公开(公告)号：US20190185917A1

公开(公告)日：2019-06-20

申请号：US16222791

申请日：2018-12-17

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Jijumon CHELLISERRY

IPC: C12Q1/6811 ,  C12Q1/6848 ,  C12Q1/6844 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q1/6869

CPC classification number: C12Q1/6811 ,  C07H21/00 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2537/1373

Abstract: The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.

公开(公告)号：US20180143115A1

公开(公告)日：2018-05-24

申请号：US15573014

申请日：2016-05-12

Applicant: GEN-PROBE INCORPORATED

Inventor： Jeffrey M. LINNEN ,  Jijumon CHELLISERRY

IPC: G01N1/38 ,  C12Q1/6893 ,  C12Q1/6806 ,  C12Q1/70

CPC classification number: G01N1/38 ,  C12N1/06 ,  C12N15/1003 ,  C12Q1/04 ,  C12Q1/6806 ,  C12Q1/6893 ,  C12Q1/70

Abstract: The disclosure provides methods of pooled analysis of samples containing red blood cells, such as whole blood cell samples. The methods are particularly useful for screening whole blood samples collected from many individuals to be used for transfusions and the like. Aliquots of such samples are individually contacted with a lysis reagent, further described below, before pooling to lyse red blood cells in the samples. After pooling, the lysates, the combined lysate is tested for presence of target(s) characteristic of pathogen(s). The method are particularly useful for identifying pathogens of red blood cells, but can also be used to nm a complete panel of testing including for all of the typical targets.

公开(公告)号：US20160201144A1

公开(公告)日：2016-07-14

申请号：US14918131

申请日：2015-10-20

Applicant: Gen-Probe Incorporated

Inventor： Jijumon CHELLISERRY ,  Kui GAO ,  Jeffrey M. LINNEN

IPC: C12Q1/68

CPC classification number: C12Q1/6893 ,  C12N1/06 ,  C12Q1/6806 ,  C12Q2600/158 ,  C12Q2527/125

Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

Abstract translation: 本发明提供了用于裂解红细胞的裂解试剂，从而以适合于分析的形式从寄生生物体释放靶，例如RNA。 试剂至少包括氯化铵和阴离子洗涤剂，并且可以包括抗凝剂。 该试剂用于裂解红细胞，保护释放的靶免受裂解物中的降解，并且与目标捕获，扩增，检测或测序等目标分析的后续步骤兼容。

公开(公告)号：US20140066326A1

公开(公告)日：2014-03-06

申请号：US14014607

申请日：2013-08-30

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Lyle J. ARNOLD, JR. ,  Lizhong DAI ,  Steven PHELPS ,  Jijumon CHELLISERRY

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q1/70 ,  C12Q2537/101 ,  C12Q2545/10

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

Abstract translation: 公开了用于核酸扩增的改进方法，包括多重扩增，其中扩增在两个或多个不同阶段中进行。 第一相扩增反应优选缺少指数扩增所需的一种或多种组分。 随后在第二，第三或另外的扩增相中提供缺乏的组分，导致快速指数扩增反应。 多相协议在低分析物浓度下导致更快更灵敏的检测和更低的变异性。 还公开了用于实施所要求保护的方法的组合物。