公开(公告)号：US20200255876A1

公开(公告)日：2020-08-13

申请号：US16859618

申请日：2020-04-27

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM

IPC: C12P19/14 ,  C12P19/02

Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and e) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

公开(公告)号：US20200048671A1

公开(公告)日：2020-02-13

申请号：US16557256

申请日：2019-08-30

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM

IPC: C12P19/14 ,  C12P7/14 ,  C12N9/30 ,  C12P7/10 ,  C12P19/02

Abstract: A method of treating a patient who has melanoma includes administering to said patient a composition containing a population of activated T cells that selectively recognize cells in the patient that aberrantly express a peptide. A pharmaceutical composition contains activated T cells that selectively recognize cells in a patient that aberrantly express a peptide, and a pharmaceutically acceptable carrier, in which the T cells bind to the peptide in a complex with an MHC class I molecule, and the composition is for treating the patient who has melanoma. A method of treating a patient who has melanoma includes administering to said patient a composition comprising a peptide in the form of a pharmaceutically acceptable salt, thereby inducing a T-cell response to the melanoma.

公开(公告)号：US20180237804A1

公开(公告)日：2018-08-23

申请号：US15956841

申请日：2018-04-19

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

IPC: C12P7/10 ,  C12P7/54 ,  C12P7/16 ,  C12P7/18 ,  C12P35/00 ,  C12P19/14 ,  C12P19/02 ,  C12P17/10 ,  C12P7/56 ,  C12P5/02 ,  C12P7/48 ,  C12P7/46 ,  C12P7/42 ,  C12P7/40 ,  C12P7/20

CPC classification number: C12P7/10 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/343

Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

公开(公告)号：US20180208949A1

公开(公告)日：2018-07-26

申请号：US15934636

申请日：2018-03-23

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT ,  Aida HISENI

IPC: C12P7/10 ,  C12P19/14 ,  C12P17/10 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P35/00 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P19/02 ,  C12P7/20

Abstract: The invention relates to a process for the preparation of a sugar product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and d) optionally recovery of a sugar product; wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

公开(公告)号：US20210115482A1

公开(公告)日：2021-04-22

申请号：US17115429

申请日：2020-12-08

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

IPC: C12P19/02 ,  C13K1/02 ,  D21C3/26 ,  C13K13/00 ,  C12P19/14

Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

公开(公告)号：US20200224223A1

公开(公告)日：2020-07-16

申请号：US16837637

申请日：2020-04-01

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT ,  Aida HISENI

IPC: C12P7/10 ,  C12P19/14 ,  C12P19/02 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P35/00

Abstract: The invention relates to a process for the preparation of a sugar product from lignocellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and d) optionally recovery of a sugar product; wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

公开(公告)号：US20200048669A1

公开(公告)日：2020-02-13

申请号：US16528191

申请日：2019-07-31

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

IPC: C12P19/02 ,  C13K13/00 ,  D21C3/26 ,  C13K1/02 ,  C12P19/14

Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

公开(公告)号：US20190062794A1

公开(公告)日：2019-02-28

申请号：US16173805

申请日：2018-10-29

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef Berkhout

IPC: C12P19/02 ,  D21C3/26 ,  C13K13/00 ,  C12P19/14 ,  C13K1/02

CPC classification number: C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C13K1/02 ,  C13K13/00 ,  C13K13/002 ,  D21C3/26 ,  Y02E50/16

Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

公开(公告)号：US20170044584A1

公开(公告)日：2017-02-16

申请号：US15307046

申请日：2015-04-29

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C13K1/02 ,  C13K13/00 ,  C13K13/002 ,  D21C3/26 ,  Y02E50/16

Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

Abstract translation: 本发明涉及从木质纤维素材料制备发酵产物的方法，包括以下步骤：a）任选地，木质纤维素材料的预处理，b）任选地，任选地，洗涤任选预处理的木质纤维素材料，c）酶水解 任选地使用包含至少两种纤维素酶的酶组合物洗涤和/或任选地预处理的木质纤维素材料，并且由此所述酶组合物至少包含LPMO，并任选地纯化水解的木质纤维素材料，d）水解的木质纤维素材料的发酵以产生发酵产物， 和e）任选地，回收发酵产物，其中在预处理之后和在酶水解之前和/或期间，向木质纤维素材料中加入电子给体和氧。

公开(公告)号：US20150307903A1

公开(公告)日：2015-10-29

申请号：US14440662

申请日：2013-11-07

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

IPC: C12P7/10 ,  C12P19/14 ,  C12P7/56 ,  C12P7/42 ,  C12P7/40 ,  C12P7/54 ,  C12P7/46 ,  C12P7/48 ,  C12P7/18 ,  C12P5/02 ,  C12P7/20 ,  C12P7/16 ,  C12P17/10 ,  C12P35/00 ,  C12P19/02

CPC classification number: C12P7/10 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/343

Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

Abstract translation: 本发明涉及一种从木质纤维素材料制备发酵产物的方法，包括以下步骤：a）任选地预处理木素纤维素材料; b）任选地洗涤任选预处理的木质纤维素材料; c）使用包含至少两种纤维素酶的酶组合物对任选洗涤和/或任选预处理的木质纤维素材料进行酶水解，由此所述酶组合物至少包含GH61; d）使用小于7.5mg酶组合物/ g葡聚糖（以干物质和酶为蛋白质）或小于3.0mg酶组合物/ g原料（以干物质和酶为蛋白质）; 和e）水解的木质纤维素材料的发酵以产生发酵产物; 和f）任选地回收发酵产物; 其中在酶水解之前和/或期间，向木质纤维素材料中加入氧气。