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公开(公告)号：US11756196B2
公开(公告)日：2023-09-12
申请号：US17143281
申请日：2021-01-07
Applicant: Leica Microsystems CMS GmbH
Inventor： Falk Schlaudraff , Christoph Greb
CPC classification number: G06T7/0014 , G06T2207/10056 , G06T2207/10148 , G06T2207/30024
Abstract: A method for checking a dissection process in a laser microdissection system includes carrying out the dissection process for cutting out a dissectate from an object in a first region of the object by a laser beam. First image data is acquired of at least the first region of the object after the dissection process. It is examined whether the first image data has sharp structures within a region to be separated by the dissection process in order to determine whether the dissection process was successful.
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12.发明授权公开(公告)号：US09804144B2
公开(公告)日：2017-10-31
申请号：US14049241
申请日：2013-10-09
Applicant: Leica Microsystems CMS GmbH
Inventor： Falk Schlaudraff , Florian Hoffmann
IPC: G01N33/483 , G01N1/28
CPC classification number: G01N33/483 , G01N1/2813 , G01N2001/282 , G01N2001/2886
Abstract: A method for laser microdissection of a laser microdissection region of a prepared specimen includes driving a holder for the specimen into a holding position using a control device. First and second digital images are captured that depict a same portion of the prepared specimen, with the first image depicting the portion under at least one first microscopic examination method and the second image depicting the portion under at least a second microscopic examination method. A live overlay image is generated of the portion of the prepared image in a live mode. The live overlay is presented on a display area with the images overlaid onto one another. A marking is generated and captured on the live overlay image so as to define the laser microdissection region.
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13.发明申请公开(公告)号：US20160202150A1
公开(公告)日：2016-07-14
申请号：US14914014
申请日：2014-08-19
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Falk Schlaudraff
IPC: G01N1/06
CPC classification number: G01N1/06 , G01N1/286 , G01N2001/045 , G01N2001/2886
Abstract: A laser microdissection system includes a microscope that includes a reflected light device having a laser deflector which guides a laser beam provided by a laser through a microscope objective of the microscope onto a sample region for receiving a biological sample and which moves a point of impingement of the laser beam in the sample region. The laser microdissection system also includes a rinsing device comprising fluid dispenser configured to provide a suspension fluid in the sample region and a fluid remover arranged on the rinsing device configured to remove a suspension produced using the suspension fluid from the sample region.
Abstract translation: 激光显微切割系统包括显微镜，其包括具有激光偏转器的反射光装置，所述激光偏转器将由激光提供的激光束通过显微镜的显微镜物镜引导到用于接收生物样品的样品区域上，并且移动点 激光束在样品区域。 激光显微切割系统还包括漂洗装置，其包括被配置为在样品区域中提供悬浮液体的流体分配器和布置在漂洗装置上的流体去除器，其构造成去除使用来自样品区域的悬浮液产生的悬浮液。
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公开(公告)号：US20130307395A1
公开(公告)日：2013-11-21
申请号：US13892656
申请日：2013-05-13
Applicant: Leica Microsystems CMS GmbH
Inventor： Falk Schlaudraff
IPC: A47B88/04
CPC classification number: B01L9/52 , A61B10/0096 , B01L2200/025 , B01L2200/028 , B01L2300/0822 , G01N2001/284 , G01N2035/00089 , G02B21/34
Abstract: The invention relates to a slide storage assembly (300) having spacer elements (100, 200) and a slide container (301), which is embodied to accommodate a stack of at least two slide layers, each slide layer comprising a slide (400) and at least two of the spacer elements (100, 200).
Abstract translation: 本发明涉及具有间隔元件（100,200）和滑动容器（301）的滑动存储组件（300），其被实施为容纳至少两个滑动层的堆叠，每个滑动层包括滑块（400） 和至少两个间隔元件（100,200）。
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公开(公告)号：US12106454B2
公开(公告)日：2024-10-01
申请号：US17637480
申请日：2020-08-31
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Falk Schlaudraff , Markus Schechter , Frank Sieckmann , Oliver Schlicker
IPC: G06T5/73 , G02B7/38 , G02B21/12 , G02B21/24 , G06F17/13 , G06F17/15 , G06T5/10 , G06T5/20 , G06T5/70 , G06T7/00 , G06V10/30 , H04L25/02 , H04N25/615
CPC classification number: G06T5/73 , G02B7/38 , G02B21/12 , G02B21/244 , G06F17/13 , G06F17/15 , G06T5/10 , G06T5/20 , G06T5/70 , G06T7/0012 , G06V10/30 , H04L25/025 , H04N25/615 , G06T2207/10056 , G06T2207/10068 , G06T2207/20056 , G06T2207/20081 , G06T2207/20084 , G06T2207/30024
Abstract: An image processing apparatus for determining a focused output image in a passive autofocus system is configured to retrieve a set of input images and compute a baseline estimate for at least one input image. The baseline estimate represents image structures in the input image. The image structures have a length scale larger than a predetermined image feature length scale. The image processing apparatus is further configured to compute a set of output images, wherein each output image of the set of output images is computed based on one of a different input image of the set of input images and the at least one baseline estimate for the different input image and the at least one baseline estimate for a respective different input image. The image processing apparatus is further configured to determine one output image of the set of output images as the focused output image.
