ruediger

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C07H1/00 ,  C07H19/06 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12Q2525/186 ,  C12Q2535/113 ,  Y02P20/55 ,  C12Q2525/197

    摘要： The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3′-OH blocking group covalently attached thereto, such that the 3′ carbon atom has attached a group of the structure —O-Z wherein Z is any of —C(R′)2-O—R″, —C(R′)2-N(R″)2, —C(R′)2-N(H)R″, —C(R′)2-S—R″ and —C(R′)2-F, wherein each R″ is or is part of a removable protecting group; each R′ is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R′)2 represents an alkylidene group of formula ═C(R′″)2 wherein each R′″ may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R″ is exchanged for H or, where Z is —C(R′)2-F, the F is exchanged for OH, SH or NH2, preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3′OH; with the proviso that where Z is —C(R′)2-S—R″, both R′ groups are not H.

    摘要翻译： 本发明提供了修饰的核苷酸或核苷分子，其包含嘌呤或嘧啶碱基和核糖或脱氧核糖糖部分，其具有共价连接到其上的可移除的3'-OH封闭基团，使得3'碳原子连接有一组结构-OZ 其中Z是-C（R'）2 -O-R“，-C（R'）2 -N（R”）2，-C（R'）2 -N（H）R“ ，-C（R'）2 -S-R“和-C（R'）2 -F，其中每个R”或是可除去的保护基团的一部分; 每个R'独立地是氢原子，烷基，取代的烷基，芳基烷基，烯基，炔基，芳基，杂芳基，杂环，酰基，氰基，烷氧基，芳氧基，杂芳氧基或酰氨基，或通过连接基团连接的可检测标记; 或（R'）2表示式-C（R“'）2的亚烷基，其中每个R”可以相同或不同，并且选自氢和卤素原子和烷基; 并且其中所述分子可以反应以产生其中每个R“被交换为H的中间体，或者其中Z是-C（R'）2-F，F被交换成OH，SH或NH 2，优选OH， 该中间体在水性条件下解离以得到具有游离3'OH的分子; 条件是其中Z是-C（R'）2 -S-R“，两个R'基团都不是H.

    INFLUENZA VIRUS NUCLEIC ACID MICROARRAY AND METHOD OF USE
    14.
    发明申请
    INFLUENZA VIRUS NUCLEIC ACID MICROARRAY AND METHOD OF USE 审中-公开
    流感病毒核酸微生物及其使用方法

    公开(公告)号：US20090088331A1

    公开(公告)日：2009-04-02

    申请号：US11936530

    申请日：2007-11-07

    申请人： Xiaolin Wu ,  Cassio S. Baptista ,  David J. Munroe

    发明人： Xiaolin Wu ,  Cassio S. Baptista ,  David J. Munroe

    IPC分类号： C40B30/04 ,  C40B40/06

    CPC分类号： C12Q1/6837 ,  C12Q1/702

    摘要： The present invention relates generally to methods of detecting and identifying known and unknown viruses using hybridization microarrays to essentially all known influenza virus nucleotide sequences of at least one type that infect at least one species, the sequencing of nucleotides which hybridize to the microarrays and analysis of the hybridized sequences with existing databases, thus identifying existing or new subtypes of viruses. The present invention also relates to methods of use of the microarrays of the invention for the detection of influenza viruses, including variant influenza viruses. The method includes the use of a non-specific PCR amplification method to amplify sample nucleic acids.

    摘要翻译： 本发明一般涉及使用杂交微阵列检测和鉴定已知和未知病毒的方法，基本上所有已知的感染至少一种的至少一种类型的流感病毒核苷酸序列，与微阵列杂交的核苷酸的测序和 与现有数据库的杂交序列，从而识别现有的或新的亚型的病毒。 本发明还涉及本发明的微阵列用于检测流感病毒（包括变种流感病毒）的方法。 该方法包括使用非特异性PCR扩增方法来扩增样品核酸。

    Viral nucleic acid microarray and method of use
    15.
    发明申请
    Viral nucleic acid microarray and method of use 审中-公开
    病毒核酸微阵列及其使用方法

    公开(公告)号：US20080003565A1

    公开(公告)日：2008-01-03

    申请号：US11800080

    申请日：2007-05-02

    申请人： Cassio Baptista ,  Xiaolin Wu ,  David Munroe

    发明人： Cassio Baptista ,  Xiaolin Wu ,  David Munroe

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/70 ,  C12Q1/6837

    摘要： The present invention relates generally to methods of detecting and identifying known and unknown viruses using hybridization microarrays to known conserved and non-conserved viral nucleotide sequences, the sequencing of nucleotides which hybridize to the microarrays and analysis of the hybridized sequences with existing databases, thus identifying existing or new subtypes of viruses.

    摘要翻译： 本发明一般涉及使用已知的保守和非保守的病毒核苷酸序列的杂交微阵列检测和鉴定已知和未知病毒的方法，与微阵列杂交的核苷酸的测序以及与现有数据库的杂交序列的分析，从而鉴定 现有或新的病毒亚型。

    Image/information display system and method based on temporal psycho-visual modulation
    18.
    发明授权
    Image/information display system and method based on temporal psycho-visual modulation 有权
    基于时间心理视觉调制的图像/信息显示系统和方法

    公开(公告)号：US09578314B2

    公开(公告)日：2017-02-21

    申请号：US14128509

    申请日：2012-06-21

    申请人： Xiaolin Wu ,  Guangtao Zhai

    发明人： Xiaolin Wu ,  Guangtao Zhai

    IPC分类号： H04N7/12 ,  H04N13/04 ,  H04N13/00 ,  G06F3/14

    CPC分类号： H04N13/30 ,  G06F3/1423 ,  G09G2310/0297 ,  G09G2370/20 ,  H04N13/144 ,  H04N13/341 ,  H04N2013/40

    摘要： An image/information display system and method based on temporal psychovisual modulation in the technical field of image/video processing and electro-optical display. The system includes a high speed display device and an auxiliary viewing device cooperating with each other and interacting with a human visual system. The auxiliary viewing device realizes the temporal amplitude modulation of the composition basic sequence by a method of controlling the light intensity transfer rate from the display plane of the high speed display device to the human eye.

    摘要翻译： 一种基于图像/视频处理和电光显示技术领域的时间精神视觉调制的图像/信息显示系统和方法。 该系统包括彼此协作并与人类视觉系统相互作用的高速显示装置和辅助观察装置。 辅助观看装置通过控制从高速显示装置的显示面到人眼的光强度传递速率的方法来实现组成基本序列的时间幅度调制。