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公开(公告)号：US08497117B2
公开(公告)日：2013-07-30
申请号：US13032998
申请日：2011-02-23
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells.To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分的含细胞的液体施加方法。
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公开(公告)号：US07919305B2
公开(公告)日：2011-04-05
申请号：US11062674
申请日：2005-02-22
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells.To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分的含细胞的液体施加方法。
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公开(公告)号：US20100330665A1
公开(公告)日：2010-12-30
申请号：US12881492
申请日：2010-09-14
IPC分类号： C12M1/26
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells.To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。
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公开(公告)号：US20050279730A1
公开(公告)日：2005-12-22
申请号：US11062674
申请日：2005-02-22
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附在基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。
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公开(公告)号：US20070243613A1
公开(公告)日：2007-10-18
申请号：US11709119
申请日：2007-02-20
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译： 本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。 为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部， 通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。
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公开(公告)号：US08500822B2
公开(公告)日：2013-08-06
申请号：US11628054
申请日：2005-05-31
IPC分类号： A61F2/02
CPC分类号： C12N5/0691 , A61L27/36 , A61L27/3886 , A61L2430/36 , C12N5/0697
摘要： An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
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7.发明授权Method for producing cell culture substrate and apparatus for producing cell culture substrate 失效细胞培养基质的制造方法和细胞培养基材的制造装置公开(公告)号：US07687251B2
公开(公告)日：2010-03-30
申请号：US10594174
申请日：2005-03-24
CPC分类号： C12M25/06 , C12M33/00 , C12N5/0068 , C12N2535/10 , Y10S977/703
摘要： A method for producing a cell culture substrate to which cells adhere in a finely processed pattern while retaining the pattern for a long period of time to culture the cells and a production apparatus used in the production method. The method includes: forming a patterning substrate by forming: on a base material, a light shielding portion, and a cell adhesive layer having adhesion to a cell and containing a cell adhesive material which is decomposed or denatured by an action of a photocatalyst upon energy irradiation so as to cover the light shielding portion; an energy irradiating process of irradiating energy to the patterning substrate from the base material side to form a pattern; and a cell adhesion process of making the cell adhere to the cell adhesion portion in a cell culture medium containing the cell and a culture medium.
摘要翻译： 细胞培养基板的制造方法，其中细胞以精细加工的图案粘附，同时长期保持图案以培养细胞，以及生产方法中使用的制造装置。 该方法包括：通过在基底材料，遮光部分和具有对细胞粘附的细胞粘合剂层上形成图案形成衬底，并且含有通过光催化剂的作用而被分解或变性的细胞粘附材料的能量 照射以覆盖遮光部分; 能量照射处理，其从所述基材侧向所述图案化衬底照射能量以形成图案; 以及在含有细胞和培养基的细胞培养基中使细胞粘附于细胞粘附部的细胞粘附过程。
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公开(公告)号：US20070259328A1
公开(公告)日：2007-11-08
申请号：US11628054
申请日：2005-05-31
CPC分类号： C12N5/0691 , A61L27/36 , A61L27/3886 , A61L2430/36 , C12N5/0697
摘要： An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
摘要翻译： 本发明的目的是提供一种人造组织结构，其具有用于运输营养物，氧气，废物等的手段，并且在体内是可行的。 本发明涉及体外形成的组织构建体，其包括血管层，基底膜层和组织形成细胞层。
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公开(公告)号：US20050186674A1
公开(公告)日：2005-08-25
申请号：US10986641
申请日：2004-11-12
CPC分类号： C12N5/0068 , C12M25/00 , C12N2535/10
摘要： The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere a cell in a highly precise pattern on a base material to culture and a cell culture substrate on which a cell is adhered in a high precision pattern. In order to achieve the above-mentioned object, the present invention provides a patterning substrate having a base material and a cell culture patterning layer that is formed on the base material and has at least a photocatalyst and a cell adhesive material that has the adhesiveness with the cell and is decomposed or modified owing to an action of the photocatalyst in combination with energy irradiation.
摘要翻译： 本发明主要提供用于将细胞以高精度图案附着在基材上培养的细胞培养图案形成基板和以高精度图案粘附细胞的细胞培养基板。 为了实现上述目的，本发明提供一种图案形成基板，其具有基材和细胞培养图案形成层，所述基材和细胞培养图案化层形成在所述基材上，并且至少具有光催化剂和具有与 由于光催化剂的作用与能量照射的结合而被分解或改性。
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10.发明申请公开(公告)号：US20070218554A1
公开(公告)日：2007-09-20
申请号：US11587363
申请日：2005-04-25
IPC分类号： C12N5/02
CPC分类号： C12N5/0068 , C12M23/20 , C12M25/06 , C12M33/00 , C12N2535/10 , C12N2539/10
摘要： A main object of the present invention is to provide a cell culture patterning substrate and a method for manufacturing the same, which can be used to culture the cells on the substrate in an intended shape. To achieve the object, the present invention provides a cell culture patterning substrate comprising: a base material provided with a convex portion; and a cell culture region, which is a region for culturing a cell, formed on a surface of the base material, wherein the cell culture region is partitioned with the convex portion of the base material provided with the convex portion.
摘要翻译： 本发明的主要目的在于提供一种细胞培养图案形成基板及其制造方法，其可以用于将基板上的细胞以期望的形状培养。 为了达到上述目的，本发明提供一种细胞培养图案形成基板，其特征在于，具备：设置有凸部的基材; 和形成在基材表面上的细胞培养区域的细胞培养区域，其中细胞培养区域与设置有凸部的基材的凸部分隔开。
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