-
11.发明授权Complexes comprising a prion protein and a peptidyl prolyl isomerase chaperone, and method for producing and using them 有权包含朊病毒蛋白和肽基脯氨酰异构酶伴侣的复合物及其制备和使用方法公开(公告)号：US07094757B2
公开(公告)日：2006-08-22
申请号：US10745393
申请日：2003-12-23
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20 , G01N2800/2821 , Y02A50/472
摘要： The present invention relates to the diagnosis of transmissible spongiform encephalopathies (TSEs). It especially teaches the production of a soluble prion protein (PrP)-chaperone complex and the advantageous use of such chaperone-PrP complex, especially in the detection of PrP in an immunoassay, as well as its use as and immunogen.
摘要翻译： 本发明涉及传染性海绵状脑病（TSEs）的诊断。 它特别教导了可溶性朊病毒蛋白（PrP） - 辅助复合物的生产和这种伴侣 - PrP复合物的有利使用，特别是在免疫测定中检测PrP以及其作为免疫原的用途。
-
公开(公告)号：US08790659B2
公开(公告)日：2014-07-29
申请号：US13569353
申请日：2012-08-08
IPC分类号： A61K39/245
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译： 公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBP）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞 。 还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。
-
13.发明授权Soluble complexes of amylod β peptide and peptidyl prolyl isomerase chaperone and methods of making and using them 有权淀粉状β肽和肽基脯氨酰异构酶伴侣的可溶性复合物及其制备和使用方法公开(公告)号：US07094884B2
公开(公告)日：2006-08-22
申请号：US10443654
申请日：2003-05-22
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20 , G01N2800/2821 , Y02A50/472
摘要： The present invention relates to the diagnosis of Alzheimer's Disease. It especially teaches the production of a soluble Aβ-chaperone complex and the advantageous use of such chaperone-Aβ complex, especially in the detection of Aβ in an immunoassay, as well as its use as an immunogen.
摘要翻译： 本发明涉及阿尔茨海默病的诊断。 它特别教导了可溶性的Abeta-伴侣复合物的生产和这种伴侣 - Aβ复合物的有利使用，特别是在免疫测定中检测Abeta以及其作为免疫原的用途。
-
14.发明申请SOLUBLE COMPLEXES OF AMYLOD BETA PEPTIDE AND PEPTIDYL PROLYL ISOMERASE CHAPERONE AND METHODS OF MAKING AND USING THEM 有权氨基酸肽和磷脂酰胆碱异构酶的可溶性复合物及其制备和使用方法公开(公告)号：US20050112720A1
公开(公告)日：2005-05-26
申请号：US10443654
申请日：2003-05-22
IPC分类号： A61K39/00 , A61K47/48 , C07K14/16 , C12N9/90 , G01N33/543 , G01N33/569 , C12P21/06
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20 , G01N2800/2821 , Y02A50/472
摘要： The present invention relates to the diagnosis of Alzheimer's Disease. It especially teaches the production of a soluble Aβ-chaperone complex and the advantageous use of such chaperone-Aβ complex, especially in the detection of Aβ in an immunoassay, as well as its use as an immunogen.
摘要翻译： 本发明涉及阿尔茨海默病的诊断。 它特别教导了可溶性的Abeta-伴侣复合物的生产和这种伴侣 - Aβ复合物的有利使用，特别是在免疫测定中检测Abeta以及其作为免疫原的用途。
-
公开(公告)号：US20120308994A1
公开(公告)日：2012-12-06
申请号：US13569353
申请日：2012-08-08
IPC分类号： G01N21/76 , G01N33/569
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译： 公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBP）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞 。 还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。
-
16.发明申请FUSION PROTEIN COMPRISING AN E. COLI CHAPERONE PROTEIN AND A HUMAN CHAPERONE PROTEIN 有权包含大肠杆菌蛋白和蛋白质的融合蛋白公开(公告)号：US20110189754A1
公开(公告)日：2011-08-04
申请号：US13083903
申请日：2011-04-11
CPC分类号： C12N9/90 , C07K2319/35
摘要： The invention discloses the cloning, expression and uses of a chimeric fusion protein with superior chaperone and folding activities compared to the wild type chaperones. This invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule containing nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for an FK506 binding protein (FKBP) or an FK506-binding-protein-like domain (FKBP-like domain). In particular, this invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule containing nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for a human FKBP type peptidyl-prolyl-cis/trans isomerase (PPIase), methods of producing these chimeric fusion proteins and their uses as folding helpers in the production of other proteins and in the process of the production of vaccines or pharmaceuticals, and as folding helpers for performing immunoassays.
