公开(公告)号：US20190023735A1

公开(公告)日：2019-01-24

申请号：US16115756

申请日：2018-08-29

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Gustav Rodrigo ,  Bjorn Noren ,  Virendra Kumbhar

IPC: C07K1/16 ,  B01J20/32 ,  C07K1/36 ,  B01J20/28 ,  B01J20/26 ,  B01D15/38 ,  G01N33/544 ,  B01J20/289 ,  B01J41/20 ,  B01D15/36 ,  B01D15/32

Abstract: The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R1-L1-N(R3)-L2-R, immobilized on a support, wherein R1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L1 is either a methylene group or a covalent bond; R2 is a five-or six-membered, substituted or non-substituted ring structure; L2 is either a methylene group or a covalent bond; R3 is a methyl group; and wherein if R1 is a hydroxyethyl group and L1 is a covalent bond, R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

公开(公告)号：US10751645B2

公开(公告)日：2020-08-25

申请号：US15882998

申请日：2018-01-29

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J Johansson ,  Jean-Luc Maloisel

IPC: B01D15/38 ,  B01D15/32 ,  B01D15/36 ,  B01J20/32 ,  B01J20/286 ,  B01J41/20 ,  C07K1/36 ,  C07K16/06 ,  C07K1/16 ,  C07K16/00

Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

公开(公告)号：US20190381480A1

公开(公告)日：2019-12-19

申请号：US16486653

申请日：2018-02-15

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Ronnie Palmgren ,  Jean-Luc Maloisel ,  Gustav Rodrigo ,  Tomas Bjorkman

IPC: B01J20/286 ,  B01D15/38 ,  B01J20/28 ,  B01J20/32 ,  B01D15/18 ,  C07K1/22

Abstract: A separation matrix comprising porous particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is above 5 mg/ml, the volume-weighted median diameter of said porous particles is at least 10 and below 30 μm and the said porous particles have a gel phase distribution coefficient, expressed as KD for dextran of molecular weight 110 kDa, of 0.5-0.9.

公开(公告)号：US20180223003A1

公开(公告)日：2018-08-09

申请号：US15937428

申请日：2018-03-27

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

IPC: C08B37/00 ,  C07C323/60 ,  C07C237/10 ,  B01J20/32 ,  B01J20/281 ,  B01J20/289

CPC classification number: C08B37/0039 ,  B01J20/281 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3265 ,  C07C237/10 ,  C07C323/60

Abstract: The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.

公开(公告)号：US20160298065A1

公开(公告)日：2016-10-13

申请号：US15037539

申请日：2014-11-26

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Tobias Soderman ,  Ola Lind

IPC: C12H1/056 ,  B01J20/32 ,  B01J20/28 ,  C08F251/00

CPC classification number: C12H1/0424 ,  B01D15/00 ,  B01J20/28004 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/327 ,  B01J20/3278 ,  B01J20/3293 ,  B01J2220/58 ,  C08F251/00

Abstract: The invention discloses a separation matrix with a porous solid support and a plurality of polyvinylpyrrolidone (PVP) polymer chains covalently attached to the solid support. The polyvinylpyrrolidone polymer chains are either vinylpyrrolidone homopolymer chains or copolymer chains which comprise at least 70 mol % vinylpyrrolidone monomer residues and less than 2 mol % negatively charged monomer residues.

Abstract translation: 本发明公开了具有多孔固体支持物和共价连接到固体支持物上的多个聚乙烯吡咯烷酮（PVP）聚合物链的分离基质。 聚乙烯吡咯烷酮聚合物链是乙烯基吡咯烷酮均聚物链或包含至少70mol％乙烯基吡咯烷酮单体残基和小于2mol％带负电荷的单体残基的共聚物链。