公开(公告)号：US20060100788A1

公开(公告)日：2006-05-11

申请号：US11112933

申请日：2005-04-22

申请人： John Carrino ,  Feng Liang ,  Siamak Baharloo ,  Barry Schweitzer

发明人： John Carrino ,  Feng Liang ,  Siamak Baharloo ,  Barry Schweitzer

IPC分类号： G06Q10/00 ,  G06F19/00

CPC分类号： G06Q30/0627 ,  G06Q30/06 ,  G06Q50/22 ,  Y02A90/22 ,  Y02A90/24

摘要： Provided herein are collections of matched biological reagents selected from a larger collection of biological reagents, wherein the collection of matched biological reagents relate to a biological element. Also provided are methods for selling an isolated biomolecule or biological research reagent in a collection of matched biological reagents, and methods for selecting an isolated biomolecule or biological research reagent from a collection of biological reagents.

摘要翻译： 本文提供了选自更大量的生物试剂的匹配生物试剂的集合，其中匹配的生物试剂的收集涉及生物学元件。 还提供了在匹配的生物试剂的集合中销售分离的生物分子或生物研究试剂的方法，以及从生物试剂的集合中选择分离的生物分子或生物研究试剂的方法。

公开(公告)号：US20050136441A1

公开(公告)日：2005-06-23

申请号：US10942565

申请日：2004-09-15

申请人： John Carrino ,  Louis Gerrue ,  Jonathan Diver

发明人： John Carrino ,  Louis Gerrue ,  Jonathan Diver

IPC分类号： C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6837 ,  C12Q1/6844 ,  C40B40/06 ,  C40B60/14 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6844 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00614 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  Y10T436/143333 ,  C12Q2531/113 ,  C12Q2535/125 ,  C12Q2565/501

摘要： This invention relates to methods for detecting the extent of hybridization of a nucleic acid in a sample to a probe nucleic acid sequence by electronically hybridizing the nucleic acid in the sample to the probe, utilizing the hybridized nucleic acid as a template in a nucleic acid polymerase reaction to extend the bound probe and incorporate a labeled nucleotide, and detecting the labeled product. This invention also relates to methods of detecting the extent of hybridization of a plurality of nucleic acids in a sample to a plurality of nucleic acid probes using similar methods.

摘要翻译： 本发明涉及通过使用杂交核酸作为模板在核酸聚合酶中将样品中的核酸与探针电子杂交来检测样品中的核酸与探针核酸序列的杂交程度的方法 扩增结合探针并掺入标记核苷酸并检测标记产物的反应。 本发明还涉及使用类似方法检测样品中多种核酸与多种核酸探针的杂交程度的方法。

公开(公告)号：US20130004996A1

公开(公告)日：2013-01-03

申请号：US13219440

申请日：2011-08-26

申请人： Jonathan D. CHESNUT ,  John Carrino ,  Louis Leong ,  Knut Madden ,  Martin A. G. Gleeson ,  James Fan ,  Michael A. Brasch ,  David Cheo ,  James L. Hartley ,  Devon R.N. Byrd ,  Gary F. Temple

发明人： Jonathan D. CHESNUT ,  John Carrino ,  Louis Leong ,  Knut Madden ,  Martin A. G. Gleeson ,  James Fan ,  Michael A. Brasch ,  David Cheo ,  James L. Hartley ,  Devon R.N. Byrd ,  Gary F. Temple

IPC分类号： C12P19/34 ,  C12N9/96 ,  C12N15/66 ,  C07H21/04

CPC分类号： C12N15/66 ,  C12N9/90 ,  C12N15/10 ,  C12N15/64 ,  C12N15/902 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/70 ,  C12N2840/20 ,  C12P19/34 ,  C12Y599/01

摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according tb the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.

