公开(公告)号：US10053730B2

公开(公告)日：2018-08-21

申请号：US14817639

申请日：2015-08-04

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869

CPC classification number: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2565/501 ,  C12Q2537/143

Abstract: A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

公开(公告)号：US09649614B2

公开(公告)日：2017-05-16

申请号：US14726325

申请日：2015-05-29

Applicant: ILLUMINA, INC.

Inventor： Michal Lebl ,  Michel Perbost ,  Chad F. Derosier ,  Mark J. Nibbe ,  Steve R. Burgett ,  David L. Heiner

IPC: B01L3/00 ,  B01J19/22 ,  B01J14/00 ,  B01J19/00 ,  B01L7/00

CPC classification number: B01J19/22 ,  B01J14/005 ,  B01J19/0046 ,  B01J2219/00306 ,  B01J2219/00324 ,  B01J2219/00351 ,  B01J2219/0036 ,  B01J2219/00378 ,  B01J2219/0038 ,  B01J2219/00495 ,  B01J2219/00518 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00644 ,  B01J2219/00686 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01L3/50851 ,  B01L7/5255 ,  B01L2300/069 ,  B01L2300/0812 ,  B01L2300/0829 ,  B01L2300/123

Abstract: Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers.

公开(公告)号：US20150072871A1

公开(公告)日：2015-03-12

申请号：US14539765

申请日：2014-11-12

Applicant: ILLUMINA, INC.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/501 ,  C12Q2537/143

Abstract: A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

Abstract translation: 公开了以自动或半自动方式测序核酸的技术。 多个核酸位点的样品阵列在多个周期内进行处理以向要测序的材料添加核苷酸，检测添加到位点的核苷酸，以及去除阻断剂的添加核苷酸和用于鉴定最后添加的标签 核苷酸。 对系统的多个参数进行监控，以便在样品排序期间发生问题的诊断和纠正。 质量控制程序在排序过程中运行，以确定样品的质量和收集的数据的质量。