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    Animal cell colony picking apparatus and method
    1.
    发明授权
    Animal cell colony picking apparatus and method 有权
    动物细胞集落采摘装置及方法

    公开(公告)号：US08293520B2

    公开(公告)日：2012-10-23

    申请号：US13267707

    申请日：2011-10-06

    Applicant: Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

    Inventor： Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

    IPC: C12M1/26 ,  C12M3/00 ,  C12M1/34

    CPC classification number: C12M33/04 ,  B01L3/021 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2400/0487 ,  C12M33/06 ,  C12M41/48 ,  C12M47/04 ,  G01N15/1475 ,  G01N33/56966 ,  G01N2015/1472 ,  G01N2015/1497

    Abstract: Animal cell colonies are picked up automatically by an apparatus having a picking head with a plurality of hollow pins and an integrated imager for capturing an image of adherent or non-adherent animal cell colonies held in liquid or semi-solid medium. Image processing identifies the locations of the animal cell colonies to be picked. Picking an animal cell colony is performed by aligning each of the hollow pins in turn with a target animal cell colony location, introducing the hollow pin into the medium, and aspirating the animal cell colony into the hollow pin. In the case of an adherent colony, the distal end of the pin is forced into oscillation to detach the animal cell colony prior to aspiration. The animal cell colony is dispensed into a well plate by increasing pressure in the fluid conduit.

    Abstract translation: 动物细胞集落通过具有多个中空针头的拾取头的装置自动拾取，以及用于捕获保持在液体或半固体培养基中的贴壁或非贴壁动物细胞集落的图像的集成成像器。 图像处理识别待挑选的动物细胞集落的位置。 通过将每个中空针依次与目标动物细胞集落位置对准，将中空销引入培养基中，并将动物细胞集落吸入中空销来进行动物细胞集落的采摘。 在附着菌落的情况下，引脚的远端被迫振荡以在抽吸之前分离动物细胞集落。 通过增加流体导管中的压力将动物细胞集落分配到孔板中。

    Animal cell colony picking apparatus and method
    4.
    发明授权
    Animal cell colony picking apparatus and method 有权
    动物细胞集落采摘装置及方法

    公开(公告)号：US08034625B2

    公开(公告)日：2011-10-11

    申请号：US11401966

    申请日：2006-04-12

    Applicant: Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

    Inventor： Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

    IPC: G01N21/00

    CPC classification number: C12M33/04 ,  B01L3/021 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2400/0487 ,  C12M33/06 ,  C12M41/48 ,  C12M47/04 ,  G01N15/1475 ,  G01N33/56966 ,  G01N2015/1472 ,  G01N2015/1497

    Abstract: An apparatus and method for automated picking of animal cell colonies is described. A picking head comprising a plurality of hollow pins is provided. The apparatus has an integrated imager for capturing an image of adherent or non-adherent animal cell colonies held in liquid or semi-solid medium. Image processing identifies the locations of the animal cell colonies to be picked. Picking an animal cell colony is performed by aligning each of the hollow pins in turn with a target animal cell colony location, introducing the hollow pin into the medium, and aspirating the animal cell colony into the hollow pin. In the case of an adherent colony, the distal end of the pin is forced into oscillation to detach the animal cell colony prior to aspiration. The animal cell colony is dispensed into a well plate by increasing pressure in the fluid conduit.

    Abstract translation: 描述了用于动物细胞集落的自动采集的装置和方法。 提供包括多个中空销的拾取头。 该装置具有用于捕获保持在液体或半固体培养基中的贴壁或非粘附动物细胞集落的图像的集成成像器。 图像处理识别待挑选的动物细胞集落的位置。 通过将每个中空针依次与目标动物细胞集落位置对准，将中空销引入培养基中，并将动物细胞集落吸入中空销来进行动物细胞集落的采摘。 在附着菌落的情况下，引脚的远端被迫振荡以在抽吸之前分离动物细胞集落。 通过增加流体导管中的压力将动物细胞集落分配到孔板中。

    AUTOMATED METHOD, SYSTEM, AND PROGRAM FOR GENERATION OF AN AUDIO SURVEY
    6.
    发明申请
    AUTOMATED METHOD, SYSTEM, AND PROGRAM FOR GENERATION OF AN AUDIO SURVEY 审中-公开
    用于生成音频调查的自动方法，系统和程序

    公开(公告)号：US20070219849A1

    公开(公告)日：2007-09-20

    申请号：US11564288

    申请日：2006-11-28

    Applicant: Eric Edwards ,  Christopher Mann ,  Jacqueline Parks

    Inventor： Eric Edwards ,  Christopher Mann ,  Jacqueline Parks

    IPC: G07G1/00

    CPC classification number: G06Q30/02

    Abstract: The disclosed subject matter involves method and system for creating a survey for clients to be administered to third parties. The client provides information as to the survey parameters. The survey is placed in an automated audio format and administered to third parties. Options available include the use of a knowledge base that allows a structure to aid in survey generation. Vocal personas are used that make the surveys easier to use and seem more creditable.