摘要翻译： 本发明公开了与野生型伴侣相比，具有优异的伴侣和折叠活性的嵌合融合蛋白的克隆，表达和用途。 本发明涉及由含有编码非人伴侣蛋白多肽结合片段的核苷酸序列的重组DNA分子编码的嵌合融合蛋白和编码FK506结合蛋白（FKBP）或FK506-结合蛋白 - 像域（FKBP样域）。 特别地，本发明涉及由包含编码非人伴侣蛋白多肽结合片段的核苷酸序列的重组DNA分子编码的嵌合融合蛋白和编码人FKBP型肽基 - 脯氨酰 - 顺/反异构酶的核苷酸序列 （PPIase），生产这些嵌合融合蛋白的方法及其在生产其他蛋白质和疫苗或药物生产过程中作为折叠助剂的用途，以及用于进行免疫测定的折叠助剂。
-
公开(公告)号：US20090042212A1
公开(公告)日：2009-02-12
申请号：US12104751
申请日：2008-04-17
IPC分类号： G01N33/53 , C07K7/00 , C07K14/00 , C12N15/11 , C12N15/00 , C12N1/20 , C12P21/04 , G01N33/566
CPC分类号： G01N33/6854 , C07K14/20 , C07K14/245 , C07K14/45 , C07K14/47 , C07K2319/70 , C12N15/62 , C12N2710/16122 , C12N2710/16134 , G01N2333/02 , G01N2333/035 , G01N2333/04 , G01N2333/045 , G01N2333/05 , G01N2333/19 , G01N2333/45
摘要： The present invention relates to fusion proteins: suitable as test antigens in the detection of infections with pathogens, particularly of primary infections with pathogens. Further, the invention relates to methods for detecting and differentially determining antibodies, particularly IgM antibodies resulting from an infection with a pathogenic organism. Furthermore, test reagents for carrying out these methods are provided.
摘要翻译： 本发明涉及融合蛋白：适合作为检测病原体感染的试验抗原，特别是病原体的初次感染。 此外，本发明涉及用于检测和差异测定抗体的方法，特别是由致病性生物体的感染产生的IgM抗体。 此外，提供了用于实施这些方法的测试试剂。
-
公开(公告)号：US08263086B2
公开(公告)日：2012-09-11
申请号：US12436396
申请日：2009-05-06
IPC分类号： A61K39/245
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译： 公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBPs）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞 。 还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。
-
19.发明授权Fusion protein comprising an Escherichia coli chaperone protein and a human chaperone protein 有权包含大肠杆菌伴侣蛋白和人伴侣蛋白的融合蛋白公开(公告)号：US08247206B2
公开(公告)日：2012-08-21
申请号：US13083903
申请日：2011-04-11
CPC分类号： C12N9/90 , C07K2319/35
摘要： The invention discloses the cloning, expression and uses of a chimeric fusion protein with superior chaperone and folding activities compared to the wild type chaperones. This invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule containing nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for an FK506 binding protein (FKBP) or an FK506-binding-protein-like domain (FKBP-like domain). In particular, this invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule containing nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for a human FKBP type peptidyl-prolyl-cis/trans isomerase (PPIase), methods of producing these chimeric fusion proteins and their uses as folding helpers in the production of other proteins and in the process of the production of vaccines or pharmaceuticals, and as folding helpers for performing immunoassays.
摘要翻译： 本发明公开了与野生型伴侣相比，具有优异的伴侣和折叠活性的嵌合融合蛋白的克隆，表达和用途。 本发明涉及由含有编码非人伴侣蛋白多肽结合片段的核苷酸序列的重组DNA分子编码的嵌合融合蛋白和编码FK506结合蛋白（FKBP）或FK506-结合蛋白 - 像域（FKBP样域）。 特别地，本发明涉及由包含编码非人伴侣蛋白多肽结合片段的核苷酸序列的重组DNA分子编码的嵌合融合蛋白和编码人FKBP型肽基 - 脯氨酰 - 顺/反异构酶的核苷酸序列 （PPIase），生产这些嵌合融合蛋白的方法及其在生产其他蛋白质和疫苗或药物生产过程中作为折叠助剂的用途，以及用于进行免疫测定的折叠助剂。
-
公开(公告)号：US20090291892A1
公开(公告)日：2009-11-26
申请号：US12436396
申请日：2009-05-06
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译： 公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBP）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞 。 还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。
-
-
-
-
-
-
-
-
-
搜索结果
31 条记录 (耗时 0.029 秒)