摘要翻译： 本发明提供了用于重组克隆的组合物和方法。 组合物包括具有多个重组位点和/或多个拓扑异构酶识别位点的载体。 该方法允许同时克隆两种或更多种不同的核酸分子。 在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。 本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。 本发明还提供包含本发明的核酸分子或根据本发明的方法制备的宿主细胞，并且还提供了包含本发明的组合物，宿主细胞和核酸分子的试剂盒，其可用于合成核酸分子 根据本发明的方法。

公开(公告)号：US08207318B2

公开(公告)日：2012-06-26

申请号：US12112649

申请日：2008-04-30

申请人： James Fan ,  John Carrino ,  Jonathan Chesnut ,  Knut Madden ,  Martin Gleeson ,  Robert Bennett

发明人： James Fan ,  John Carrino ,  Jonathan Chesnut ,  Knut Madden ,  Martin Gleeson ,  Robert Bennett

IPC分类号： C07H21/02 ,  C12Q1/68

CPC分类号： C12N15/10 ,  C12N9/90 ,  C12N15/111 ,  C12N15/64 ,  C12N15/66 ,  C12N2310/14 ,  C12N2330/30 ,  C12P19/34 ,  C12Q1/6855 ,  C12Y599/01002 ,  C12Q2521/519

摘要： A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.

摘要翻译： 通过使两个或多个ds核苷酸序列与拓扑异构酶接触的条件使得在第一个ds核苷酸序列的至少一个末端的两个末端共价连接的情况下，产生双链（ds）重组核酸分子共价连接的双链（ds）重组核酸分子的方法 提供了拓扑异构酶至第二个ds核苷酸序列的至少一个末端的两个末端。 还提供了一种用于产生共价连接在一条链中的ds重组核酸分子的方法，其通过使两个或更多个ds核苷酸序列与IA型拓扑异构酶接触，使得一个或两个末端的一条链但不是两条链 第一个ds核苷酸序列由拓扑异构酶共价连接。 还提供了用于进行这些方法的组合物，以及由这些方法产生的组合物，以及包含用于方便地实施该方法的组分的试剂盒。

公开(公告)号：US20070216535A1

公开(公告)日：2007-09-20

申请号：US11374820

申请日：2006-03-14

申请人： John Carrino

发明人： John Carrino

IPC分类号： G08B23/00 ,  G08B29/00 ,  G08B1/00

CPC分类号： G08B27/00 ,  G06F17/3087 ,  G06Q10/10 ,  G08B25/14

摘要： A versatile Citizen Alert System is connected to Internet and private databases to acquire real time information useful for subscribed users. During registration, users are required to provide multimedia contact information, including telephone numbers, cell phone numbers, pager information, Blackberry or PDA information, e-mail address, and physical address, along with user defined sensitivities to alerts, including sensitivities to natural emergencies, man made emergencies and professional requirements. The system has a local or remote administrator that constantly analyzes the acquired data in accordance with requirements of each and every user, orders the data to generate alert information specific to a user, and communicates the alert to each and every user using multiple delivery platforms. A user specific, secured web page providing details of alert and hyperlinks constitutes an additional information source.

摘要翻译： 多功能公民警报系统连接到互联网和私人数据库，以获取对订阅用户有用的实时信息。 在注册期间，用户需要提供多媒体联系信息，包括电话号码，手机号码，寻呼机信息，黑莓或PDA信息，电子邮件地址和物理地址，以及用户定义的敏感信息，包括对自然紧急情况的敏感性 ，人为紧急情况和专业要求。 该系统具有本地或远程管理员，其根据每个用户的要求不断分析所获取的数据，订购数据以生成特定于用户的警报信息，并且使用多个传送平台将警报传达给每个用户。 提供警报和超链接详细信息的用户特定的安全网页构成了附加信息源。

公开(公告)号：US07244560B2

公开(公告)日：2007-07-17

申请号：US10005876

申请日：2001-12-07

申请人： Jonathan D. Chestnut ,  John Carrino ,  Louis Leong ,  Knut Madden ,  Martin Gleeson ,  James Fan ,  Michael A. Brasch ,  David Cheo ,  James L. Hartley ,  Devon R.N. Byrd ,  Gary F. Temple

发明人： Jonathan D. Chestnut ,  John Carrino ,  Louis Leong ,  Knut Madden ,  Martin Gleeson ,  James Fan ,  Michael A. Brasch ,  David Cheo ,  James L. Hartley ,  Devon R.N. Byrd ,  Gary F. Temple

IPC分类号： C12Q1/68

CPC分类号： C12N15/66 ,  C12N9/90 ,  C12N15/10 ,  C12N15/64

摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.

