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公开(公告)号：US20050266465A1
公开(公告)日：2005-12-01
申请号：US11132722
申请日：2005-05-18
申请人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski , Stephens Adam
发明人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski , Stephens Adam
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
摘要翻译： 本发明提供了干扰素-α多肽和缀合物，以及编码多肽的核酸。 本发明还包括包含这些多肽，缀合物和核酸的组合物; 含有或表达多肽，缀合物和核酸的细胞; 制备多肽，缀合物和核酸的方法; 以及使用多肽，缀合物和核酸的方法。
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公开(公告)号：US5672691A
公开(公告)日：1997-09-30
申请号：US434718
申请日：1995-05-04
IPC分类号： C07K14/195 , C07K14/36 , C07K14/41 , C12N1/21 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/19 , G01N33/50 , G01N33/53 , C07K1/22
CPC分类号： C07K14/36
摘要： The present invention concerns a process for the isolation of recombinant core streptavidin in which host cells are transformed with a DNA coding for core streptavidin, the transformed host cells are cultured under suitable conditions, the DNA coding for core streptavidin is expressed and the recombinant core streptavidin is isolated from the host cells or the culture medium, wherein a DNA coding for core streptavidin is used which has (a) the nucleotide sequence shown in SEQ ID NO. 1 or (b) a nucleotide sequence corresponding to the nucleotide sequence (a) within the scope of the degeneracy of the genetic code.
摘要翻译： 本发明涉及分离重组核心链霉抗生物素蛋白的方法，其中宿主细胞用编码核心链霉抗生物素蛋白的DNA转化，转化的宿主细胞在合适的条件下培养，编码核心链霉抗生物素蛋白的DNA被表达，重组核心链霉亲和素 从宿主细胞或培养基中分离，其中使用编码核心链亲和素的DNA，其具有（a）SEQ ID NO： 1或（b）对应于遗传密码简并范围内的核苷酸序列（a）的核苷酸序列。
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公开(公告)号：US08877895B2
公开(公告)日：2014-11-04
申请号：US13217537
申请日：2011-08-25
CPC分类号： C12N15/62 , C07K14/775 , C07K2319/02 , C07K2319/21 , C07K2319/50 , C07K2319/70 , C12N15/70 , C12P21/02 , C12P21/06
摘要： A pro-polypeptide which is useful for the expression of a polypeptide of interest in a prokaryotic cell. Therefore the pro-polypeptide is fused to the N-terminus of the polypeptide of interest. The pro-polypeptide as reported herein provides for improved expression yields and improves the handling of the fusion polypeptide (downstream processing, purification). For example, efficient endotoxin removal is effected while the protein of interest comprising the pro-polypeptide is bound e.g. to an affinity chromatography material. Thereafter the pro-polypeptide can efficiently be cleaved from the polypeptide of interest by the incorporated protease cleavage site with the cognate protease.
摘要翻译： 可用于在原核细胞中表达目的多肽的原多肽。 因此，原多肽与感兴趣的多肽的N末端融合。 本文报道的前多肽提供改善的表达产率并改善融合多肽的处理（下游处理，纯化）。 例如，有效的内毒素去除被实现，而包含前多肽的目的蛋白质被结合例如。 至亲和层析材料。 此后，通过与同源蛋白酶合并的蛋白酶切割位点，可以有效地从所述多肽切割所述多肽。
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公开(公告)号：US08501439B2
公开(公告)日：2013-08-06
申请号：US12765229
申请日：2010-04-22
IPC分类号： C12N15/18
CPC分类号： C07K14/48
摘要： The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.
摘要翻译： 本发明涉及从前体proNGF生产生物活性β-NGF的方法。 在原核宿主细胞中表达β-NGF的形式后，以不溶性无活性聚集体（包涵体）的形式分离重组蛋白。 在将其溶解在强变性剂中并随后转化成通过存在于天然β-NGF中的二硫键确定的天然构象后，通过随后分离原序列获得生物活性β-NGF。
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公开(公告)号：US08318671B1
公开(公告)日：2012-11-27
申请号：US09807096
申请日：1999-10-11
CPC分类号： C07K14/48
摘要： The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.
