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公开(公告)号：US20050182767A1
公开(公告)日：2005-08-18
申请号：US10651733
申请日：2003-08-29
申请人： Daniel Shoemaker , Lee O'Donnell , James Broder , Scott Shoemaker
发明人： Daniel Shoemaker , Lee O'Donnell , James Broder , Scott Shoemaker
IPC分类号： G06F17/30
CPC分类号： G06F21/6218 , G06F3/0484 , G06F17/30312 , G06F17/30345 , G06F17/30575 , H04L63/08 , H04L67/1095 , H04L67/18
摘要： A system for transmission of data between a first device operated by a first user and a second device operated by a second user includes a database that receives a first set of data input by the first user and a second set of data input by the second user. In one embodiment, the second user can access at least a first portion of the first set of data from the database only after the second user authorizes the first user to access at least a first portion of the second set of data from the database. In another embodiment, the first set of data includes an immediate mode of access and/or one or more future modes of access of the first user which correlate to one or more specific time periods during which the future mode of access will become the immediate mode of access. Additionally, the first set of data can include a time-dependent schedule of the future mode of access of the first user. The second device can be a mobile phone having a memory that is automatically updated by the database with data input by the first user. Moreover, the first user can specify a hierarchical privacy level for one or more data fields, and a correlative hierarchical clearance level to the second user. The clearance level is compared with the first privacy level to determine whether the second user has access to any of the data fields.
摘要翻译： 用于在由第一用户操作的第一设备和由第二用户操作的第二设备之间传输数据的系统包括接收由第一用户输入的第一组数据的数据库和由第二用户输入的第二组数据 。 在一个实施例中，只有在第二用户授权第一用户从数据库访问第二组数据的至少第一部分之后，第二用户才可以从数据库访问第一组数据的至少第一部分。 在另一个实施例中，第一组数据包括第一用户的即时接入模式和/或一个或多个未来访问模式，其与未来接入模式将成为立即模式的一个或多个特定时间段相关联 的访问。 此外，第一组数据可以包括第一用户的未来访问模式的时间相关调度。 第二设备可以是具有由数据库自动更新的由第一用户输入的数据的存储器的移动电话。 此外，第一用户可以为一个或多个数据字段指定分级隐私级别，并且向第二用户指定相关的分层间隙级别。 将清除级别与第一隐私级别进行比较以确定第二用户是否可以访问任何数据字段。
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公开(公告)号：US08137912B2
公开(公告)日：2012-03-20
申请号：US11763245
申请日：2007-06-14
CPC分类号： C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N1/30 , G01N33/6893 , G01N2800/385
摘要： The present invention relates to methods for detecting, enriching, and analyzing rare cells that are present in the blood, e.g. fetal cells. The invention further features methods of analyzing rare cell(s) to determine the presence of an abnormality, disease or condition in a subject, e.g. a fetus by analyzing a cellular sample from the subject.
摘要翻译： 本发明涉及用于检测，富集和分析存在于血液中的罕见细胞的方法，例如， 胎儿细胞。 本发明还涉及分析稀有细胞以确定受试者中异常，疾病或病症的存在的方法，例如， 通过分析来自受试者的细胞样品的胎儿。
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公开(公告)号：US20100304978A1
公开(公告)日：2010-12-02
申请号：US12657723
申请日：2010-01-26
CPC分类号： C12Q1/6879 , C12Q1/6841 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16
摘要： The present invention provides methods and compositions for specifically identifying a fetal cell. An initial screening of approximately 400 candidate genes by digital PCR in different fetal and adult tissues identified a subset of 24 gene markers specific for fetal nucleated RBC and trophoblasts. The specific expression of those genes was further evaluated and verified in more defined tissues and isolated cells through quantitative RT-PCR using custom Taqman probes specific for each gene. A subset of fetal cell specific markers (FCM) was tested and validated by RNA fluorescent in situ hybridization (FISH) in blood samples from non-pregnant women, and pre-termination and post-termination pregnant women. Applications of these gene markers include, but are not limited to, distinguishing a fetal cell from a maternal cell for fetal cell identification and genetic diagnosis, identifying circulating fetal cell types in maternal blood, purifying or enriching one or more fetal cells, and enumerating one or more fetal cells during fetal cell enrichment.