公开(公告)号：US07033801B2

公开(公告)日：2006-04-25

申请号：US10014128

申请日：2001-12-07

申请人： John Carrino ,  James Fan ,  Robert P. Bennett ,  Jonathan D. Chesnut ,  Martin A. Gleeson ,  Knut R. Madden

发明人： John Carrino ,  James Fan ,  Robert P. Bennett ,  Jonathan D. Chesnut ,  Martin A. Gleeson ,  Knut R. Madden

IPC分类号： C12P19/34

CPC分类号： C12P19/34 ,  C12N9/90 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/6855 ,  C12Q2521/519

摘要： A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.

摘要翻译： 通过使两个或多个ds核苷酸序列与拓扑异构酶接触的条件使得在第一个ds核苷酸序列的至少一个末端的两个末端共价连接的情况下，产生在两条链中共价连接的双链（ds）重组核酸分子的方法 提供了拓扑异构酶至第二个ds核苷酸序列的至少一个末端的两个末端。 还提供了一种用于产生共价连接在一条链中的ds重组核酸分子的方法，其通过使两个或更多个ds核苷酸序列与IA型拓扑异构酶接触，使得一个或两个末端的一条链但不是两条链 第一个ds核苷酸序列由拓扑异构酶共价连接。 还提供了用于进行这些方法的组合物，以及由这些方法产生的组合物，以及包含用于方便地实施该方法的组分的试剂盒。

公开(公告)号：US20060029935A1

公开(公告)日：2006-02-09

申请号：US10014128

申请日：2001-12-07

申请人： John Carrino ,  James Fan ,  Robert Bennett ,  Jonathan Chesnut ,  Martin Gleeson ,  Knut Madden

发明人： John Carrino ,  James Fan ,  Robert Bennett ,  Jonathan Chesnut ,  Martin Gleeson ,  Knut Madden

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12P19/34 ,  C12N9/90 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/6855 ,  C12Q2521/519

摘要： A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.

摘要翻译： 通过使两个或多个ds核苷酸序列与拓扑异构酶接触的条件使得在第一个ds核苷酸序列的至少一个末端的两个末端共价连接的情况下，产生双链（ds）重组核酸分子共价连接的双链（ds）重组核酸分子的方法 提供了拓扑异构酶至第二个ds核苷酸序列的至少一个末端的两个末端。 还提供了一种用于产生共价连接在一条链中的ds重组核酸分子的方法，其通过使两个或更多个ds核苷酸序列与IA型拓扑异构酶接触，使得一个或两个末端的一条链但不是两条链 第一个ds核苷酸序列由拓扑异构酶共价连接。 还提供了用于进行这些方法的组合物，以及由这些方法产生的组合物，以及包含用于方便地实施该方法的组分的试剂盒。

公开(公告)号：US20060008817A1

公开(公告)日：2006-01-12

申请号：US10991803

申请日：2004-11-17

申请人： John Carrino ,  James Fan ,  Robert Bennett ,  Jonathan Chesnut ,  Martin Gleeson ,  Knut Madden

发明人： John Carrino ,  James Fan ,  Robert Bennett ,  Jonathan Chesnut ,  Martin Gleeson ,  Knut Madden

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12N15/10 ,  C12N9/90 ,  C12N15/111 ,  C12N15/64 ,  C12N15/66 ,  C12N2310/14 ,  C12N2330/30 ,  C12P19/34 ,  C12Q1/6855 ,  C12Y599/01002 ,  C12Q2521/519

摘要： A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.