公开(公告)号：US10104307B2

公开(公告)日：2018-10-16

申请号：US15667435

申请日：2017-08-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Steve Swihart ,  Evan Thrush ,  Boaz Ran

IPC: H04N7/12 ,  H04N11/02 ,  H04N11/04 ,  H04N5/235 ,  H04N5/355 ,  H04N5/361 ,  H04N5/365 ,  G01N21/76 ,  G01N21/64 ,  G01N21/27

Abstract: Methods and digital imaging devices disclosed herein are adapted to capture images of a specimen in a chemical reaction using a series of short exposures of light emissions from the specimen over a period of time. The series of short exposures is captured using an array of pixels of an image sensor in the digital imaging device that are configured for performing continuous non-destructive read operations to read out a set of non-destructive read images of the specimen from the pixel array. In one embodiment, images are captured by delaying the read out until at or near the end of the chemical reaction to reduce read noise in the images. The signals read out from the image sensor can be continuously monitored and the capturing of images can be discontinued either automatically or based on a command from a user. The captured images can then be displayed in a graphical display.

公开(公告)号：US09933565B2

公开(公告)日：2018-04-03

申请号：US15211728

申请日：2016-07-15

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Steve Swihart ,  Evan Thrush ,  Yochanan Uri ,  Boaz Ran ,  Itay Barak ,  Kevin McDonald

IPC: G02B6/08 ,  G02B5/22 ,  G01N21/64 ,  G02B5/28 ,  G01N27/447

CPC classification number: G02B6/08 ,  G01N21/6454 ,  G01N27/44721 ,  G01N2021/6484 ,  G02B5/22 ,  G02B5/28

Abstract: Devices, systems, methods, and kits for contact imaging are provided. A contact imaging device includes an imaging sensor, a fixed fiber faceplate mechanically coupled to the imaging sensor, and an optical filtering layer mechanically coupled to the fixed fiber faceplate. The optical filtering layer can include an interference filter, an absorptive filter, and/or a removable fiber faceplate. The contact imaging device can be used to image fluorescent samples by filtering out excitation light on the basis of wavelength and/or angle of incidence.

公开(公告)号：US09841378B2

公开(公告)日：2017-12-12

申请号：US14699755

申请日：2015-04-29

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Evan Thrush ,  Steve Swihart ,  Neeraj Bhatt

IPC: G01N21/64

CPC classification number: G01N21/6456 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2021/6482 ,  G01N2201/062 ,  G01N2201/0627 ,  G01N2201/0638 ,  G01N2201/12

Abstract: An imaging assembly and processing system that includes a sample platform having a target region which can hold a sample, where the sample can be marked with fluorescent or phosphorescent markers. The imaging assembly can have an excitation light module proximate to the sample platform that emits light to excite the markers, and a lens module positioned to receive emission light from excited markers in target region. At least one series filter assembly or interference filter can be arranged in front of, behind, or both in front of and behind the lens module. The assembly includes a light sensor and a processor and imaging module configured to process data captured by the light sensor. Images of the sample are generated based on the emission light from the sample that transmit through and are filtered by the lens assembly and series filter assembly or interference filter.

公开(公告)号：US09774804B2

公开(公告)日：2017-09-26

申请号：US14563411

申请日：2014-12-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Boaz Ran ,  Steve Swihart ,  Evan Thrush ,  Itay Barak

IPC: H04N5/361 ,  H04N5/365

CPC classification number: H04N5/361 ,  H04N5/365

Abstract: In one application, an imaging device includes an image sensor having an array of pixels, and a mask coupled with the image sensor. The mask is configured to darken a plurality of isolated pixels or groups of pixels interspersed within the array of pixels. The imaging device also includes a processor coupled with the image sensor and configured to receive image data from the image sensor, and determine a dark current fixed pattern noise based on the image data received from the plurality of darkened pixels or groups of pixels.

公开(公告)号：US20150172526A1

公开(公告)日：2015-06-18

申请号：US14563355

申请日：2014-12-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Steve Swihart ,  Evan Thrush ,  Boaz Ran

IPC: H04N5/235 ,  H04N5/357 ,  H04N5/374 ,  H04N5/359 ,  H04N5/361 ,  H04N5/232

CPC classification number: H04N5/2355 ,  G01N21/272 ,  G01N21/6408 ,  G01N21/6456 ,  G01N21/76 ,  H04N5/2173 ,  H04N5/2353 ,  H04N5/35545 ,  H04N5/361 ,  H04N5/365

Abstract: Methods and digital imaging devices disclosed herein are adapted to capture images of a specimen in a chemical reaction using a series of short exposures of light emissions from the specimen over a period of time. The series of short exposures is captured using an array of pixels of an image sensor in the digital imaging device that are configured for performing continuous non-destructive read operations to read out a set of non-destructive read images of the specimen from the pixel array. In one embodiment, images are captured by delaying the read out until at or near the end of the chemical reaction to reduce read noise in the images. The signals read out from the image sensor can be continuously monitored and the capturing of images can be discontinued either automatically or based on a command from a user. The captured images can then be displayed in a graphical display.

Abstract translation: 本文公开的方法和数字成像装置适于在一段时间内使用来自样品的一系列短的曝光来捕获化学反应中的样本的图像。 使用数字成像设备中的图像传感器的像素阵列来捕获一系列短曝光，其被配置为执行连续的非破坏性读取操作，以从像素阵列读出样本的一组非破坏性读取图像 。 在一个实施例中，通过延迟读出直到化学反应结束或接近化学反应结束来捕获图像，以减少图像中的读取噪声。 可以连续地监视从图像传感器读出的信号，并且可以自动地或基于来自用户的命令中断图像的捕获。 然后可以在图形显示中显示捕获的图像。