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公开(公告)号：US20070148762A1
公开(公告)日：2007-06-28
申请号：US10589376
申请日：2005-02-18
IPC分类号： C12M3/00
CPC分类号： C12N5/0068 , C12N2533/30 , C12N2535/10
摘要： A cell culture patterning substrate on which cells can be arranged regularly with high efficiency over a large area on a base material so as to attain formation of a tissue, etc. The cell culture patterning substrate includes: a base material; and a cell culture region which is formed on the base material, is a region for culturing a cell and contains a cell adhesive layer having adhesive properties to the cell. The cell culture region includes: a cell adhesion portion at which the cell adhesive layer is formed; and a cell adhesion auxiliary portion, formed in a pattern, which inhibits adhesion to the cell, and the cell adhesion auxiliary portion is formed such that, upon adhesion of the cell to the cell adhesion portion, the cells on two cell adhesion portions adjacent to the cell adhesion auxiliary portion can be bound to each other on the cell adhesion auxiliary portion.
摘要翻译： 一种细胞培养图案形成基板，其上可以在基材上大面积地高效率地排列细胞以形成组织等。细胞培养图案形成基板包括：基材; 并且在基材上形成的细胞培养区是用于培养细胞的区域，并且含有对细胞具有粘合性的细胞粘合剂层。 细胞培养区域包括：形成细胞粘合剂层的细胞粘附部分; 和形成为抑制与细胞粘合的图案的细胞粘附辅助部分和细胞粘附辅助部分形成，使得当细胞粘附到细胞粘附部分时，细胞粘附在两个细胞粘附部分上 细胞粘附辅助部分可以在细胞粘附辅助部分上彼此结合。
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公开(公告)号：US20050255594A1
公开(公告)日：2005-11-17
申请号：US11044437
申请日：2005-01-27
CPC分类号： C12N5/0068 , C12N2533/12 , C12N2533/30 , C12N2535/10
摘要： The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells is adhered in a highly precise pattern. In order to attain the object, the invention provides a patterning substrate comprising a base material, a photocatalyst-containing layer which is formed on the base material and comprises at least a photocatalyst, and a cell adhesion-inhibiting layer which is formed on the photocatalyst-containing layer and comprises a cell adhesion-inhibiting material that has cell adhesion-inhibiting properties of inhibiting adhesion to cells and is decomposed or denatured by action of the photocatalyst on the basis of irradiation with energy.
摘要翻译： 本发明主要提供用于将细胞以高精度图案粘附到培养基材上的细胞培养图案形成基材和以高精度图案粘附细胞的细胞培养基质。 为了达到上述目的，本发明提供一种图案形成基材，其包含基材，含有光催化剂的层，其形成在基材上，并且至少包含光催化剂，以及在光催化剂上形成的细胞粘附抑制层 并且包含细胞粘附抑制材料，其具有抑制细胞粘附的细胞粘附抑制性质，并且通过基于光能照射的光催化剂的作用而分解或变性。
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公开(公告)号：US09034648B2
公开(公告)日：2015-05-19
申请号：US13924181
申请日：2013-06-21
CPC分类号： C12N5/0691 , A61L27/36 , A61L27/3886 , A61L2430/36 , C12N5/0697
摘要： An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
摘要翻译： 本发明的目的是提供一种人造组织结构，其具有用于运输营养物，氧气，废物等的手段，并且在体内是可行的。 本发明涉及体外形成的组织构建体，其包括血管层，基底膜层和组织形成细胞层。
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公开(公告)号：US20100330665A1
公开(公告)日：2010-12-30
申请号：US12881492
申请日：2010-09-14
IPC分类号： C12M1/26
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells.To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。
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公开(公告)号：US20080213857A1
公开(公告)日：2008-09-04
申请号：US12002863
申请日：2007-12-19
IPC分类号： C12N11/14
CPC分类号： C12N5/0068 , C12N2533/12 , C12N2533/30 , C12N2535/10
摘要： The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells is adhered in a highly precise pattern.In order to attain the object, the invention provides a patterning substrate comprising a base material, a photocatalyst-containing layer which is formed on the base material and comprises at least a photocatalyst, and a cell adhesion-inhibiting layer which is formed on the photocatalyst-containing layer and comprises a cell adhesion-inhibiting material that has cell adhesion-inhibiting properties of inhibiting adhesion to cells and is decomposed or denatured by action of the photocatalyst on the basis of irradiation with energy.