摘要翻译： 本发明提供用于特异性鉴定胎儿细胞的方法和组合物。 通过数字PCR在不同的胎儿和成体组织中初步筛选约400个候选基因，鉴定了胎儿有核RBC和滋养细胞特异性的24个基因标记的子集。 通过使用特定于每个基因的定制的Taqman探针通过定量RT-PCR进一步评估和验证了在更确定的组织和分离的细胞中这些基因的特异性表达。 胎儿细胞特异性标志物（FCM）的子集在非孕妇血液样本和终止前和终止后的孕妇中通过RNA荧光原位杂交（FISH）进行测试和验证。 这些基因标记物的应用包括但不限于胎儿细胞区分胎儿细胞鉴定和遗传诊断的胎儿细胞，识别母体血液中的循环胎儿细胞类型，纯化或富集一个或多个胎儿细胞，并列举一个 胎儿细胞富集的胎儿细胞。
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公开(公告)号：US20140030232A1
公开(公告)日：2014-01-30
申请号：US13816723
申请日：2011-08-12
申请人： Daniel Shoemaker , Pratik Multani , Caroline Desponts , David L. Robbins , Paul Grayson , John Mendlein
发明人： Daniel Shoemaker , Pratik Multani , Caroline Desponts , David L. Robbins , Paul Grayson , John Mendlein
IPC分类号： C12N5/0789 , A61K35/28
摘要： The invention provides improved methods for cell therapy. In particular, the invention provides therapeutic compositions of modified hematopoietic stem and/or progenitor cells having improved engraftment and homing properties, and methods of making the therapeutic composition. The invention further provides methods of improving the efficacy of hematopoietic stem and progenitor cell transplantation including transplanting the therapeutic composition to subjects in need of hematopoietic system reconstitution.
摘要翻译： 本发明提供了改进的细胞治疗方法。 特别地，本发明提供了具有改善的植入和归巢性质的修饰的造血干细胞和/或祖细胞的治疗组合物，以及制备治疗组合物的方法。 本发明还提供了改善造血干细胞和祖细胞移植功效的方法，包括将治疗组合物移植到需要造血系统重建的受试者身上。
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15.发明申请DETERMINATION OF FETAL ANEUPLOIDY BY QUANTIFICATION OF GENOMIC DNA FROM MIXED SAMPLES 审中-公开通过从混合样品中定量鉴定基因组DNA来确定天然异构体公开(公告)号：US20130288242A1
公开(公告)日：2013-10-31
申请号：US13738268
申请日：2013-01-10
IPC分类号： C12Q1/68
CPC分类号： G01N1/30 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G16B20/00
摘要： The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample.
摘要翻译： 本发明提供了当与样品中的母体细胞群混合时检测胎儿细胞的存在并测试胎儿异常（即非整倍体）的系统，装置和方法。 此外，本发明提供了确定何时胎儿细胞不足以确定并报告非信息性病例的方法。 本发明涉及量化来自混合样品的基因组DNA的区域。 更具体地，本发明涉及从混合样品中定量DNA多态性。
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公开(公告)号：US20130189689A1
公开(公告)日：2013-07-25
申请号：US13837974
申请日：2013-03-15
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
摘要： The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
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公开(公告)号：US20120207744A1
公开(公告)日：2012-08-16
申请号：US13257291
申请日：2010-03-19
IPC分类号： A61K35/12 , C12N5/074 , C12N5/0735 , A61K38/43 , A61K31/713 , A61K38/02 , A61K39/395 , C12N5/071 , A61K31/7088
CPC分类号： C12N15/111 , C12N5/0696 , C12N15/113 , C12N2310/14 , C12N2310/3515 , C12N2320/30 , C12N2320/32 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2510/00
摘要： The present invention provides compositions and methods of using the compositions to alter the developmental potency of a cell. The present invention provides in vivo and ex vivo cell reprogramming and programming methods suitable for autologous cell therapy and regenerative medicine.
