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22 条记录 (耗时 0.023 秒)

    "> Method for producing 2
    15.
    发明授权
    Method for producing 2"'-O-demethyltylosin 失效
    生产2“' - O-二甲基吗啉的方法

    公开(公告)号：US5063155A

    公开(公告)日：1991-11-05

    申请号：US173860

    申请日：1988-03-28

    申请人： Karen L. Cox ,  Eugene T. Seno ,  Gene M. Wild

    发明人： Karen L. Cox ,  Eugene T. Seno ,  Gene M. Wild

    IPC分类号： C07H17/08 ,  C12N1/21 ,  C12N15/52 ,  C12P19/62 ,  C12R1/465 ,  C12R1/54

    CPC分类号： C12N15/52 ,  C12P19/62 ,  Y10S435/886

    摘要： The present invention discloses a method for producing a novel antibiotic, 2"'-O-demethyltylosin. The novel antibiotic, utilizing recombinant DNA technology, can be produced from a transformed mutant of a tylosin producing microorganism. By transforming for example Streptomyces fradiae GS 16 with plasmid pHJL284, the transformant can produce 2"'-O-demethyltylosin. Streytomyces fradiae GS16 is a tylosin producing species that contain a mutation in the tylE gene found in the tylosin biosynthetic pathway. The tylE gene codes for demethylmacrocin 2"'-O-methyltransferase enzyme (DMOMT), the enzyme that methylates the 2-hydroxyl position of the 6-deoxyallose moiety. Plasmid pHJL284 contains the cloned tylF gene which codes for the macrocin 3"'-O-methyltransferase enzxyme (MOMT), the enzyme that methylates the 3"'-hydroxyl position, but it does not contain the tylE gene. Transformation of S. fradiae GS16 with plasmid pHJL284 results in increased copies of the tylF gene in the absence of the tylE gene, therefore allowing an increased rate of methylation of the 3"'-hydroxyl position in the absence of the methyl group at the 2"'-hydroxyl position. The resulting transformant, therefore, can produce 2"'-O-demethyltylosin in appreciable quantities. The present invention is not limited to S. fradiae.For example, two other tylosin producing species such as Streptomyces rimosus and Streptomyces hygroscopicus, may also be used for purposes of the invention disclosed. 2"'-O-Demethyltylosin is useful as an antibiotic with a microbial inhibitory activity similar to tylosin.

    Method for producing 20-deoxotylosin
    16.
    发明授权
    Method for producing 20-deoxotylosin 失效
    生产20-脱氧吗啉的方法

    公开(公告)号：US5168052A

    公开(公告)日：1992-12-01

    申请号：US497158

    申请日：1990-03-20

    申请人： Karen L. Cox ,  Eugene T. Seno

    发明人： Karen L. Cox ,  Eugene T. Seno

    IPC分类号： C12N15/52 ,  C12P19/62

    CPC分类号： C12P19/62 ,  C12N15/52 ,  Y10S435/886

    摘要： The present invention, utilizing recombinant DNA technology, provides a novel method for obtaining 20-deoxotylosin from a tylosin producing microorganism. 20-deoxotylosin is useful as an antibiotic with a microbial inhibitory activity similar to tylosin.

    摘要翻译： 本发明利用重组DNA技术提供了从产生泰乐菌素的微生物获得20-脱氧新霉素的新方法。 20-脱氧噻洛欣可用作具有类似于泰乐菌素的微生物抑制活性的抗生素。

    Tylosin biosynthetic genes tylA, tylB and tylI
    17.
    发明授权
    Tylosin biosynthetic genes tylA, tylB and tylI 失效
    泰乐菌素生物合成基因tylA，tylB和tylI

    公开(公告)号：US5149638A

    公开(公告)日：1992-09-22

    申请号：US741204

    申请日：1991-07-30

    申请人： Robert J. Beckmann ,  Karen L. Cox ,  Eugene T. Seno

    发明人： Robert J. Beckmann ,  Karen L. Cox ,  Eugene T. Seno

    IPC分类号： C12N1/21 ,  C12N15/52 ,  C12N15/76 ,  C12P19/62

    CPC分类号： C12N15/52 ,  C12N15/76 ,  C12P19/62

    摘要： Provided are gene sequences encoding tylosin biosynthetic gene products. In particular, recombinant DNA vectors comprising DNA sequences encoding the tylA, tylB, tylI and tylG activities of Streptomyces fradiae are provided. Also provided are host cells transformed with the noted vectors and a method for increasing the tylosin-producing ability of a tylosin-producing organism.

    摘要翻译： 提供编码泰乐菌素生物合成基因产物的基因序列。 特别地，提供了包含编码Streptomyces fradiae的tylA，tylB，tylI和tylG活性的DNA序列的重组DNA载体。 还提供了用所述载体转化的宿主细胞和用于增加产生泰乐菌素生物体的泰乐菌素生产能力的方法。

    Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms
    20.
    发明授权
    Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms 失效
    增加产生抗生素的微生物的抗生素产生能力的方法

    公开(公告)号：US5672497A

    公开(公告)日：1997-09-30

    申请号：US575843

    申请日：1995-12-21

    申请人： Karen L. Cox ,  Scott E. Fishman ,  Charles L. Hershberger ,  Eugene T. Seno

    发明人： Karen L. Cox ,  Scott E. Fishman ,  Charles L. Hershberger ,  Eugene T. Seno

    IPC分类号： C12P19/62 ,  C12N15/00 ,  C07H21/04 ,  C12N15/09

    CPC分类号： C12P19/62

    摘要： A method for increasing the antibiotic-producing ability of an antibiotic-producing microbial host cell is disclosed. The method involves transforming an antibiotic-producing microorganism with a recombinant DNA cloning vector that codes for the expression of an antibiotic biosynthetic enzyme or other gene product. The gene preferably codes for a product that is rate-limiting in the antibiotic biosynthetic pathway in Streptomyces. Plasmids that are useful for increasing tylosin production in accordance with the present method include plasmids pHJL280, pHJL284, pHJL309, pHJL311, and pHJL315. The invention further comprises microorganisms transformed with plasmids pHJL280, pHJL284, pHJL309, pHJL311, and pHJL315 and also other microorganisms and vectors used in accordance with the present method.

    摘要翻译： 公开了提高产生抗生素的微生物宿主细胞的抗生素产生能力的方法。 该方法包括用编码抗生素生物合成酶或其他基因产物表达的重组DNA克隆载体转化产生抗生素的微生物。 该基因优选编码在链霉菌中的抗生素生物合成途径中限速的产物。 根据本方法可用于增加泰乐菌素生产的质粒包括质粒pHJL280，pHJL284，pHJL309，pHJL311和pHJL315。 本发明还包括用质粒pHJL280，pHJL284，pHJL309，pHJL311和pHJL315转化的微生物以及根据本方法使用的其它微生物和载体。