公开(公告)号：US20120028240A1

公开(公告)日：2012-02-02

申请号：US13267718

申请日：2011-10-06

Applicant: Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

Inventor： Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

IPC: C12Q3/00

CPC classification number: C12M33/04 ,  B01L3/021 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2400/0487 ,  C12M33/06 ,  C12M41/48 ,  C12M47/04 ,  G01N15/1475 ,  G01N33/56966 ,  G01N2015/1472 ,  G01N2015/1497

Abstract: Animal cell colonies are picked up automatically by an apparatus having a picking head with a plurality of hollow pins and an integrated imager for capturing an image of adherent or non-adherent animal cell colonies held in liquid or semi-solid medium. Image processing identifies the locations of the animal cell colonies to be picked. Picking an animal cell colony is performed by aligning each of the hollow pins in turn with a target animal cell colony location, introducing the hollow pin into the medium, and aspirating the animal cell colony into the hollow pin. In the case of an adherent colony, the distal end of the pin is forced into oscillation to detach the animal cell colony prior to aspiration. The animal cell colony is dispensed into a well plate by increasing pressure in the fluid conduit.

公开(公告)号：US20070156437A1

公开(公告)日：2007-07-05

申请号：US11564284

申请日：2006-11-28

Applicant: ERIC EDWARDS ,  Christopher Mann ,  Jacqueline Parks

Inventor： ERIC EDWARDS ,  Christopher Mann ,  Jacqueline Parks

IPC: G06Q99/00

CPC classification number: G06Q30/02

Abstract: The disclosed subject matter involves marketing a client's product to third parties. The client defines the information to communicate to the third parties. Said information is entered into an automated system which third parties are able to contact at their discretion. When the third party contacts the system, the system relays the advertisement to the third party.

Abstract translation: 所披露的主题涉及将客户的产品营销给第三方。 客户端定义与第三方通信的信息。 所述信息被输入到第三方能够自行决定的自动化系统。 当第三方联系系统时，系统将广告中继给第三方。

公开(公告)号：US20070037220A1

公开(公告)日：2007-02-15

申请号：US11199243

申请日：2005-08-09

Applicant: Julian Burke ,  Christopher Mann

Inventor： Julian Burke ,  Christopher Mann

IPC: G01N33/567

CPC classification number: G01N33/6854 ,  G01N33/6857

Abstract: We disclose a method of detecting a polypeptide of interest produced by a cell or a cell colony, the method comprising: (a) exposing the polypeptide to a class marker capable of identifying a class of polypeptides to which the polypeptide of interest belongs; (b) exposing the polypeptide to a specificity marker capable of identifying a specific binding ability of the polypeptide of interest; and (c) detecting binding of the polypeptide to the class marker or the specificity marker or both.

Abstract translation: 我们公开了一种检测由细胞或细胞集落产生的感兴趣的多肽的方法，所述方法包括：（a）将多肽暴露于能够鉴定感兴趣的多肽的一类多肽的类标记; （b）将多肽暴露于能够鉴定感兴趣的多肽的特异性结合能力的特异性标记; 和（c）检测多肽与类别标记或特异性标记或两者的结合。

公开(公告)号：US06229411B1

公开(公告)日：2001-05-08

申请号：US09117781

申请日：1998-08-06

Applicant: Christopher Mann

Inventor： Christopher Mann

IPC: H01P312

CPC classification number: H01P11/002

Abstract: A waveguide structure is fabricated by patterning active elements on a semiconductor wafer (21). The upper surface of the wafer (21) and the active elements are then coated by a dissolvable positive resist polymer. The polymer is etched using conventional techniques to produce a former for the structure of die waveguide channel and subsequently the polymer former is coated (26) and electroformed (27) using a suitable metallic material. Finally, the polymer former is dissolved leaving an open channel (25) the boundaries of which are defined by the electroformed stucture. The waveguide structure has the advantage that die active elements are integral with the waveguide structure and lie in a common fabrication plane which means that if the depth of the waveguide varies the active elements remain in the same plane. The waveguide structure is particularly suited for use at terahertz frequencies.

Abstract translation: 通过在半导体晶片（21）上构图有源元件来制造波导结构。 然后将晶片（21）的上表面和有源元件用可溶解的正性抗蚀剂聚合物涂覆。 使用常规技术蚀刻聚合物以产生用于管芯波导通道结构的前体，随后使用合适的金属材料涂覆（26）和电铸（27）聚合物成型体。 最后，聚合物成型体溶解，留下开口的通道（25），其边界由电铸结构限定。 波导结构具有的优点是，管芯有源元件与波导结构成一体，并且位于公共制造平面中，这意味着如果波导的深度改变了有源元件保持在同一平面中。 波导结构特别适用于太赫兹频率。