摘要翻译： 本发明提供使用该组合物来改变细胞的发育潜能的组合物和方法。 本发明提供适于自体细胞治疗和再生医学的体内和体外细胞重编程和编程方法。
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18.发明申请COMPOSITIONS COMPRISING CYCLIC AMP ENHANCERS AND/OR EP LIGANDS, AND METHODS OF PREPARING AND USING THE SAME 审中-公开包含环状AMP增强剂和/或EP配体的组合物及其制备和使用方法公开(公告)号：US20120202288A1
公开(公告)日：2012-08-09
申请号：US13257290
申请日：2010-03-18
CPC分类号： A61K31/21 , A61K31/35 , A61K31/557 , A61K38/164 , A61K38/2271 , Y02A50/471 , A61K2300/00
摘要： Provided are improved pharmaceutical compositions that comprise ligands or agonists to prostaglandin EP receptors, and/or cyclic AMP enhancers, and suitable organic solvents that are substantially free of methyl acetate, the compositions being provided for storage and/or use in an endotoxin-free vessel, such as a tube or PE bag. The compositions are suitable for in vitro, ex vivo, and in vivo use, and in particular for ex vivo therapeutic use, such as in hematopoietic stem cell transplants. Also provided are methods of using the compositions in ex vivo therapeutic applications, and methods of preparing the compositions. Kits with instructions on use are also provided.
摘要翻译： 提供了包含前列腺素EP受体的配体或激动剂和/或环AMP增强剂的改进的药物组合物，以及基本上不含乙酸甲酯的合适的有机溶剂，所述组合物用于在不含内毒素的血管中储存和/或使用 ，如管或PE袋。 该组合物适用于体外，离体和体内使用，特别是用于离体治疗用途，例如在造血干细胞移植中。 还提供了在离体治疗应用中使用组合物的方法和制备组合物的方法。 还提供了使用说明的工具包。
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公开(公告)号：US08168389B2
公开(公告)日：2012-05-01
申请号：US12230628
申请日：2008-09-02
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
摘要： The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
摘要翻译： 本发明提供了当与样品中的母体细胞群混合时检测胎儿细胞的存在并测试胎儿异常的系统，装置和方法，例如， 非整倍体 本发明涉及用不同的标记标记所述混合样品中每个细胞中的基因组DNA的区域，其中每个标记对每个细胞是特异性的，并且定量混合样品中每个细胞的基因组DNA的标记区域。 更具体地，本发明涉及从混合样品中的每个细胞定量标记的DNA多态性。
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公开(公告)号：US20050032057A1
公开(公告)日：2005-02-10
申请号：US10485008
申请日：2002-07-17
申请人： Daniel Shoemaker
发明人： Daniel Shoemaker
CPC分类号： C12N15/1096 , C12Q1/6837 , C12Q1/6865 , C12Q2563/107
摘要： In one aspect the present invention provides methods of synthesizing a preparation of nucleic acid molecules, the methods comprising the steps of: (a) utilizing an RNA template to enzymatically synthesize a first DNA molecule that is complementary to at least 50 contiguous bases of the RNA template; (b) utilizing the first DNA molecule as a template to enzymatically synthesize a second DNA molecule, thereby forming a double-stranded DNA molecule wherein the first DNA molecule is hybridized to the second DNA molecule; (c) utilizing the first or second DNA molecule of the double-stranded DNA molecule as a template to enzymatically synthesize a first RNA molecule that is complementary to either the first DNA molecule or to the second DNA molecule; and (d) utilizing the first RNA molecule as a template to enzymatically synthesize a third DNA molecule that is complementary to the first RNA molecule. In another aspect, the present invention provides processed DNA samples prepared by a method of the invention for synthesizing a preparation of nucleic acid molecules. In another aspect, the present invention provides methods for hybridizing a processed DNA sample to a population of immobilized nucleic acid molecules.
摘要翻译： 一方面，本发明提供了合成核酸分子制剂的方法，所述方法包括以下步骤：（a）利用RNA模板来酶促合成与RNA的至少50个连续碱基互补的第一DNA分子 模板; （b）利用第一DNA分子作为模板来酶促合成第二DNA分子，从而形成双链DNA分子，其中第一DNA分子与第二DNA分子杂交; （c）利用双链DNA分子的第一或第二DNA分子作为模板来酶促合成与第一DNA分子或第二DNA分子互补的第一RNA分子; 和（d）利用第一RNA分子作为模板来酶促合成与第一RNA分子互补的第三DNA分子。 另一方面，本发明提供了通过本发明的用于合成核酸分子制剂的方法制备的加工DNA样品。 另一方面，本发明提供了将经处理的DNA样品与固定的核酸分子群杂交的方法。
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