公开(公告)号：US20170198306A1

公开(公告)日：2017-07-13

申请号：US15127128

申请日：2015-03-23

Applicant: CELLECTIS

Inventor： Julien Valton ,  Philippe Duchateau

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N5/0783 ,  C12N15/85 ,  C12N15/86 ,  C12N5/074

CPC classification number: C12N15/907 ,  C12N5/0607 ,  C12N5/0636 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/8509 ,  C12N15/86 ,  C12N15/90 ,  C12N2310/14 ,  C12N2310/20 ,  C12N2320/30 ,  C12N2510/00 ,  C12N2517/02 ,  C12N2740/15043 ,  C12Y301/26

Abstract: This invention relates to materials and methods for gene editing in mammalian cells, and more particularly to methods for gene editing using DNA-guided Argonaute (Ago) interference systems (DAIS) in T-cells.

公开(公告)号：US20160298098A1

公开(公告)日：2016-10-13

申请号：US15069672

申请日：2016-03-14

Applicant: CELLECTIS, S.A.

Inventor： Philippe Duchateau ,  Julien Valton ,  Claudia Bertonati ,  Jean-Charles Epinat ,  George H. Silva ,  Alexandre Juillerat ,  Marine Beurdeley

IPC: C12N9/22 ,  C07K14/47 ,  C12P19/34

CPC classification number: C12N9/16 ,  A61K38/00 ,  C07K14/4703 ,  C07K2319/00 ,  C07K2319/80 ,  C12N9/22 ,  C12P19/34

Abstract: The present invention relates to a method for the generation of compact Transcription Activator-Like Effector Nucleases (TALENS) that can efficiently target and process double-stranded DNA. More specifically, the present invention concerns a method for the creation of TALENs that consist of a single TALE DNA binding domain fused to at least one catalytic domain such that the active entity is composed of a singe polypeptide chain for simple and efficient vectorization and does not require dimerization to target a specific single double-stranded DNA tar et sequence of interest and process DNA nearby said DNA target sequence. The present invention also relates to compact TALENs, vectors, compositions and kits used to implement the method.

Abstract translation: 本发明涉及能够有效靶向和加工双链DNA的致密转录激活子样效应核酸酶（TALENS）的生成方法。 更具体地，本发明涉及一种用于产生TALEN的方法，其由与至少一个催化结构域融合的单个TALE DNA结合结构域组成，使得活性实体由简单且有效的载体化的单个多肽链组成，并且不 需要二聚化来靶向目标特定的单链双链DNA焦油和序列，并在DNA附近处理DNA靶序列。 本发明还涉及用于实施该方法的紧凑型TALEN，载体，组合物和试剂盒。

公开(公告)号：US20140230083A1

公开(公告)日：2014-08-14

申请号：US13949880

申请日：2013-07-24

Applicant: CELLECTIS

Inventor： Claudia Bertonati ,  Philippe Duchateau ,  Alexandre Juillerat ,  George Silva ,  Julien Valton

IPC: C12N15/87 ,  A01K67/027

CPC classification number: C07K14/195 ,  A01K67/0275 ,  A01K2217/07 ,  C07K2319/80 ,  C12N9/22 ,  C12N15/62 ,  C12N15/87 ,  C12N15/902 ,  C12N15/907 ,  C12Y301/21

Abstract: The present invention concerns new modular base-per-base specific nucleic acid binding domains (MBBBD) derived from newly identified proteins from the bacterial endosymbiont Burkholderia Rhizoxinica and their use for engineering nucleic acid processing enzymes, such as specific endonucleases or transcription activators.

Abstract translation: 本发明涉及从新鉴定的来自细菌内分泌杆菌伯克霍尔德杆菌（Burkholderia Rhizoxinica）的新鉴定的蛋白质中获得的新的基于碱基的碱基特异性核酸结合结构域（MBBBD）及其用于工程化核酸加工酶如特异性内切核酸酶或转录激活子的用途。

公开(公告)号：US12221478B2

公开(公告)日：2025-02-11

申请号：US16755093

申请日：2018-04-16

Applicant: CELLECTIS

Inventor： Brian Busser ,  Philippe Duchateau ,  Alexandre Juillerat ,  Laurent Poirot ,  Julien Valton ,  Mohit Sachdeva

IPC: C07K16/28 ,  A61K39/00 ,  C07K16/30 ,  C12N5/0783 ,  C12N5/10

Abstract: The invention pertains to the field of adaptive cell immunotherapy. It provides with the genetic insertion of exogenous coding sequence(s) into genetically engineered immune cells to prevent cytokine release syndrome to arise during the course of cell therapy. These exogenous coding sequences are more particularly soluble human polypeptides placed under the transcriptional control of endogenous gene promoters that are sensitive to immune cells activation. Such method allows the production of safer immune primary cells of higher therapeutic potential.

