公开(公告)号：US09994618B2

公开(公告)日：2018-06-12

申请号：US14418004

申请日：2013-07-30

Applicant: Hong-Jin Kim

Inventor： Hong-Jin Kim ,  Hyoung Jin Kim

IPC: C07K14/005 ,  C07K14/025 ,  C12N7/00 ,  C12N7/02 ,  A61K39/00 ,  A61K39/12

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N7/00 ,  C12N2710/20034 ,  C12N2710/20051

Abstract: Provided is a method of purifying human papillomavirus (HPV) L1 proteins with high purity and high efficiency. According to the purification method, a purification purity and yield of HPV L1 proteins can be considerably increased when heating/chilling is formed by treating a cell homogenate with a reducing agent. In addition, VLPs of the HPV L1 protein purified by the purification method have excellent antigenicity and immunogenicity.

公开(公告)号：US20150266927A1

公开(公告)日：2015-09-24

申请号：US14418004

申请日：2013-07-30

Applicant: Hong-Jin KIM

Inventor： Hong-Jin Kim ,  Hyoung Jin Kim

IPC: C07K14/005 ,  C12N7/00

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N7/00 ,  C12N2710/20034 ,  C12N2710/20051

Abstract: Provided is a method of purifying human papillomavirus (HPV) L1 proteins with high purity and high efficiency. According to the purification method, a purification purity and yield of HPV L1 proteins can be considerably increased when heating/chilling is formed by treating a cell homogenate with a reducing agent. In addition, VLPs of the HPV L1 protein purified by the purification method have excellent antigenicity and immunogenicity.

Abstract translation: 提供了一种纯化和高效率纯化人乳头状瘤病毒（HPV）L1蛋白的方法。 根据纯化方法，当通过用还原剂处理细胞匀浆而加热/冷却时，可以显着增加HPV L1蛋白的纯化纯度和产率。 此外，通过纯化方法纯化的HPV L1蛋白的VLP具有优异的抗原性和免疫原性。

公开(公告)号：US08597081B2

公开(公告)日：2013-12-03

申请号：US13241421

申请日：2011-09-23

Applicant: Jae-Kwang Choi ,  Hong-Jin Kim ,  Keon-Sik Seo ,  Sol Han ,  Kun-Tack Lee ,  Byoung-Ho Kwon

Inventor： Jae-Kwang Choi ,  Hong-Jin Kim ,  Keon-Sik Seo ,  Sol Han ,  Kun-Tack Lee ,  Byoung-Ho Kwon

IPC: B24B1/00

CPC classification number: B24B53/017

Abstract: A pad conditioning disk, a pre-conditioning unit, and a CMP apparatus having the same are provided. The pad conditioning disk includes a base in which mountain-type tips and valley-type grooves are repeatedly connected to each other, and a cutting layer formed on the base layer. The cutting layer including conditioning particles deposited on surfaces of the tips and grooves. A surfaces roughness of conditioning particles deposited on the surfaces of the tips is less than a surface roughness of conditioning particles deposited on the surfaces of the grooves.

Abstract translation: 提供了衬垫调节盘，预调节单元和具有该调节盘的CMP设备。 垫调节盘包括其中山型尖端和谷型沟槽彼此重复连接的基底和形成在基底层上的切割层。 切割层包括沉积在尖端和凹槽表面上的调节颗粒。 沉积在尖端表面上的调理颗粒的表面粗糙度小于沉积在凹槽表面上的调理颗粒的表面粗糙度。

公开(公告)号：US20120100169A1

公开(公告)日：2012-04-26

申请号：US13379212

申请日：2009-10-20

Applicant: Hong-Jin Kim ,  Na Gyong Lee ,  Yang-Je Cho ,  Jin-Wook Jang ,  Hyoung Jin Kim ,  Kwang Sung Kim

Inventor： Hong-Jin Kim ,  Na Gyong Lee ,  Yang-Je Cho ,  Jin-Wook Jang ,  Hyoung Jin Kim ,  Kwang Sung Kim

