公开(公告)号：US20210087256A1

公开(公告)日：2021-03-25

申请号：US16984655

申请日：2020-08-04

Applicant: Dyax Corp.

Inventor： Robert Charles Ladner ,  Edward H. Cohen ,  Horacio G. Nastri ,  Kristin L. Rookey ,  Rene Hoet ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC: C07K16/00 ,  C12N15/10 ,  C12N15/66 ,  C40B40/02

Abstract: Methods useful in constructing libraries that collectively display and/or express members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens.

公开(公告)号：US10718066B2

公开(公告)日：2020-07-21

申请号：US15836230

申请日：2017-12-08

Applicant: Dyax Corp.

Inventor： Robert Charles Ladner

IPC: C40B40/08 ,  C07K16/00 ,  C07K16/12 ,  C07K16/28 ,  C40B50/06

Abstract: Provided are compositions and methods for preparing and identifying antibodies having CDR3s that vary in sequence and in length from very short to very long which in certain embodiments may bind to a carbohydrate moiety or the active site of an enzyme. Libraries coding for antibodies with the CDR3s are also provided. The libraries can be provided by modifying a pre-existing nucleic acid library.

公开(公告)号：US09803190B2

公开(公告)日：2017-10-31

申请号：US13754261

申请日：2013-01-30

Applicant: Dyax Corp.

Inventor： Robert Charles Ladner

IPC: C40B40/10 ,  C12N15/10 ,  C07K16/00 ,  C40B40/02 ,  C40B40/08

CPC classification number: C12N15/1037 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/565 ,  C40B40/02 ,  C40B40/08 ,  C40B40/10

Abstract: Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies.

公开(公告)号：US09757437B2

公开(公告)日：2017-09-12

申请号：US14271105

申请日：2014-05-06

Applicant: DYAX CORP.

Inventor： Henry Blair ,  Thomas Beck ,  Robert Charles Ladner

IPC: A61K38/55 ,  A61K38/57 ,  A61K31/195 ,  A61K45/06

CPC classification number: A61K38/55 ,  A61K31/195 ,  A61K38/57 ,  A61K45/06 ,  A61K2300/00

Abstract: Methods, kits and compositions are described that include a non-naturally occurring kallikrein inhibitor and an anti-thrombolytic agent, e.g., an anti-fibrinolytic agent, for preventing or reducing blood loss and/or ischemia, e.g., ischemia associated with perioperative blood loss and cerebral ischemia, the onset of systemic inflammatory response, and/or reperfusion injury, e.g., reperfusion injury associated with cerebral ischemia or a focal brain ischemia, e.g., in patients subjected to invasive surgical procedures, especially procedures requiring cardiopulmonary bypass.

公开(公告)号：US09562902B2

公开(公告)日：2017-02-07

申请号：US14498340

申请日：2014-09-26

Applicant: Dyax Corp.

Inventor： Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Jurgen Mullberg ,  Robert Charles Ladner

IPC: C40B30/04 ,  G01N33/569 ,  C07K16/00 ,  C12N15/85 ,  C12N15/10 ,  C12N15/62

CPC classification number: C12N15/1082 ,  C07K16/00 ,  C07K2317/56 ,  C12N15/1058 ,  C12N15/625 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/002 ,  C12N2830/50 ,  C12N2830/55 ,  C12N2830/85 ,  C12N2840/203 ,  C40B30/04 ,  G01N33/56983

Abstract: Disclosed is a method that includes: (i) providing a plurality of initial nucleic acid cassettes that include: a) a first coding region encoding a first immunoglobulin variable domain, b) a second coding region encoding a second immunoglobulin variable domain, and c) a ribosomal binding site disposed between the first and second coding regions for translation of the second polypeptide in a first expression system, wherein the first and second coding regions are in the same translational orientation; (ii) modifying each nucleic acid cassette of the plurality in a single reaction mixture so that it is functional in a second expression system, wherein the first and second region remain physically attached during the modifying; (iii) introducing each modified nucleic acid cassette into a mammalian cell to produce a mixture of transfected cells; and (iv) expressing each modified nucleic acid cassette in the transfected cells.

Abstract translation: 公开了一种方法，其包括：（i）提供多个初始核酸盒，其包括：a）编码第一免疫球蛋白可变结构域的第一编码区，b）编码第二免疫球蛋白可变结构域的第二编码区，和c） 布置在第一和第二编码区之间用于在第一表达系统中翻译第二多肽的核糖体结合位点，其中第一和第二编码区具有相同的平移方向; （ii）在单个反应混合物中修饰多个的每个核酸盒，使得其在第二表达系统中起作用，其中第一和第二区域在修饰期间保持物理附着; （iii）将每个修饰的核酸盒引入哺乳动物细胞以产生转染细胞的混合物; 和（iv）在转染的细胞中表达每个修饰的核酸盒。

公开(公告)号：US20160355571A1

公开(公告)日：2016-12-08

申请号：US15177199

申请日：2016-06-08

Applicant: Dyax Corp.