    Abstract translation: 所披露的主题涉及为向第三方管理的客户端创建调查的方法和系统。 客户端提供有关测量参数的信息。 调查采用自动化音频格式，并向第三方管理。 可用的选项包括使用一个知识库，允许一个结构来帮助调查生成。 使用声乐角色，使调查更容易使用，似乎更加值得信赖。

    THREE-DIMENSIONAL DISPLAY USING OPTICAL FIBERS OF DIFFERENT LENGTHS
    8.
    发明申请
    THREE-DIMENSIONAL DISPLAY USING OPTICAL FIBERS OF DIFFERENT LENGTHS 失效
    使用不同长度的光纤的三维显示

    公开(公告)号：US20050122479A1

    公开(公告)日：2005-06-09

    申请号：US10731848

    申请日：2003-12-06

    Applicant: Christopher Mann

    Inventor： Christopher Mann

    IPC: G03B21/26 ,  G06T15/00 ,  H04N13/00 ,  H05B35/00

    CPC classification number: H04N13/395

    Abstract: A system for viewing 3-D images that comprises an image source (50), a computer (105) and a television screen (110). The television screen is divided into a plurality of sections (115-130), with each section displaying an image representative of a certain depth in the image, as provided by the computer. Image display elements comprising optical fiber bundles (200-215) or lamps, (e.g. 520) and leads (e.g. 540) convey individual depth images from the television screen to a plastic block (225) in which the fiber bundles terminate or the lamps are positioned. The individual fiber bundles terminate at predetermined depths within the plastic block, with those conveying the nearest part of an image terminating at the front of the block, as viewed by an observer (220). The fiber bundle or lamp conveying the greatest depth in the image terminates near the rear portion of the block, as seen by an observer. Fiber bundles or lamps conveying images of intermediate distances terminate at intermediate distances within the block. Fiber diameters and lamp sizes graduate from large to small as depth in the image increases. A true 3-D image is thus provided.

    Abstract translation: 一种用于观看包括图像源（50），计算机（105）和电视屏幕（110）的三维图像的系统。 电视屏幕被分成多个部分（115-130），每个部分显示代表图像中一定深度的图像，如计算机所提供的。 包括光纤束（200-215）或灯（例如520）和引线（例如540）的图像显示元件将各个深度图像从电视屏幕传送到塑料块（225），其中纤维束终止或灯是 定位。 单个纤维束在塑料块内的预定深度处终止，如观察者（220）所观察到的那样，那些传送最终部分的图像终止在块的前部。 传输图像中最大深度的光纤束或灯在观察者看到的情况下，在块的后部附近终止。 传输中间距离图像的光纤束或灯在块内的中间距离处终止。 随着图像深度的增加，光纤直径和灯泡尺寸从大到小逐渐减小。 因此提供真实的3-D图像。

    Animal cell colony picking apparatus and method
    10.
    发明授权
    Animal cell colony picking apparatus and method 有权
    动物细胞集落采摘装置及方法

    公开(公告)号：US08034612B2

    公开(公告)日：2011-10-11

    申请号：US12318612

    申请日：2009-01-02

    Applicant: Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

    Inventor： Stephen Richmond ,  Christopher Mann ,  Andrew Board ,  Yonggang Jiang

    IPC: C12M1/26 ,  C12M3/00 ,  C12M1/34

    CPC classification number: C12M33/04 ,  B01L3/021 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2400/0487 ,  C12M33/06 ,  C12M41/48 ,  C12M47/04 ,  G01N15/1475 ,  G01N33/56966 ,  G01N2015/1472 ,  G01N2015/1497

    Abstract: An apparatus and method for automated picking of animal cell colonies. A picking head comprising a plurality of hollow pins is provided. The apparatus has an integrated imager for capturing an image of adherent or non-adherent animal cell colonies held in liquid or semi-solid medium. Image processing identifies the locations of the animal cell colonies to be picked. Picking an animal cell colony is performed by aligning each of the hollow pins in turn with a target animal cell colony location, introducing the hollow pin into the medium, and aspirating the animal cell colony into the hollow pin. In the case of an adherent colony, the distal end of the pin is forced into oscillation to detach the animal cell colony prior to aspiration. The animal cell colony is dispensed into a well plate by increasing pressure in the fluid conduit.

    Abstract translation: 用于自动采集动物细胞集落的装置和方法。 提供包括多个中空销的拾取头。 该装置具有用于捕获保持在液体或半固体培养基中的贴壁或非粘附动物细胞集落的图像的集成成像器。 图像处理识别待挑选的动物细胞集落的位置。 通过将每个中空针依次与目标动物细胞集落位置对准，将空心针引入培养基中，并将动物细胞集落吸入中空针进行动物细胞集落。 在附着菌落的情况下，引脚的远端被迫振荡以在抽吸之前分离动物细胞集落。 通过增加流体导管中的压力将动物细胞集落分配到孔板中。

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