公开(公告)号：US20140352332A1

公开(公告)日：2014-12-04

申请号：US14367740

申请日：2012-12-20

Applicant: Christopher Mann ,  Michael Bennett

Inventor： Christopher Mann ,  Michael Bennett

IPC: F17C7/04

CPC classification number: F17C7/04 ,  F17C13/025 ,  F17C13/026 ,  F17C2203/0391 ,  F17C2221/033 ,  F17C2223/0161 ,  F17C2223/033 ,  F17C2227/0369 ,  F17C2227/0397 ,  F17C2250/0626 ,  F17C2250/0631 ,  F17C2260/037 ,  F17C2265/033 ,  F17C2265/04 ,  F17C2265/07 ,  Y02E50/343

Abstract: Systems and methods for controlling the temperature and pressure of a cryogenic liquid methane storage unit are provided. The disclosed systems and methods generate methane gas from a reservoir of liquid methane stored within the methane storage unit, vent the methane gas through one or more outlet valves connected to the methane storage unit, and generate electric power using the vented methane gas. The generated electric power can then be used to initiating a cooling cycle, which reduces the temperature of said reservoir of liquid methane and reduces the pressure in said methane storage unit. Micro anaerobic digesters and methane storage units may be configured in a networked environment with a central controller that monitors remote units.

Abstract translation: 提供了用于控制低温液体甲烷储存单元的温度和压力的系统和方法。 所公开的系统和方法从存储在甲烷存储单元内的液态甲烷储存器产生甲烷气体，通过连接到甲烷储存单元的一个或多个出口阀排出甲烷气体，并使用排出的甲烷气体产生电力。 所产生的电力然后可用于启动冷却循环，这降低了液体甲烷储存器的温度并降低了所述甲烷储存单元中的压力。 微型厌氧消化器和甲烷存储单元可以在具有监视远程单元的中央控制器的联网环境中配置。

公开(公告)号：US08417011B2

公开(公告)日：2013-04-09

申请号：US12560201

申请日：2009-09-15

Applicant: Kerensa Klottrup ,  Christopher Mann ,  Aaron Figg ,  Julian Francis Burke

Inventor： Kerensa Klottrup ,  Christopher Mann ,  Aaron Figg ,  Julian Francis Burke

IPC: G06K9/00

CPC classification number: G06T7/0012 ,  G06K9/0014 ,  G06T2207/10064 ,  G06T2207/30024

Abstract: The present invention provides in one aspect a method for determining a production level of a polypeptide of interest by a cell or cell colony, comprising obtaining an image of one or more cells or cell colonies; and analyzing the image to detect a signal associated with the polypeptide of interest; wherein a signal level is determined for each cell or cell colony based on signal values from a predefined area surrounding the cell or cell colony, the signal level being indicative of the production level of the polypeptide of interest by the cell or cell colony.

Abstract translation: 本发明在一个方面提供了一种用于确定细胞或细胞集落的感兴趣多肽的产生水平的方法，包括获得一个或多个细胞或细胞集落的图像; 以及分析所述图像以检测与感兴趣的多肽相关的信号; 其中基于来自所述细胞或细胞集落周围的预定义区域的信号值确定每个细胞或细胞集落的信号水平，所述信号水平指示所述细胞或细胞集落所关注的多肽的产生水平。

公开(公告)号：US08293526B2

公开(公告)日：2012-10-23

申请号：US13267695

申请日：2011-10-06

Applicant: Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

Inventor： Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

IPC: C12M1/26 ,  C12M3/00 ,  C12M1/00

CPC classification number: C12M33/04 ,  B01L3/021 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2400/0487 ,  C12M33/06 ,  C12M41/48 ,  C12M47/04 ,  G01N15/1475 ,  G01N33/56966 ,  G01N2015/1472 ,  G01N2015/1497

Abstract: Animal cell colonies are picked up automatically by an apparatus having a picking head with a plurality of hollow pins and an integrated imager for capturing an image of adherent or non-adherent animal cell colonies held in liquid or semi-solid medium. Image processing identifies the locations of the animal cell colonies to be picked. Picking an animal cell colony is performed by aligning each of the hollow pins in turn with a target animal cell colony location, introducing the hollow pin into the medium, and aspirating the animal cell colony into the hollow pin. In the case of an adherent colony, the distal end of the pin is forced into oscillation to detach the animal cell colony prior to aspiration. The animal cell colony is dispensed into a well plate by increasing pressure in the fluid conduit.

