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    Method for Synthesizing Nucleic Acids, and Application Thereof
    16.
    发明申请
    Method for Synthesizing Nucleic Acids, and Application Thereof 审中-公开
    合成核酸的方法及其应用

    公开(公告)号：US20110027794A1

    公开(公告)日：2011-02-03

    申请号：US12905910

    申请日：2010-10-15

    申请人： Sven Klussmann ,  Florian Jarosch ,  Axel Vater

    发明人： Sven Klussmann ,  Florian Jarosch ,  Axel Vater

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12N15/102 ,  C12N15/1096 ,  C12P19/34

    摘要： The invention relates to a method for synthesizing a nucleic acid containing modified nucleotides. The method encompasses the following steps: a matrix strand is provided; —a primer which at least partially hybridizes on the matrix strand is provided; —nucleoside triphosphates, at least some of which are modified nucleoside triphosphates, are provided; —a polymerase activity is supplied; and—the matrix strand, the primer, and the nucleoside triphosphates are incubated so as to synthesize a nucleic acid that is substantially complementary to the matrix strand. The polymerase activity can be a reverse transcriptase activity.

    摘要翻译： 本发明涉及一种合成含有修饰核苷酸的核酸的方法。 该方法包括以下步骤：提供矩阵线; 提供了在基质链上至少部分杂交的引物; 核苷三磷酸盐，其中至少一些是修饰的核苷三磷酸酯; - 提供聚合酶活性; 并且将基质链，引物和核苷三磷酸盐孵育以合成基本上与基质链互补的核酸。 聚合酶活性可以是逆转录酶活性。

    Method for the Synthesizing Nucleic Acids, and Application Thereof
    17.
    发明申请
    Method for the Synthesizing Nucleic Acids, and Application Thereof 审中-公开
    合成核酸的方法及其应用

    公开(公告)号：US20080070238A1

    公开(公告)日：2008-03-20

    申请号：US10572291

    申请日：2004-09-14

    申请人： Sven Klussmann ,  Florian Jarosch ,  Axel Vater

    发明人： Sven Klussmann ,  Florian Jarosch ,  Axel Vater

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12N15/102 ,  C12N15/1096 ,  C12P19/34

    摘要： The invention relates to a method for synthesizing a nucleic acid comprising modified nucleotides. Said method encompasses the following steps: a matrix strand is provided; —a primer which at least partially hybridizes on the matrix strand is provided; —nucleoside triphosphates, at least some of which are modified nucleoside triphosphates, are provided; —a polymerase activity is supplied; and —the matrix strand, the primer, and the nucleoside triphosphates are incubated so as to synthesize a nucleic acid that is substantially complementary to the matrix strand. The inventive method is characterized in that the polymerase activity represents a reverse transcriptase activity.

    摘要翻译： 本发明涉及一种合成含有修饰核苷酸的核酸的方法。 所述方法包括以下步骤：提供矩阵线; 提供了在基质链上至少部分杂交的引物; 核苷三磷酸盐，其中至少一些是修饰的核苷三磷酸酯; - 提供聚合酶活性; 并且 - 将基质链，引物和核苷三磷酸盐孵育以合成基本上与基质链互补的核酸。 本发明的方法的特征在于聚合酶活性代表逆转录酶活性。