公开(公告)号：US20220010292A1

公开(公告)日：2022-01-13

申请号：US17489454

申请日：2021-09-29

Applicant: CELLECTIS

Inventor： Philippe Duchateau ,  Julien Valton ,  Claudia Bertonati ,  Jean-Charles Epinat ,  George H. Silva ,  Alexandre Juillerat ,  Marine Beurdeley

IPC: C12N9/16 ,  C12N15/63 ,  C07K14/195 ,  C12N5/071 ,  C12N5/10 ,  C12N9/22

Abstract: The present invention relates to a method for the generation of compact Transcription Activator-Like Effector Nucleases (TALENs) that can efficiently target and process double-stranded DNA. More specifically, the present invention concerns a method for the creation of TALENs that consist of a single TALE DNA binding domain fused to at least one catalytic domain such that the active entity is composed of a single polypeptide chain for simple and efficient vectorization and does not require dimerization to target a specific single double-stranded DNA target sequence of interest and process DNA nearby the DNA target sequence. The present invention also relates to compact TALENs, vectors, compositions and kits used to implement the method.

公开(公告)号：US11220683B2

公开(公告)日：2022-01-11

申请号：US16379073

申请日：2019-04-09

Applicant: CELLECTIS

Inventor： Philippe Duchateau ,  Julien Valton

IPC: C12N15/10 ,  C12N15/85 ,  C07K14/47 ,  C12N9/22 ,  G01N33/53

Abstract: The present invention relates to polypeptides and more particularly to Transcription Activator-Like Effector derived proteins that allow to efficiently target and/or process nucleic acids. Particularly, the present invention reports the characterization of TALE derived proteins that can efficiently target methylated DNA. The present invention more specifically relates to TALE derived proteins that allow activation of methylated promoters responsible for gene silencing.

公开(公告)号：US11186824B2

公开(公告)日：2021-11-30

申请号：US15556558

申请日：2016-03-11

Applicant: Cellectis

Inventor： Philippe Duchateau ,  Jean-Pierre Cabaniols ,  Julien Valton ,  Laurent Poirot

IPC: A61K35/17 ,  A61K48/00 ,  C12N5/0783 ,  C12N5/00 ,  C07K14/005 ,  C07K14/705 ,  C07K14/74 ,  C07K16/28 ,  C12N9/22 ,  C12N15/11 ,  A61K39/00

Abstract: The present invention relates to methods for developing engineered immune cells such as T-cells for immunotherapy that have a higher potential of persistence and/or engraftment in host organism. IN particular, this method involves an inactivation of at least one gene involved in self/non self recognition, combined with a step of contact with at least one non-endogenous immunosuppressive polypeptide. The invention allows the possibility for a standard and affordable adoptive immunotherapy, whereby the risk of GvH is reduced.

公开(公告)号：US10894093B2

公开(公告)日：2021-01-19

申请号：US16092417

申请日：2017-04-13

Applicant: CELLECTIS

Inventor： Julien Valton ,  Veronique Zennou ,  Philippe Duchateau ,  Laurent Poirot

IPC: C12N5/0783 ,  C12N15/113 ,  A61P31/00 ,  A61K48/00 ,  A61K35/17 ,  C07K14/725 ,  A61P35/00

Abstract: The present invention relates to therapeutic cells for immunotherapy to treat patients with cancer. In particular, the inventors develop a method of engineering drug-specific hypersensitive T-cell, which can be depleted in vivo by the administration of said specific drug in case of occurrence of a serious adverse even. The invention opens the way to standard and affordable adoptive immunotherapy strategies for treating cancer.

公开(公告)号：US10526406B2

公开(公告)日：2020-01-07

申请号：US15111441

申请日：2015-01-14

Applicant: CELLECTIS

Inventor： Philippe Duchateau ,  Julien Valton

IPC: A61K35/17 ,  C07K16/28 ,  C07K14/725 ,  C12N5/0783 ,  C07K14/705 ,  A61K38/00

Abstract: The present invention relates to a new generation of chimeric antigen receptors (CAR), under single-chain or multi-chain forms, the specificity of which, to a desired antigen, is conferred by a VNAR polypeptide derived from monomeric antibodies from cartilaginous fish. Such CARs, which aim to redirect immune cell specificity toward selected undesired malignant cells, are compact and thus particularly adapted to target hollow antigens such as ions channels of efflux pumps present at the surface of drug-resistant cells. The invention encompasses the polynucleotides, vectors encoding said multi-chain CAR and the isolated cells expressing them at their surface, in particularly for their use in immunotherapy.

公开(公告)号：US10472396B2

公开(公告)日：2019-11-12

申请号：US14696920

申请日：2015-04-27

Applicant: CELLECTIS

Inventor： Claudia Bertonati ,  Philippe Duchateau ,  Alexandre Juillerat ,  George Silva ,  Julien Valton

IPC: C12N9/22 ,  C12N15/62 ,  C07K14/195 ,  A01K67/027 ,  C12N15/87 ,  C12N15/90

Abstract: The present invention concerns new modular base-per-base specific nucleic acid binding domains (MBBBD) derived from newly identified proteins from the bacterial endosymbiont Burkholderia Rhizoxinica and their use for engineering nucleic acid processing enzymes, such as specific endonucleases or transcription activators.