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公开(公告)号：US08790659B2
公开(公告)日：2014-07-29
申请号：US13569353
申请日：2012-08-08
IPC分类号： A61K39/245
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译： 公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBP）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞 。 还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。
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公开(公告)号：US08426167B2
公开(公告)日：2013-04-23
申请号：US11786847
申请日：2007-04-13
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
IPC分类号： C12P21/04
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria, such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor; methods of recombinant protein expression, the recombinant polypeptides thus obtained, as well as to the use of such polypeptides.
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3.发明授权Fusion protein comprising an E. coli chaperone protein and a human chaperone protein 有权包含大肠杆菌伴侣蛋白和人伴侣蛋白的融合蛋白公开(公告)号：US07947494B2
公开(公告)日：2011-05-24
申请号：US12166574
申请日：2008-07-02
CPC分类号： C12N9/90 , C07K2319/35
摘要： The invention discloses the cloning, expression and uses of a chimeric fusion protein with superior chaperone and folding activities compared to the wild type chaperones. This invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for an FK506 binding protein (FKBP) or an FK506-binding-protein-like domain (FKBP-like domain). In particular, this invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for a human FKBP type peptidyl-prolyl-cis/trans isomerase (PPIase), methods of producing these chimeric fusion proteins and their uses as folding helpers in the production of other proteins and in the process of the production of vaccines or pharmaceuticals, and as folding helpers for performing immunoassays.
摘要翻译： 本发明公开了与野生型伴侣相比，具有优异的伴侣和折叠活性的嵌合融合蛋白的克隆，表达和用途。 本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码FK506结合蛋白（FKBP）或FK506-结合蛋白 - 像域（FKBP样域）。 特别地，本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码人FKBP型肽基 - 脯氨酰 - 顺/反异构酶的核苷酸序列 （PPIase），生产这些嵌合融合蛋白的方法及其在生产其他蛋白质和疫苗或药物生产过程中作为折叠助剂的用途，以及用于进行免疫测定的折叠助剂。
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公开(公告)号：US20120308994A1
公开(公告)日：2012-12-06
申请号：US13569353
申请日：2012-08-08
IPC分类号： G01N21/76 , G01N33/569
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译： 公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBP）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞 。 还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。
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5.发明申请FUSION PROTEIN COMPRISING AN E. COLI CHAPERONE PROTEIN AND A HUMAN CHAPERONE PROTEIN 有权包含大肠杆菌蛋白和蛋白质的融合蛋白公开(公告)号：US20110189754A1
公开(公告)日：2011-08-04
申请号：US13083903
申请日：2011-04-11
CPC分类号： C12N9/90 , C07K2319/35
摘要： The invention discloses the cloning, expression and uses of a chimeric fusion protein with superior chaperone and folding activities compared to the wild type chaperones. This invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule containing nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for an FK506 binding protein (FKBP) or an FK506-binding-protein-like domain (FKBP-like domain). In particular, this invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule containing nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for a human FKBP type peptidyl-prolyl-cis/trans isomerase (PPIase), methods of producing these chimeric fusion proteins and their uses as folding helpers in the production of other proteins and in the process of the production of vaccines or pharmaceuticals, and as folding helpers for performing immunoassays.
摘要翻译： 本发明公开了与野生型伴侣相比，具有优异的伴侣和折叠活性的嵌合融合蛋白的克隆，表达和用途。 本发明涉及由含有编码非人伴侣蛋白多肽结合片段的核苷酸序列的重组DNA分子编码的嵌合融合蛋白和编码FK506结合蛋白（FKBP）或FK506-结合蛋白 - 像域（FKBP样域）。 特别地，本发明涉及由包含编码非人伴侣蛋白多肽结合片段的核苷酸序列的重组DNA分子编码的嵌合融合蛋白和编码人FKBP型肽基 - 脯氨酰 - 顺/反异构酶的核苷酸序列 （PPIase），生产这些嵌合融合蛋白的方法及其在生产其他蛋白质和疫苗或药物生产过程中作为折叠助剂的用途，以及用于进行免疫测定的折叠助剂。
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公开(公告)号：US20090042212A1
公开(公告)日：2009-02-12
申请号：US12104751
申请日：2008-04-17
IPC分类号： G01N33/53 , C07K7/00 , C07K14/00 , C12N15/11 , C12N15/00 , C12N1/20 , C12P21/04 , G01N33/566
CPC分类号： G01N33/6854 , C07K14/20 , C07K14/245 , C07K14/45 , C07K14/47 , C07K2319/70 , C12N15/62 , C12N2710/16122 , C12N2710/16134 , G01N2333/02 , G01N2333/035 , G01N2333/04 , G01N2333/045 , G01N2333/05 , G01N2333/19 , G01N2333/45
摘要： The present invention relates to fusion proteins: suitable as test antigens in the detection of infections with pathogens, particularly of primary infections with pathogens. Further, the invention relates to methods for detecting and differentially determining antibodies, particularly IgM antibodies resulting from an infection with a pathogenic organism. Furthermore, test reagents for carrying out these methods are provided.