IPC: A61K39/12 ,  A61P35/00 ,  C12N1/19 ,  A61P37/04 ,  C12P21/02 ,  C12N15/37

CPC classification number: A61K39/12 ,  A61K39/39 ,  A61K2039/5258 ,  A61K2039/55505 ,  A61K2039/55572 ,  A61K2039/70 ,  C12N2710/20023 ,  C12N2710/20034

Abstract: The present invention relates to a pharmaceutical vaccine composition for a human cervical cancer, comprising: (a) (i) a L1 virus-like particle (VLP) of human papillomavirus (HPV) type 16, a L1 VLP of HPV type 18, or a combination thereof; and (ii) a deacylated non-toxic lipooligosaccharide (LOS); and (b) a pharmaceutically acceptable carrier; and a method for preparing a human papillomavirus (HPV) L1 virus-like particle (VLP). The pharmaceutical vaccine composition of the present invention is in both Th1-type immune response (cellular immunity) and Th2-type immune response (humoral immunity) against HPV more excellent than Cervrix™ and Gardasil™, exhibiting a superior efficacy as a vaccine for a human cervical cancer.

Abstract translation: 本发明涉及用于人宫颈癌的药物疫苗组合物，其包括：（i）（i）16型人乳头状瘤病毒（HPV）的L1病毒样颗粒（VLP），HPV 18型的L1 VLP或 其组合; 和（ii）脱酰无毒低密度寡糖（LOS）; 和（b）药学上可接受的载体; 以及制备人乳头状瘤病毒（HPV）L1病毒样颗粒（VLP）的方法。 本发明的药物疫苗组合物具有比Cervrix TM和Gardasil TM更优异的Th1型免疫应答（细胞免疫）和对HPV的Th2型免疫应答（体液免疫），表现出作为疫苗的优异功效 人宫颈癌。

公开(公告)号：US07118913B2

公开(公告)日：2006-10-10

申请号：US10363421

申请日：2001-08-24

Applicant: Hong-Jin Kim ,  Myung-II Chung ,  Ik-Hwan Kim ,  Ick-Young Kim

Inventor： Hong-Jin Kim ,  Myung-II Chung ,  Ik-Hwan Kim ,  Ick-Young Kim

IPC: C12N5/06 ,  C12N15/63

CPC classification number: C12N9/1018 ,  C12P21/00

Abstract: The present invention relates to expression vectors containing in vivo urea cycle enzyme gene; transformants thereof; and the use of transformants for protein overexpression. During the overexpression of protein, if the animal cell transformed with expression vectors of the present invention is used, amounts of ammonia accumulation within cell culture medium will decrease and cell growth rate will increase, thus, it is advantageous to be capable of obtaining desired protein in high yields.

Abstract translation: 本发明涉及含有体内尿素循环酶基因的表达载体; 转化体 并使用转化体进行蛋白质过表达。 在蛋白质的过表达中，如果使用本发明的表达载体转化的动物细胞，则细胞培养液中的氨积累量将降低，细胞生长速度增加，因此有利的是能够获得所需的蛋白质 产量高。

公开(公告)号：US06917405B2

公开(公告)日：2005-07-12

申请号：US10918456

申请日：2004-08-16

Applicant: Hong-Jin Kim

Inventor： Hong-Jin Kim

IPC: G02F1/1368 ,  G02F1/1335 ,  G02F1/1362 ,  H01L21/336 ,  H01L29/786 ,  G02F1/1343

CPC classification number: G02F1/133555 ,  G02F1/136227 ,  G02F2001/13629

Abstract: An array substrate for a transflective liquid crystal display device includes: a gate line on a substrate; a data line crossing the gate line, the gate line and the data line defining a pixel region having a transmissive portion and a reflective portion; a gate electrode connected to the gate line; source and drain electrode spaced apart from each other over the gate electrode, the source and drain electrode being spaced apart from each other, the source electrode being connected to the data line; a reflective layer having the same layer as the source and drain electrodes, the reflective layer being disposed in the pixel region and having a transmissive hole corresponding to the transmissive portion; and a pixel electrode connected to the drain electrode, the pixel electrode being disposed in the pixel region, wherein the source and drain electrodes and the reflective layer have multiple layers of metal, wherein a top layer of the multiple layers includes a reflective metallic material.