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Patent Agency Ranking
公开(公告)号：US11874207B2
公开(公告)日：2024-01-16
申请号：US17424521
申请日：2020-01-23
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Florian Hoffmann , Falk Schlaudraff
CPC classification number: G01N1/2813 , G01N1/286 , G02B19/0047 , G01N2001/284 , G01N2001/2886
Abstract: A method for laser microdissection includes: processing a microscopic examination object by a laser beam using tuples of coordinate values which respectively indicate positions of target points on the examination object at least in a first spatial direction and a second spatial direction orthogonal to the first spatial direction, positions of at least three reference points being ascertained beforehand in each case in the first and second spatial directions and also in a third spatial direction orthogonal to the first and second spatial directions; defining a reference plane based on the positions of the reference points; and determining, for the target points, further coordinate values indicating an expected position of the target points on the examination object in the third spatial direction in each case, as determined further coordinate values, the determining of the further coordinate values being performed depending on the defined reference plane.
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公开(公告)号：US20220276136A1
公开(公告)日：2022-09-01
申请号：US17667580
申请日：2022-02-09
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Falk Schlaudraff , Florian Hoffmann , Christoph Greb
Abstract: A method for obtaining dissectates from a microscopic sample using a laser microdissection system having a laser unit includes: a) at least partially circumcising and releasing from the sample dissectate regions of the sample as the dissectates using laser pulses provided by the laser unit; b) transferring the dissectates, by being released from the sample, along dissectate trajectories into receptacles of a dissectate collection unit; and c) positioning the receptacles of a dissectate collection unit using a positioning unit to collect the dissectates. The positioning of the receptacles of the dissectate collection unit using the positioning unit is automatically performed based on estimates of the dissectate trajectories, the estimates of the dissectate trajectories being obtained in a learning mode, the learning mode including obtaining dissectates by repeatedly performing at least steps a) and b). Parameters of the dissectate trajectories are determined for a plurality of dissectates.
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公开(公告)号：US20210404965A1
公开(公告)日：2021-12-30
申请号：US17352401
申请日：2021-06-21
Applicant: Leica Microsystems CMS GmbH
Inventor： Nariman Ansari , Falk Schlaudraff
IPC: G01N21/64
Abstract: A method for optically examining a biological sample includes: preparing a light-activatable specimen including the biological sample; activating a target region of the light-activatable specimen by irradiating activation light into the light-activatable specimen, the activation light being formed by a light sheet that illuminates a plane in the light-activatable specimen, the plane including at least a part of the biological sample; and imaging the plane illuminated with the light sheet.
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公开(公告)号：US11079583B2
公开(公告)日：2021-08-03
申请号：US16310835
申请日：2017-06-30
Applicant: Leica Microsystems CMS GmbH
Inventor： Falk Schlaudraff
Abstract: A laser microscope system includes a microscope and a lens defining an optical axis and a rear focal plane, as well as a laser and an optical laser system coupling a laser beam into the microscope such that it passes through the rear focal plane of the lens at a fixed point. The optical laser system has an offset lens movable along an axis of the laser beam path to move the laser beam focus in the direction of the optical axis. The optical laser system has a compensating lens arranged in the laser beam path and movable along the axis of the laser beam path. A controller and/or a mechanical coupling device carries out a movement of the compensating lens along the axis of the laser beam path when the lens is moved such that the laser beam continues to pass through the fixed point.
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公开(公告)号：US20180149561A1
公开(公告)日：2018-05-31
申请号：US15574492
申请日：2016-05-20
Applicant: Leica Microsystems CMS GmbH
Inventor： Falk Schlaudraff , Blagovesta Wegner
CPC classification number: G01N1/286 , G01N1/2813 , G01N2001/282 , G01N2001/2886 , G02B21/32 , G02B21/34 , G02B21/365 , G06T7/73 , G06T2207/10056 , G06T2207/30204
Abstract: A method for examining and processing a microscopic sample arranged on a slide includes producing reference markings on the slide by a laser beam of a laser microdissection system. A digital image of the sample and the reference markings on the slide is produced by a digital optical imaging device. An image region is defined and first position information data which indicate a position of the image region is generated. The reference markings are identified in the image and second position information data which indicate a position of the reference markings in the image is generated. The reference markings are identified, and third position information data which indicate the position of the reference markings in the laser microdissection system is generated. The first, second and third position information data are correlated and a sample region which corresponds to the image region is processed.
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