摘要翻译： 本发明涉及融合蛋白：适合作为检测病原体感染的试验抗原，特别是病原体的初次感染。 此外，本发明涉及用于检测和差异测定抗体的方法，特别是由致病性生物体的感染产生的IgM抗体。 此外，提供了用于实施这些方法的测试试剂。
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7.发明申请Methods for producing fusion polypeptides or enhancing expression of fusion polypeptides 有权用于产生融合多肽或增强融合多肽表达的方法公开(公告)号：US20090028893A1
公开(公告)日：2009-01-29
申请号：US11786847
申请日：2007-04-13
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria, such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor; methods of recombinant protein expression, the recombinant polypeptides thus obtained, as well as to the use of such polypeptides.
摘要翻译： 本发明涉及在细菌如大肠杆菌中克隆和表达外源蛋白质或多肽。 特别地，本发明涉及包含选自FkpA，SlyD和触发因子的FKBP型肽基脯氨酰异构酶的表达工具; 重组蛋白表达的方法，如此获得的重组多肽，以及这些多肽的用途。
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公开(公告)号：US08263086B2
公开(公告)日：2012-09-11
申请号：US12436396
申请日：2009-05-06
IPC分类号： A61K39/245
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译： 公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBPs）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞 。 还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。
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9.发明授权Fusion protein comprising an Escherichia coli chaperone protein and a human chaperone protein 有权包含大肠杆菌伴侣蛋白和人伴侣蛋白的融合蛋白公开(公告)号：US08247206B2
公开(公告)日：2012-08-21
申请号：US13083903
申请日：2011-04-11
CPC分类号： C12N9/90 , C07K2319/35
摘要： The invention discloses the cloning, expression and uses of a chimeric fusion protein with superior chaperone and folding activities compared to the wild type chaperones. This invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule containing nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for an FK506 binding protein (FKBP) or an FK506-binding-protein-like domain (FKBP-like domain). In particular, this invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule containing nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for a human FKBP type peptidyl-prolyl-cis/trans isomerase (PPIase), methods of producing these chimeric fusion proteins and their uses as folding helpers in the production of other proteins and in the process of the production of vaccines or pharmaceuticals, and as folding helpers for performing immunoassays.
摘要翻译： 本发明公开了与野生型伴侣相比，具有优异的伴侣和折叠活性的嵌合融合蛋白的克隆，表达和用途。 本发明涉及由含有编码非人伴侣蛋白多肽结合片段的核苷酸序列的重组DNA分子编码的嵌合融合蛋白和编码FK506结合蛋白（FKBP）或FK506-结合蛋白 - 像域（FKBP样域）。 特别地，本发明涉及由包含编码非人伴侣蛋白多肽结合片段的核苷酸序列的重组DNA分子编码的嵌合融合蛋白和编码人FKBP型肽基 - 脯氨酰 - 顺/反异构酶的核苷酸序列 （PPIase），生产这些嵌合融合蛋白的方法及其在生产其他蛋白质和疫苗或药物生产过程中作为折叠助剂的用途，以及用于进行免疫测定的折叠助剂。
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公开(公告)号：US20090291892A1
公开(公告)日：2009-11-26
申请号：US12436396
申请日：2009-05-06
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译： 公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBP）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞 。 还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。
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