Abstract translation: 一种半透射型液晶显示装置的阵列基板，包括：基板上的栅极线; 与栅极线交叉的数据线，栅极线和数据线限定具有透射部分和反射部分的像素区域; 连接到栅极线的栅电极; 源极和漏极彼此间隔开，源极和漏极彼此间隔开，源电极连接到数据线; 反射层，其具有与源电极和漏电极相同的层，反射层设置在像素区域中并且具有与透射部分相对应的透射孔; 以及连接到所述漏电极的像素电极，所述像素电极设置在所述像素区域中，其中所述源电极和漏电极以及所述反射层具有多层金属，其中所述多层的顶层包括反射金属材料。

公开(公告)号：US20050012110A1

公开(公告)日：2005-01-20

申请号：US10918456

申请日：2004-08-16

Applicant: Hong-Jin Kim

Inventor： Hong-Jin Kim

IPC: G02F1/1368 ,  G02F1/1335 ,  G02F1/1362 ,  H01L21/336 ,  H01L29/786 ,  H01L29/74 ,  H01L31/111

CPC classification number: G02F1/133555 ,  G02F1/136227 ,  G02F2001/13629

Abstract: An array substrate for a transflective liquid crystal display device includes: a gate line on a substrate; a data line crossing the gate line, the gate line and the data line defining a pixel region having a transmissive portion and a reflective portion; a gate electrode connected to the gate line; source and drain electrode spaced apart from each other over the gate electrode, the source and drain electrode being spaced apart from each other, the source electrode being connected to the data line; a reflective layer having the same layer as the source and drain electrodes, the reflective layer being disposed in the pixel region and having a transmissive hole corresponding to the transmissive portion; and a pixel electrode connected to the drain electrode, the pixel electrode being disposed in the pixel region, wherein the source and drain electrodes and the reflective layer have multiple layers of metal, wherein a top layer of the multiple layers includes a reflective metallic material.

Abstract translation: 一种半透射型液晶显示装置的阵列基板，包括：基板上的栅极线; 与栅极线交叉的数据线，栅极线和数据线限定具有透射部分和反射部分的像素区域; 连接到栅极线的栅电极; 源极和漏极彼此间隔开，源极和漏极彼此间隔开，源电极连接到数据线; 反射层，其具有与源电极和漏电极相同的层，反射层设置在像素区域中并且具有与透射部分相对应的透射孔; 以及连接到所述漏电极的像素电极，所述像素电极设置在所述像素区域中，其中所述源电极和漏电极以及所述反射层具有多层金属，其中所述多层的顶层包括反射金属材料。

公开(公告)号：US06651010B1

公开(公告)日：2003-11-18

申请号：US09210305

申请日：1998-12-11

Applicant: Kyungsook Han ,  Dohyung Kim ,  Hong-Jin Kim

Inventor： Kyungsook Han ,  Dohyung Kim ,  Hong-Jin Kim

IPC: G06F1900

CPC classification number: G06F19/26 ,  G06F19/16

Abstract: There is disclosed a method for visualizing secondary structures of RNA molecules, by which nearly overlap-free polygonal displays of RNA secondary structures are produced with minimal distortion to structural elements, with minimal search for positioning them, and with minimal user intervention. While vector and vector space are used to determine the direction and space of a structural element, two heuristics are adopted for the task of searching for the space and direction of structural elements. With the aid of the two heuristics, loops are positioned in decreasing order of their sizes and a helix is positioned, depending on the position of its adjacent loop which has been positioned. In consideration of both a potentially open and wide vector space and an allowed vector space, a structural element is positioned.

Abstract translation: 公开了一种用于可视化RNA分子的二级结构的方法，通过该方法，以最小的搜索定位它们并且以最小的用户干预，以最小的变形结构元素产生RNA二级结构的几乎无重叠的多边形显示。 虽然使用向量和向量空间来确定结构元素的方向和空间，但是为了搜索结构元素的空间和方向，采用了两种启发式方法。 借助于这两种启发式方式，环路按其尺寸的降序排列，根据已定位的相邻环的位置，定位螺旋线。 考虑到潜在开放和宽的向量空间和允许的向量空间，定位了结构元件。