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    METHODS AND COMPOSITIONS FOR DIFFERENTIAL EXPANSION OF FETAL CELLS IN MATERNAL BLOOD AND THEIR USE
    2.
    发明申请
    METHODS AND COMPOSITIONS FOR DIFFERENTIAL EXPANSION OF FETAL CELLS IN MATERNAL BLOOD AND THEIR USE 审中-公开
    方法和组合物在宫颈细胞中的差异扩大及其使用

    公开(公告)号：US20110039258A1

    公开(公告)日：2011-02-17

    申请号：US12445732

    申请日：2007-10-15

    申请人： Patricia McNeeley ,  Philippe Marchand ,  Jonathan Diver

    发明人： Patricia McNeeley ,  Philippe Marchand ,  Jonathan Diver

    IPC分类号： C12Q1/68 ,  C12N5/02 ,  C12Q1/02

    CPC分类号： C12N5/0634 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/125 ,  C12N2501/23

    摘要： Disclosed is a method and compositions for the differential expansion of fetal cells over maternal cells. In the method, cells from a sample of maternal blood containing CD34+ cells of both maternal and fetal origin are incubated in the presence of Stem Cell Factor in serum free media. It has been discovered that incubation of fetal cells in the presence of SCF will preferentially expand the fetal cells relative to adult cells. Fetal cells can also be identified, enriched or obtained by differential expansion of the fetal cells during colony formation. It has been discovered that differential expansion of fetal cells can result in colonies of fetal cells that are larger than colonies of adult cells. The fetal CD34+ cells can be expanded without generation of significant clonal genetic artifacts during expansion. Also disclosed is a method and compositions for producing differentiated fetal cells. It has been discovered that differentiated fetal cells have markers that distinguish the fetal cells from adult cells. Also disclosed are fetal cells made or obtained using the disclosed methods. For example, disclosed are expanded and/or differentiated fetal cells. The disclosed fetal cells can be used for any purpose and in any way that fetal cells can be used. The disclosed fetal cells are particularly useful for prenatal analysis of a gestating fetus.

    摘要翻译： 公开了胎儿细胞在母细胞上差异扩增的方法和组合物。 在该方法中，含有母体和胎儿来源的含有CD34 +细胞的母体血液样品的细胞在无血清培养基中的干细胞因子存在下孵育。 已经发现，在SCF存在下胎儿细胞的孵育将相对于成体细胞优先扩张胎儿细胞。 胎儿细胞也可以在菌落形成期间通过胎儿细胞的差异扩增来鉴定，富集或获得。 已经发现，胎儿细胞的差异扩增可导致比成体细胞的集落大的胎儿细胞的集落。 胎儿CD34 +细胞可以在扩增期间扩增而不产生显着的克隆遗传伪像。 还公开了用于产生分化的胎儿细胞的方法和组合物。 已经发现，分化的胎儿细胞具有区分胎儿细胞和成体细胞的标记。 还公开了使用所公开的方法制造或获得的胎儿细胞。 例如，所公开的是扩增和/或分化的胎儿细胞。 所公开的胎儿细胞可以用于任何目的，并且可以以任何方式使用胎儿细胞。 所公开的胎儿细胞特别可用于孕胎分娩。