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    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same
    11.
    发明申请
    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same 审中-公开

    公开(公告)号：US20190040427A1

    公开(公告)日：2019-02-07

    申请号：US15936440

    申请日：2018-03-27

    Applicant: Kiverdi, Inc.

    Inventor： Itzhak Kurek ,  John S. Reed ,  Lisa Dyson ,  Henrik Fyrst ,  Christer Jansson ,  David Galgoczy

    IPC: C12P7/64 ,  C12P5/00 ,  C12N1/20 ,  C12P7/04 ,  C12P5/02 ,  C12N9/88 ,  C12N9/16 ,  C12N9/02

    Abstract: Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same
    12.
    发明申请
    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same 审中-公开

    公开(公告)号：US20170152533A1

    公开(公告)日：2017-06-01

    申请号：US15233512

    申请日：2016-08-10

    Applicant: Kiverdi, Inc.

    Inventor： Itzhak Kurek ,  John S. Reed ,  Lisa Dyson ,  Henrik Fyrst ,  Christer Jansson ,  David Galgoczy

    IPC: C12P7/64 ,  C12N9/02 ,  C12N9/16

    CPC classification number: C12P7/649 ,  C12N1/20 ,  C12N9/0008 ,  C12N9/0083 ,  C12N9/16 ,  C12N9/88 ,  C12P5/002 ,  C12P5/026 ,  C12P7/04 ,  C12P7/6409 ,  C12Y114/99033 ,  C12Y301/02014 ,  Y02E50/13 ,  Y02P20/52

    Abstract: Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

    Use of oxyhydrogen microorganisms for non-photosynthetic carbon capture and conversion of inorganic and/or C1 carbon sources into useful organic compounds
    19.
    发明授权
    Use of oxyhydrogen microorganisms for non-photosynthetic carbon capture and conversion of inorganic and/or C1 carbon sources into useful organic compounds 有权
    使用氢氧化物微生物进行非光合碳捕获，并将无机和/或C1碳源转化为有用的有机化合物

    公开(公告)号：US09085785B2

    公开(公告)日：2015-07-21

    申请号：US14033013

    申请日：2013-09-20

    Applicant: Kiverdi, Inc.

    Inventor： John S. Reed ,  Lisa Dyson

    IPC: C12M1/00 ,  C25B15/02 ,  C12P7/62 ,  C12N1/12 ,  C12N1/20 ,  C25B1/04

    CPC classification number: C12P7/625 ,  C12M23/34 ,  C12M29/02 ,  C12M29/08 ,  C12M29/18 ,  C12M29/20 ,  C12M43/04 ,  C12M47/02 ,  C12N1/12 ,  C12N1/20 ,  C25B1/04 ,  C25B15/02 ,  Y02E60/366 ,  Y02P20/133

    Abstract: Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or C1 carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing C1 chemicals including but not limited to various syngas compositions, into organic chemicals including biofuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or C1 carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO2 in one or more steps of the process.

    Abstract translation: 捕获并转化二氧化碳和/或其他形式的无机碳和/或C1碳源的混合生物和化学过程的组合物和方法，包括但不限于一氧化碳，甲烷，甲醇，甲酸盐或甲酸，和/ 或包含C1化学品（包括但不限于各种合成气组合物）的混合物，包括生物燃料或其他有价值的生物质，化学，工业或医药产品的有机化学品。 在某些实施方案中，本发明通过在该方法的一个或多个步骤中利用能够进行氢氧化反应和CO 2的自养固定的微生物将无机碳或C1碳源固定在较长的碳链有机化学品中。

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