摘要翻译： 本发明主要提供用于将细胞以高精度图案粘附到培养基材上的细胞培养图案形成基材和以高精度图案粘附细胞的细胞培养基质。 为了达到上述目的，本发明提供一种图案形成基材，其包含基材，含有光催化剂的层，其形成在基材上，并且至少包含光催化剂，以及在光催化剂上形成的细胞粘附抑制层 并且包含细胞粘附抑制材料，其具有抑制细胞粘附的细胞粘附抑制性质，并且通过基于光能照射的光催化剂的作用而分解或变性。
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公开(公告)号：US20050279730A1
公开(公告)日：2005-12-22
申请号：US11062674
申请日：2005-02-22
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附在基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。
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公开(公告)号：US20080124795A1
公开(公告)日：2008-05-29
申请号：US10592199
申请日：2005-03-10
申请人： Hideyuki Miyake , Hideshi Hattori
发明人： Hideyuki Miyake , Hideshi Hattori
IPC分类号： C12N5/02
CPC分类号： C12N5/0068 , A61L27/3804 , C12N2533/30 , C12N2535/10
摘要： A main object of the present invention is to provide a vascular cell culture patterning substrate, which can efficiently form a plurality of blood vessels on one substrate. To achieve the object, the present invention provides a vascular cell culture patterning substrate comprising: a base material; a vascular cell adhesion portion formed in at least two substantially parallel lines on the base material, and has an adhesive properties to a vascular cell which forms a blood vessel; and a vascular cell adhesion-inhibiting portion formed in between two adjacent vascular cell adhesion portions on the base material, and inhibits adhesion to the vascular cell,wherein the vascular cell adhesion-inhibiting portions are formed at such a surface distance that, upon adhesion of the vascular cells to the vascular cell adhesion portions, the cells on the two vascular cell adhesion portions adjacent to the vascular cell adhesion-inhibiting portion are not contacted with one another via pseudopods.
摘要翻译： 本发明的主要目的在于提供能够在一个基板上有效地形成多个血管的血管细胞培养图案形成基板。 为了实现该目的，本发明提供一种血管细胞培养图案形成基材，其包含：基材; 形成在所述基材上的至少两条基本平行的线上的血管细胞粘附部分，并且对形成血管的血管细胞具有粘合性; 以及形成在基材上的两个相邻的血管细胞粘附部分之间的血管细胞粘附抑制部分，并且抑制对血管细胞的粘附，其中所述血管细胞粘附抑制部分形成在表面距离处， 血管细胞与血管细胞粘附部分相邻，血管细胞粘附抑制部分附近的两个血管细胞粘附部分上的细胞不经由伪足相互接触。
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公开(公告)号：US20070243613A1
公开(公告)日：2007-10-18
申请号：US11709119
申请日：2007-02-20
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。
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19.发明申请公开(公告)号：US20070122901A1
公开(公告)日：2007-05-31
申请号：US10574687
申请日：2004-10-15
申请人： Ikuo Morita , Makoto Nakamura , Hideyuki Miyake , Hideshi Hattori , Hironori Kobayashi , Masaaki Kurihara
发明人： Ikuo Morita , Makoto Nakamura , Hideyuki Miyake , Hideshi Hattori , Hironori Kobayashi , Masaaki Kurihara
IPC分类号： C12N5/06
CPC分类号： C12N5/0068 , A61L27/38 , C12N2533/00
摘要： The present invention relates to a method for culturing cells, which comprises the steps of: causing cells to adhere to the surface of a cell array substrate having a cell adhesiveness variation pattern that comprises regions having good cell adhesiveness and regions having inhibited cell adhesiveness patterned on a substrate; transferring the adhered cells to a cell culture substrate in such patterned state; and culturing the transferred cells.
摘要翻译： 本发明涉及一种培养细胞的方法，包括以下步骤：使细胞粘附到具有细胞粘附性变化图案的细胞阵列基板的表面，所述细胞粘附变化图案包含具有良好细胞粘附性的区域和图案化的细胞粘附性抑制区域 底物; 以这样的图案化状态将粘附的细胞转移到细胞培养基质; 并培养转移的细胞。
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公开(公告)号：US20060183219A1
公开(公告)日：2006-08-17
申请号：US11044124
申请日：2005-01-27
CPC分类号： C12Q1/045 , C12N5/0068 , C12N2533/30 , C12N2533/40
摘要： The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells are adhered in a highly precise pattern. To attain the object, the invention provides a patterning substrate comprising: a base material; and a cell adhesion-inhibiting layer which is formed on the base material and comprises a cell adhesion-inhibiting material that has cell adhesion-inhibiting properties of inhibiting adhesion to cells and is denatured by action of a photocatalyst on the basis of irradiation with energy.
摘要翻译： 本发明主要提供用于将细胞以高精度图案粘附到培养基材上的细胞培养图案形成基板和以高精度图案粘附细胞的细胞培养基板。 为了实现该目的，本发明提供了一种图案化衬底，包括：基材; 以及细胞粘附抑制层，其形成在基材上，并且包含细胞粘附抑制材料，其具有抑制细胞粘附的细胞粘附抑制性质，并且通过基于光能照射的光催化剂的作用而变性。
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