摘要翻译： 本发明涉及从前体proNGF生产生物活性的NGF的方法。 在原核宿主细胞中表达生物素-NGF的形式后，重组蛋白以不溶性非活性聚集体（包涵体）的形式分离。 在将其溶解在强变性剂中并随后转化成通过存在于天然和/或生长因子中的二硫键确定的天然构象后，通过随后分离原序列获得生物活性 。
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公开(公告)号：US20120214200A1
公开(公告)日：2012-08-23
申请号：US13217537
申请日：2011-08-25
CPC分类号： C12N15/62 , C07K14/775 , C07K2319/02 , C07K2319/21 , C07K2319/50 , C07K2319/70 , C12N15/70 , C12P21/02 , C12P21/06
摘要： A pro-polypeptide which is useful for the expression of a polypeptide of interest in a prokaryotic cell. Therefore the pro-polypeptide is fused to the N-terminus of the polypeptide of interest. The pro-polypeptide as reported herein provides for improved expression yields and improves the handling of the fusion polypeptide (downstream processing, purification). For example, efficient endotoxin removal is effected while the protein of interest comprising the pro-polypeptide is bound e.g. to an affinity chromatography material. Thereafter the pro-polypeptide can efficiently be cleaved from the polypeptide of interest by the incorporated protease cleavage site with the cognate protease.
摘要翻译： 可用于在原核细胞中表达目的多肽的原多肽。 因此，原多肽与感兴趣的多肽的N末端融合。 本文报道的前多肽提供改善的表达产率并改善融合多肽的处理（下游处理，纯化）。 例如，有效的内毒素去除被实现，而包含前多肽的目的蛋白质被结合例如。 至亲和层析材料。 此后，通过与同源蛋白酶合并的蛋白酶切割位点，可以有效地从所述多肽切割所述多肽。
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公开(公告)号：US20070225205A1
公开(公告)日：2007-09-27
申请号：US11532045
申请日：2006-09-14
申请人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
发明人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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18.发明申请METHOD FOR PRODUCING A LIPID PARTICLE, THE LIPID PARTICLE ITSELF AND ITS USE 审中-公开生产脂肪颗粒的方法，脂质颗粒及其用途公开(公告)号：US20120190609A1
公开(公告)日：2012-07-26
申请号：US13217536
申请日：2011-08-25
申请人： Martin Bader , Monika Baehner , Adelbert Grossmann , Anton Jochner , Hubert Kettenberger , Silke Mohl
发明人： Martin Bader , Monika Baehner , Adelbert Grossmann , Anton Jochner , Hubert Kettenberger , Silke Mohl
CPC分类号： A61K9/1275 , A61K9/1277 , A61K38/1709 , C07K5/1008 , C07K14/47 , C07K14/775 , C07K2319/00
摘要： A method for producing a lipid particle comprising the following: i) providing a first solution comprising denatured apolipoprotein, ii) adding the first solution to a second solution comprising at least two lipids and a detergent but no apolipoprotein, and iii) removing the detergent from the solution obtained in ii) and thereby producing a lipid particle.
摘要翻译： 一种制备脂质颗粒的方法，包括以下步骤：i）提供包含变性载脂蛋白的第一溶液，ii）将第一溶液加入到包含至少两种脂质和洗涤剂但不含载脂蛋白的第二溶液中，和iii）将去污剂从 在ii）中获得的溶液，从而产生脂质颗粒。
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公开(公告)号：US20080171363A1
公开(公告)日：2008-07-17
申请号：US11838730
申请日：2007-08-14
申请人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
发明人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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公开(公告)号：US20080076710A1
公开(公告)日：2008-03-27
申请号：US11744424
申请日：2007-05-04
申请人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
发明人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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