公开(公告)号：US20090155802A1

公开(公告)日：2009-06-18

申请号：US12324491

申请日：2008-11-26

申请人： Keith A. Bauer ,  David Harrow Gelfand

发明人： Keith A. Bauer ,  David Harrow Gelfand

IPC分类号： C12Q1/68 ,  C12N9/12 ,  C12N15/54 ,  C12N15/85 ,  C12N5/10 ,  C12P19/34

CPC分类号： C12N9/1252

摘要： Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

摘要翻译： 公开了相对于相应的未修饰聚合酶具有改进的延伸速率的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。

公开(公告)号：US20090137000A1

公开(公告)日：2009-05-28

申请号：US12170110

申请日：2008-07-09

申请人： Keith A. Bauer ,  David Harrow Gelfand

发明人： Keith A. Bauer ,  David Harrow Gelfand

IPC分类号： C12P21/06 ,  C12N9/10 ,  C07H21/04 ,  C12N5/10 ,  C12P19/34 ,  C12N15/63

CPC分类号： C12N9/1276

摘要： Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

公开(公告)号：US4870017A

公开(公告)日：1989-09-26

申请号：US778414

申请日：1985-09-20

申请人： Arie Ben-Bassat ,  Keith A. Bauer ,  Shing Chang ,  Sheng-Yung Chang

发明人： Arie Ben-Bassat ,  Keith A. Bauer ,  Shing Chang ,  Sheng-Yung Chang

IPC分类号： C12N15/09 ,  C07K14/195 ,  C07K14/41 ,  C07K14/415 ,  C07K14/435 ,  C07K14/55 ,  C07K16/00 ,  C12N1/20 ,  C12N9/48 ,  C12N15/57 ,  C12N15/70 ,  C12P21/02 ,  C12P21/06 ,  C12R1/19

CPC分类号： C12P21/02 ,  C07K14/415 ,  C07K14/55 ,  C12N15/70 ,  C12N9/48

摘要： Methods of obtaining N-terminal methionine-free proteins are described. The methods employ a novel enzyme, E. coli methionine aminopeptidase either in vitro or in vivo. For in vivo application, plasmid-borne DNA encoding the peptidase is transformed into a bacterial host which produces the desired protein.

摘要翻译： 描述了获得N-末端不含甲硫氨酸的蛋白质的方法。 该方法在体外或体内采用新型的酶，大肠杆菌甲硫氨酸氨基肽酶。 对于体内应用，将编码肽酶的质粒携带的DNA转化成产生所需蛋白质的细菌宿主。

公开(公告)号：US08026091B2

公开(公告)日：2011-09-27

申请号：US12170110

申请日：2008-07-09

申请人： Keith A. Bauer ,  David Harrow Gelfand

发明人： Keith A. Bauer ,  David Harrow Gelfand

IPC分类号： C12N9/12 ,  C12P19/34

CPC分类号： C12N9/1276

摘要： Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

摘要翻译： 公开了相对于相应的未修饰聚合酶具有改进的延伸速率的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。

公开(公告)号：US4400467A

公开(公告)日：1983-08-23

申请号：US251831

申请日：1981-04-07

申请人： Keith A. Bauer ,  Behzad Khosrovi

发明人： Keith A. Bauer ,  Behzad Khosrovi

IPC分类号： C12P19/06 ,  C12N1/20 ,  C12R1/64

CPC分类号： C12R1/64 ,  C12P19/06 ,  Y10S435/813 ,  Y10S435/91

摘要： This invention involves the production of heteropolysaccharides such as xanthan gum by the continuous fermentation of suitable nutrient media with new degenerative resistant variants of Xanthomanas campestris. Fermentation is conducted in a nitrogen, sulfur or phosphorous limited nutrient medium containing glucose or a minimal medium plus yeast extract and glutamic acid, in a first fermenter to enhance cultural growth. After the limiting growth element (nitrogen, sulfur or phosphorous) has been essentially consumed, the medium is then transferred to a second fermenter and fermentation continued while adding a suitable carbohydrate which the bacteria convert into the heteropolysaccharide product. The process may be modified by carrying out cultural growth and production of the polysaccharide in the same fermenter. The bacteria employed were cultured from a glucose-mineral salts medium fortified with yeast extract or a glucose-defined minimal medium in which ammonium chloride served as the sole assimilable nitrogen source.

摘要翻译： 本发明涉及通过连续发酵合适的营养培养基与野菜的新的退化抗性变体来生产杂多糖如黄原胶。 在含有葡萄糖或基本培养基加酵母提取物和谷氨酸的氮，硫或磷限制营养培养基中，在第一发酵罐中进行发酵以增强文化生长。 在极限生长元素（氮，硫或磷）基本消耗之后，将培养基转移到第二发酵罐中并继续发酵，同时加入细菌转化成杂多糖产物的合适的碳水化合物。 该方法可以通过在同一发酵罐中进行多糖的文化生长和生产来改变。 使用的细菌是由酵母提取物或葡萄糖限定的基本培养基强化的葡萄糖 - 无机盐培养基培养的，其中氯化铵作为唯一可同化的氮源。