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37 条记录 (耗时 0.038 秒)

    Systems and methods for a DNA-based thermometer
    13.
    发明授权
    Systems and methods for a DNA-based thermometer 有权
    基于DNA的温度计的系统和方法

    公开(公告)号：US09194801B2

    公开(公告)日：2015-11-24

    申请号：US13595106

    申请日：2012-08-27

    申请人： Kenneth L. Shepard ,  Ioannis Kymissis ,  Haig Norian

    发明人： Kenneth L. Shepard ,  Ioannis Kymissis ,  Haig Norian

    IPC分类号： G01N25/00 ,  G01N21/64 ,  B01J19/00 ,  G01K11/20

    CPC分类号： G01N21/6486 ,  B01J19/0046 ,  G01K11/20 ,  G01K2213/00 ,  G01N21/6428

    摘要： DNA-based temperature sensor for measuring temperature through a transition of one or more strands of DNA from a coupled configuration to a decoupled configuration at a temperature threshold, and a fluorescent dye adapted to emit fluorescence when the DNA is in the coupled configuration, includes a receptacle adapted to receive the DNA and the fluorescent dye in a solution, an imaging device adapted to acquire an image of fluorescence emitted from the solution, the image having a plurality of regions, and a processor adapted to determine a plurality of fluorescence levels corresponding to each of the plurality of regions of the image and to generate a temperature map based on the determined fluorescence levels. A method for measuring temperature and a DNA-based temperature sensing solution are also provided.

    摘要翻译： 基于DNA的温度传感器，用于通过一个或多个DNA链从温度阈值的耦合配置转变为解耦配置来测量温度，以及当DNA处于耦合配置时适于发射荧光的荧光染料包括： 适于在溶液中接收DNA和荧光染料的容器，适于获取从溶液发射的荧光图像的成像装置，具有多个区域的图像，以及适于确定对应于多个区域的多个荧光水平的处理器 每个图像的多个区域，并且基于所确定的荧光水平生成温度图。 还提供了一种测量温度的方法和基于DNA的温度感测解决方案。

    Active CMOS Sensor Array For Electrochemical Biomolecular Detection
    14.
    发明申请
    Active CMOS Sensor Array For Electrochemical Biomolecular Detection 审中-公开
    有源CMOS传感器阵列用于电化学生物分子检测

    公开(公告)号：US20100300899A1

    公开(公告)日：2010-12-02

    申请号：US12819720

    申请日：2010-06-21

    申请人： Peter M. Levine ,  Kenneth L. Shepard ,  Ping Gong ,  Levicky Rastislav

    发明人： Peter M. Levine ,  Kenneth L. Shepard ,  Ping Gong ,  Levicky Rastislav

    IPC分类号： G01N27/26 ,  G01N27/28 ,  H01L21/30

    CPC分类号： G01N27/3277 ,  C12Q1/6825 ,  G01N27/48 ,  H01L23/5226 ,  H01L23/5283 ,  H01L23/53261

    摘要： Electrochemical sensing of biomolecules eliminates the need for bulky optical instruments required in traditional fluorescence-based sensing assays. Integration of the sensor interface electrodes and active electrochemical detection circuitry on CMOS substrates miniaturizes the sensing platform, enhancing portability for point-of-care applications, while enabling high-throughput, highly-parallel analysis. One embodiment includes a four-by-four active sensor array for multiplexed electrochemical biomolecular detection in a standard 0.25-μm CMOS process. Integrated potentiostats, including control amplifiers and dual-slope ADCs, stimulate the electrochemical cell and detect the current flowing through on-chip gold electrodes at each sensor site resulting from biomolecular reactions occurring on the chip surface. Post-processing techniques for fabricating biologically-compatible surface-electrode arrays in CMOS that can withstand operation in harsh electrochemical environments are described. Demonstrations showing example operation of the active CMOS array for biomolecular detection include cyclic voltammetry of a reversible redox species, DNA probe density characterization, and quantitative and specific DNA hybridization detection in real time.