公开(公告)号：US20120028239A1

公开(公告)日：2012-02-02

申请号：US13267707

申请日：2011-10-06

Applicant: Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

Inventor： Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

IPC: C12Q3/00

CPC classification number: C12M33/04 ,  B01L3/021 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2400/0487 ,  C12M33/06 ,  C12M41/48 ,  C12M47/04 ,  G01N15/1475 ,  G01N33/56966 ,  G01N2015/1472 ,  G01N2015/1497

Abstract: Animal cell colonies are picked up automatically by an apparatus having a picking head with a plurality of hollow pins and an integrated imager for capturing an image of adherent or non-adherent animal cell colonies held in liquid or semi-solid medium. Image processing identifies the locations of the animal cell colonies to be picked. Picking an animal cell colony is performed by aligning each of the hollow pins in turn with a target animal cell colony location, introducing the hollow pin into the medium, and aspirating the animal cell colony into the hollow pin. In the case of an adherent colony, the distal end of the pin is forced into oscillation to detach the animal cell colony prior to aspiration. The animal cell colony is dispensed into a well plate by increasing pressure in the fluid conduit.

Abstract translation: 动物细胞集落通过具有多个中空针头的拾取头的装置自动拾取，以及用于捕获保持在液体或半固体培养基中的贴壁或非贴壁动物细胞集落的图像的集成成像器。 图像处理识别待挑选的动物细胞集落的位置。 通过将每个中空针依次与目标动物细胞集落位置对准，将中空销引入培养基中，并将动物细胞集落吸入中空销来进行动物细胞集落的采摘。 在附着菌落的情况下，引脚的远端被迫振荡以在抽吸之前分离动物细胞集落。 通过增加流体导管中的压力将动物细胞集落分配到孔板中。

公开(公告)号：US07776584B2

公开(公告)日：2010-08-17

申请号：US10631845

申请日：2003-08-01

Applicant: Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

Inventor： Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

IPC: C12M1/26 ,  C12M3/00 ,  C12M1/36 ,  C12M1/38

CPC classification number: C12M33/04 ,  B01L3/021 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2400/0487 ,  C12M33/06 ,  C12M41/48 ,  C12M47/04 ,  G01N15/1475 ,  G01N33/56966 ,  G01N2015/1472 ,  G01N2015/1497

Abstract: An apparatus and method for automated picking of animal cell colonies. A picking head comprising a plurality of hollow pins is provided. The apparatus has an integrated imager for capturing an image of adherent or non-adherent animal cell colonies held in liquid or semi-solid medium. Image processing identifies the locations of the animal cell colonies to be picked. Picking an animal cell colony is performed by aligning each of the hollow pins in turn with a target animal cell colony location, introducing the hollow pin into the medium, and aspirating the animal cell colony into the hollow pin. In the case of an adherent colony, the distal end of the pin is forced into oscillation to detach the animal cell colony prior to aspiration. The animal cell colony is dispensed into a well plate by increasing pressure in the fluid conduit.

Abstract translation: 用于自动采集动物细胞集落的装置和方法。 提供包括多个中空销的拾取头。 该装置具有用于捕获保持在液体或半固体培养基中的贴壁或非粘附动物细胞集落的图像的集成成像器。 图像处理识别待挑选的动物细胞集落的位置。 通过将每个中空针依次与目标动物细胞集落位置对准，将中空销引入培养基中，并将动物细胞集落吸入中空销来进行动物细胞集落的采摘。 在附着菌落的情况下，引脚的远端被迫振荡以在抽吸之前分离动物细胞集落。 通过增加流体导管中的压力将动物细胞集落分配到孔板中。

公开(公告)号：US07579596B2

公开(公告)日：2009-08-25

申请号：US10571901

申请日：2004-09-15

Applicant: Jonathan James ,  Christopher Mann

Inventor： Jonathan James ,  Christopher Mann

IPC: G01J5/02

CPC classification number: G01V8/005

Abstract: The terahertz camera has a fixed objective lens (2) and a plurality of detectors (3) positioned at the focal plane of the objective lens (2). Each of the detectors (3) is mounted on a movable support (10) so that the antenna is capable of movement across the focal plane of the objective lens (2) and is provided with a flexible waveguide (4) for connecting the output of the detector (3) with signal processing means. Each detector (3) is also provided with a retroreflector (6) which is reflective at frequencies other than terahertz frequencies. During use of the camera, the retroreflector (6) of each detector (3) is illuminated at non-terahertz frequencies so that the spatial position of each detector (3) and hence the spatial source of signals generated by the detector can be accurately identified. The terahertz camera is particularly suited for use in security installations and in chemical and food processing industries.

Abstract translation: 太赫兹相机具有固定物镜（2）和位于物镜（2）的焦平面处的多个检测器（3）。 每个检测器（3）安装在可移动支撑件（10）上，使得天线能够移动穿过物镜（2）的焦平面，并且设置有柔性波导（4），用于将 具有信号处理装置的检测器（3）。 每个检测器（3）还设置有后向反射器（6），反射器以不同于太赫兹频率的频率反射。 在使用相机期间，每个检测器（3）的后向反射器（6）以非太赫兹频率被照射，使得每个检测器（3）的空间位置以及因此可以准确地识别检测器产生的信号的空间信号源 。 太赫兹相机特别适用于安全装置和化学和食品加工业。