Inventor： Robert Charles Ladner

IPC: C07K16/00 ,  C40B40/10 ,  C40B40/02

Abstract: Provided are compositions and methods for preparing and identifying antibodies having CDR3s that vary in sequence and in length from very short to very long which in certain embodiments may bind to a carbohydrate moiety or the active site of an enzyme. Libraries coding for antibodies with the CDR3s are also provided. The libraries can be provided by modifying a pre-existing nucleic acid library.

Abstract translation: 提供了用于制备和鉴定具有CDR3的抗体的组合物和方法，所述抗体具有从非常短到非常长的序列和长度，其在某些实施方案中可能结合于碳水化合物部分或酶的活性位点。 还提供了编码具有CDR3的抗体的文库。 可以通过修饰预先存在的核酸文库来提供文库。

公开(公告)号：US09446155B2

公开(公告)日：2016-09-20

申请号：US14109508

申请日：2013-12-17

Applicant: Dyax Corp. ,  BRACCO SUISSE SA

Inventor： Aaron K. Sato ,  Daniel J. Sexton ,  Daniel T. Dransfield ,  Robert Charles Ladner ,  Christophe Arbogast ,  Philippe Bussat ,  Hong Fan ,  Sudha Khurana ,  Karen E. Linder ,  Edmund R. Marinelli ,  Palaniappa Nanjappan ,  Adrian D. Nunn ,  Radhakrishna K. Pillai ,  Sibylle Pochon ,  Kondareddiar Ramalingam ,  Ajay Shrivastava ,  Bo Song ,  Rolf E. Swenson ,  Mathew A. Von Wronski ,  Feng Yan

IPC: C07K14/00 ,  A61K49/22 ,  A61K47/48 ,  A61K51/08 ,  C07K7/08 ,  C07K14/52 ,  C07K14/71 ,  A61K38/00

CPC classification number: A61K49/221 ,  A61K38/00 ,  A61K47/62 ,  A61K49/223 ,  A61K51/088 ,  C07K7/08 ,  C07K14/001 ,  C07K14/52 ,  C07K14/71 ,  C07K2319/00 ,  Y02A50/411 ,  Y02A50/58

Abstract: The present invention provides polypeptides, peptide dimer, and multimeric complexes comprising at least one binding moiety for KDR or VEGF/KDR complex, which have a variety of uses wherever treating, detecting, isolating or localizing angiogenesis is advantageous. Particularly disclosed are synthetic, isolated polypeptides capable of binding KDR or VEGF/KDR complex with high affinity (e.g., having a KD

Abstract translation: 本发明提供了包含用于KDR或VEGF / KDR复合物的至少一个结合部分的多肽，肽二聚体和多聚体复合物，其具有各种用途，无论何处治疗，检测，分离或定位血管发生都是有利的。 特别公开的是能够以高亲和力（例如具有KD <1μM）结合KDR或VEGF / KDR复合物的合成，分离的多肽，以及包含这些多肽的二聚体和多聚体构建体。

公开(公告)号：US20140135226A1

公开(公告)日：2014-05-15

申请号：US14048526

申请日：2013-10-08

Applicant: Dyax Corp.

Inventor： Robert Charles Ladner ,  Shannon Hogan ,  Kristin L. Rookey

IPC: C12N15/10

CPC classification number: C12N15/1037 ,  B03C1/01 ,  B03C1/288 ,  C40B30/04 ,  G01N33/56983 ,  G01N2500/10

Abstract: Disclosed are methods for identifying desired members from a display libraries, including bacteriophage display libraries. Display library members can be amplified in the presence of a target compound so that cycles of selection can be rapidly completed.

Abstract translation: 公开了用于从显示库识别所需成员的方法，包括噬菌体展示文库。 显示库成员可以在目标化合物存在下扩增，以便可以快速完成选择周期。

公开(公告)号：US20140018261A9

公开(公告)日：2014-01-16

申请号：US13754261

申请日：2013-01-30

Applicant: DYAX CORP.

Inventor： Robert Charles Ladner

IPC: C12N15/10 ,  C07K16/00

CPC classification number: C12N15/1037 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/565 ,  C40B40/02 ,  C40B40/08 ,  C40B40/10

Abstract: Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies.

Abstract translation: 显示，显示和表达或组成抗体肽，多肽或蛋白质不同家族成员的集中的载体文库或遗传包，并且共同显示，显示和表达或包含至少一部分家庭聚焦多样性 。 这些文库具有模拟在天然人抗体中发现的长度和序列多样性。

公开(公告)号：US20190247477A1

公开(公告)日：2019-08-15

申请号：US16281276

申请日：2019-02-21

Applicant: Dyax Corp.

Inventor： Robert Charles Ladner ,  Arthur Charles Ley ,  Shirish Hirani ,  Anthony Williams

IPC: A61K38/57 ,  A61K38/55 ,  C12N9/64 ,  A61K9/00

CPC classification number: A61K38/57 ,  A61K9/0019 ,  A61K38/55 ,  C12N9/6445

Abstract: Methods are described for preventing or reducing ischemia and/or systemic inflammatory response in a patient such as perioperative blood loss and/or systemic inflammatory response in a patient subjected to cardiothoracic surgery, e.g. coronary artery bypass grafting and other surgical procedures, especially when such procedures involve extra-corporeal circulation, such as cardiopulmonary bypass.