    摘要翻译： 生物分子的电化学感测消除了对传统基于荧光的感测测定中所需的庞大的光学仪器的需要。 传感器接口电极和主动电化学检测电路在CMOS基板上的集成使传感平台小型化，增强了点对点应用的便携性，同时实现了高吞吐量，高度平行的分析。 一个实施例包括用于在标准0.25μmCMOS工艺中多路复用电化学生物分子检测的四乘四活性传感器阵列。 集成的恒电位仪，包括控制放大器和双斜率ADC，可激发电化学电池，并检测流经芯片表面上生物分子反应的每个传感器位置的片上金电极的电流。 描述了用于在CMOS中制造可承受恶劣电化学环境中的操作的生物相容的表面电极阵列的后处理技术。 显示用于生物分子检测的有源CMOS阵列的示例操作的演示包括可逆氧化还原物质的循环伏安法，DNA探针密度表征和实时定量和特异性DNA杂交检测。

    Active CMOS biosensor chip for fluorescent-based detection
    15.
    发明授权
    Active CMOS biosensor chip for fluorescent-based detection 有权
    有源CMOS生物传感器芯片，用于荧光检测

    公开(公告)号：US07738086B2

    公开(公告)日：2010-06-15

    申请号：US11800468

    申请日：2007-05-04

    申请人： Kenneth L. Shepard ,  Rastislav Levicky ,  George Patounakis

    发明人： Kenneth L. Shepard ,  Rastislav Levicky ,  George Patounakis

    IPC分类号： G01N21/00

    CPC分类号： H04N5/37452 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6454 ,  H01L27/14621 ,  H01L27/14643

    摘要： An active CMOS biosensor chip for fluorescent-based detection is provided that enables time-gated, time-resolved fluorescence spectroscopy. In one embodiment, analytes are loaded with fluorophores that are bound to probe molecules immobilized on the surface of the chip. Photodiodes and other circuitry in the chip are used to measure the fluorescent intensity of the fluorophore at different times. These measurements are then averaged to generate a representation of the transient fluorescent decay response unique to the fluorophores. In addition to its low-cost, compact form, the biosensor chip provides capabilities beyond those of macroscopic instrumentation by enabling time-gated operation for background rejection, easing requirements on optical filters, and by characterizing fluorescence lifetime, allowing for a more detailed characterization of fluorophore labels and their environment. The biosensor chip can be used for a variety of applications including biological, medical, in-the-field applications, and fluorescent lifetime imaging applications.

    摘要翻译： 提供了用于基于荧光的检测的有源CMOS生物传感器芯片，其能够进行时间选择的时间分辨荧光光谱。 在一个实施方案中，分析物载有与固定在芯片表面上的探针分子结合的荧光团。 芯片中的光电二极管和其他电路用于在不同时间测量荧光团的荧光强度。 然后对这些测量进行平均以产生荧光团特有的瞬态荧光衰变响应的表示。 除了其低成本，紧凑的形式之外，生物传感器芯片通过实现背景抑制的时间门控操作，对光学滤波器的放宽要求以及通过表征荧光寿命来提供超出宏观仪器的功能，允许更详细的表征 荧光标签及其环境。 生物传感器芯片可用于各种应用，包括生物，医学，现场应用和荧光寿命成像应用。

    N-(Benzoxazolinyl-2-one)-tricyclic dicarboximides
    20.
    发明授权
    N-(Benzoxazolinyl-2-one)-tricyclic dicarboximides 失效
    N-（苯并恶唑啉-2-酮） - 三环二甲酰亚胺

    公开(公告)号：US4132799A

    公开(公告)日：1979-01-02

    申请号：US831463

    申请日：1977-09-08

    申请人： Kenneth L. Shepard ,  William J. Paleveda, Jr.

    发明人： Kenneth L. Shepard ,  William J. Paleveda, Jr.

    IPC分类号： C07D209/76 ,  A61K31/42 ,  C07D413/04

    CPC分类号： C07D209/76

    摘要： Tricyclicdicarboximides representatively prepared by the Diels-Alder condensation of a cyclic diene such as cycloheptatriene, cyclooctatetraene and the like with a dienophile such as substituted N-phenylmaleimide and the like are disclosed having pharmaceutical utility as minor tranquilizers and anti-convulsants. Also disclosed are processes for the preparation of such tricyclicdicarboximides; pharmaceutical compositions comprising such compounds and their salt, ester and amide derivatives; and methods of treatment comprising administering such compounds and compositions.

    摘要翻译： 公开了通过环己二烯如环庚三烯，环辛四烯等与Dienophile如取代的N-苯基马来酰亚胺等的Diels-Alder缩合反应制备的三环状二甲酰亚胺，其具有作为小镇静剂和抗惊厥药的药用效用。 还公开了制备这种三环二酰亚胺的方法; 包含这些化合物及其盐，酯和酰胺衍生物的药物组合物; 和治疗方法，包括施用这样的化合物和组合物。