公开(公告)号：US10723995B1

公开(公告)日：2020-07-28

申请号：US16820292

申请日：2020-03-16

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/12 ,  B01D25/00 ,  B01D63/08 ,  C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00 ,  B01D69/06 ,  B01D71/02

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200216795A1

公开(公告)日：2020-07-09

申请号：US16823269

申请日：2020-03-18

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20220333273A1

公开(公告)日：2022-10-20

申请号：US17728735

申请日：2022-04-25

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US11268061B2

公开(公告)日：2022-03-08

申请号：US17401362

申请日：2021-08-13

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M3/00 ,  C12M1/00 ,  B01L3/00 ,  C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US11085131B1

公开(公告)日：2021-08-10

申请号：US17232046

申请日：2021-04-15

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C12N9/22 ,  C40B50/06 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US11078458B2

公开(公告)日：2021-08-03

申请号：US17159137

申请日：2021-01-26

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held ,  Eric Abbate ,  Michael Clay ,  Katherine Krouse ,  Nandini Krishnamurthy ,  Krishna Yerramsetty

IPC: C12N1/20 ,  C07K14/245

Abstract: The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.

公开(公告)号：US11046928B2

公开(公告)日：2021-06-29

申请号：US17102414

申请日：2020-11-23

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00 ,  B01D63/08 ,  B01D69/06 ,  B01D71/02

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US10995424B2

公开(公告)日：2021-05-04

申请号：US16950878

申请日：2020-11-17

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C12N9/22 ,  C40B50/06 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US20210095394A1

公开(公告)日：2021-04-01

申请号：US16950878

申请日：2020-11-17

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US20210095243A1

公开(公告)日：2021-04-01

申请号：US17026359

申请日：2020-09-21

Applicant: Inscripta, Inc.

Inventor： Bryan Leland ,  Michael Clay ,  Eric Abbate ,  Stephen Federowicz ,  Richard Fox

IPC: C12N1/14 ,  C12N15/10 ,  C12N9/10

Abstract: The present disclosure relates to various different types of mutations in S. cerevisiae leading to enhanced tyrosine production for, e.g., supplements and nutraceuticals.