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公开(公告)号：US08026091B2
公开(公告)日：2011-09-27
申请号：US12170110
申请日：2008-07-09
CPC分类号： C12N9/1276
摘要： Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
摘要翻译： 公开了相对于相应的未修饰聚合酶具有改进的延伸速率的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。
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2.发明授权Mutant DNA polymerases with improved pyrophosphorolysis activated polymerization (PAP) ability 有权突变型DNA聚合酶具有改善的焦磷酸裂解活化聚合（PAP）能力公开(公告)号：US08071536B2
公开(公告)日：2011-12-06
申请号：US12324491
申请日：2008-11-26
CPC分类号： C12N9/1252
摘要： Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
摘要翻译： 公开了相对于相应的未修饰聚合酶具有改进的延伸速率的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。
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3.发明申请Mutant DNA Polymerases with Improved Pyrophasphorolysis Activated Polymerization (PAP) Ability 有权突变型DNA聚合酶与改良的激活聚合反应（PAP）的能力公开(公告)号：US20090155802A1
公开(公告)日：2009-06-18
申请号：US12324491
申请日：2008-11-26
CPC分类号： C12N9/1252
摘要： Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
摘要翻译： 公开了相对于相应的未修饰聚合酶具有改进的延伸速率的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。
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公开(公告)号：US20090137000A1
公开(公告)日：2009-05-28
申请号：US12170110
申请日：2008-07-09
CPC分类号： C12N9/1276
摘要： Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
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公开(公告)号：US07947817B2
公开(公告)日：2011-05-24
申请号：US10879494
申请日：2004-06-28
CPC分类号： C07H21/04 , C07H19/00 , C07H19/16 , C12Q1/6869
摘要： The invention provides compositions that comprise nucleotides and/or nucleosides having blocking groups at 2′-positions of sugar moieties. Methods of synthesizing these nucleic acids are also provided.
摘要翻译： 本发明提供了包含在糖部分的2'-位具有封闭基团的核苷酸和/或核苷的组合物。 还提供了合成这些核酸的方法。
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公开(公告)号：US5665551A
公开(公告)日：1997-09-09
申请号：US528384
申请日：1995-09-13
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/14 , C12N9/16 , C12N15/00 , C12N15/53 , C12Q1/68 , C12R1/01 , C12R1/19 , C12N15/55
CPC分类号： C12N9/14 , C12Q1/6844
摘要： Purified DNA sequences that encode a thermostable pyrophosphatase are provided. The purified DNA is obtained using a DNA probe consisting of SEQ ID NO: 1. Also provided are methods for producing thermostable pyrophosphatase.
摘要翻译： 提供编码热稳定焦磷酸酶的纯化DNA序列。 使用由SEQ ID NO：1组成的DNA探针获得纯化的DNA。还提供了生产耐热性焦磷酸酶的方法。
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公开(公告)号：US07919249B2
公开(公告)日：2011-04-05
申请号：US12174488
申请日：2008-07-16
申请人： David Harrow Gelfand , Fred Lawrence Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas Myers
发明人： David Harrow Gelfand , Fred Lawrence Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas Myers
CPC分类号： C12Q1/6869 , Y10S435/975 , C12Q2535/101 , C12Q2525/101 , C12Q2525/186
摘要： The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
摘要翻译： 本发明提供扩增引物核酸和测序靶核酸的方法。 所述方法包括使用2'-终止子核苷酸来实现链终止。 除了相关的反应混合物和试剂盒之外，本发明还提供计算机和计算机可读介质。
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8.发明授权Thermostable DNA polymerases having reduced discrimination against ribo-NTPs 失效热稳定的DNA聚合酶减少了对ribo-NTPs的歧视
公开(公告)号：US5939292A
公开(公告)日：1999-08-17
申请号：US906484
申请日：1997-08-05
IPC分类号： C12N15/09 , C12N1/21 , C12N9/12 , C12N15/10 , C12N15/54 , C12P21/02 , C12Q1/68 , C12R1/19 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6848 , C12N9/1252 , C12Q1/6869
摘要： Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides, such as ribonucleotides, into DNA products, are advantageous in many in vitro synthesis applications. Such enzymes are particularly useful for use in nucleic acid sequencing protocols and provide novel means for DNA sequence analysis. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
摘要翻译： 具有增强的非常规核苷酸（例如核糖核苷酸）掺入DNA产物的效率的修饰的热稳定DNA聚合酶在许多体外合成应用中是有利的。 这些酶特别可用于核酸测序方案，并为DNA序列分析提供新的手段。 编码修饰酶的基因及其生产和使用方法为DNA测序提供了成本和效率优势。
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公开(公告)号：US08163487B2
公开(公告)日：2012-04-24
申请号：US13045427
申请日：2011-03-10
申请人： David Harrow Gelfand , Fred Lawrence Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas W. Myers
发明人： David Harrow Gelfand , Fred Lawrence Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas W. Myers
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , Y10S435/975 , C12Q2535/101 , C12Q2525/101 , C12Q2525/186
摘要： The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
摘要翻译： 本发明提供扩增引物核酸和测序靶核酸的方法。 所述方法包括使用2'-终止子核苷酸来实现链终止。 除了相关的反应混合物和试剂盒之外，本发明还提供计算机和计算机可读介质。
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公开(公告)号：US06228628B1
公开(公告)日：2001-05-08
申请号：US09105697
申请日：1998-06-26
IPC分类号： C12N912
CPC分类号： C12Q1/6869 , C07K2319/00 , C12N9/1252
摘要： Mutant, chimeric thermostable DNA polymerases are provided, along with purified DNA sequences that encode the enzymes. Also provided are methods for producing and using the enzymes.
摘要翻译： 提供突变型嵌合热稳定DNA聚合酶，以及编码酶的纯化DNA序列。 还提供了用于生产和使用酶的方法。
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