公开(公告)号：US10808240B2

公开(公告)日：2020-10-20

申请号：US16301176

申请日：2017-05-15

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg Stoll ,  Daniel Enderle ,  Mikkel Noerholm ,  Johan Karl Olov Skog

IPC: C12N15/10 ,  C12N15/11 ,  C07H1/06

Abstract: The invention provides novel methods and kits for fully automated high-throughput method for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, novel methods and kits for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles that do not require the use of phenol or chloroform, and for extracting nucleic acids from the extracellular vesicles and/or from the biological samples.

公开(公告)号：US10301681B2

公开(公告)日：2019-05-28

申请号：US14909916

申请日：2014-08-06

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Mikkel Noerholm

IPC: C12Q1/6886 ,  G01N33/48

Abstract: The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from urine samples. The disclosure provides compositions, kits and methods for diagnosing a prostate disorder such as prostate cancer in a male subject.

公开(公告)号：US10174361B2

公开(公告)日：2019-01-08

申请号：US14357475

申请日：2012-11-12

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Leileata Russo

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6883 ,  C12N15/10 ,  C12Q1/6886

Abstract: The present invention is directed to methods of isolating particles, such as nucleic acid-containing particles or microvesicles, from a biological sample and extracting nucleic acids therefrom, wherein the biological sample is cerebrospinal fluid. The present invention further provides methods for aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker associated with a disease or medical condition thereof.

公开(公告)号：US20160177401A1

公开(公告)日：2016-06-23

申请号：US14909916

申请日：2014-08-06

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan Karl Olov Skog ,  Mikkel NOERHOLM

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from urine samples. The disclosure provides compositions, kits and methods for diagnosing a prostate disorder such as prostate cancer in a male subject.

Abstract translation: 本发明一般涉及生物标志物分析领域，特别是从尿液样品中确定基因表达特征。 本公开提供用于诊断男性受试者中前列腺障碍如前列腺癌的组合物，试剂盒和方法。

公开(公告)号：US12018334B2

公开(公告)日：2024-06-25

申请号：US17411606

申请日：2021-08-25

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel Noerholm ,  Susan Belzer ,  Charlotte Romain ,  Johan Karl Olov Skog ,  Leileata M. Russo ,  Wayne Comper

IPC: C12P19/34 ,  C12N7/00 ,  C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12N7/00 ,  C12N2795/18122 ,  C12N2795/18131 ,  C12Q2600/106 ,  C12Q2600/118

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.

公开(公告)号：US11891664B2

公开(公告)日：2024-02-06

申请号：US16613298

申请日：2018-05-16

Applicant: Exosome Diagnostics, Inc. ,  The Brigham and Women's Hospital

Inventor： James Hurley ,  Christine Coticchia ,  Robert Kitchen ,  Vasisht Tadigotla ,  Johan Karl Olov Skog ,  Jamil Azzi

IPC: C12Q1/6883 ,  G01N1/28 ,  G01N33/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158 ,  G01N1/28 ,  G01N33/6893 ,  G01N2800/245 ,  G01N2800/347

Abstract: The invention relates generally to the use of microvesicle biomarkers such as nucleic acids, including nucleic acid signatures, and/or proteins for assessing a kidney transplant rejection in a patient. The invention further relates to assessing, and/or to monitoring kidney transplant rejection in patients who have received a renal transplant.

公开(公告)号：US11268085B2

公开(公告)日：2022-03-08

申请号：US16595278

申请日：2019-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US10465183B2

公开(公告)日：2019-11-05

申请号：US15325021

申请日：2015-07-09

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20170198280A1

公开(公告)日：2017-07-13

申请号：US15325021

申请日：2015-07-09

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.