公开(公告)号：US20160258011A1

公开(公告)日：2016-09-08

申请号：US15061273

申请日：2016-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Phil WAGGONER ,  James A. BALL ,  Wolfgang HINZ ,  Michael L. MINTO ,  Scott PARKER ,  David M. COX ,  Alexander MASTROIANNI ,  Jeremy GRAY ,  Marc GLAZER ,  Kimberly GORRELL

IPC: C12Q1/68 ,  G01N27/414

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Abstract translation: 传感器装置包括基板，设置在基板上的半导体器件，具有表面电极结构的半导体器件和形成在表面电极结构上的糖类涂层。 在使用前可以除去糖衣。 该半导体器件还可以包括一个阱以及可选地布置在阱中的珠。

公开(公告)号：US20160003768A1

公开(公告)日：2016-01-07

申请号：US14789795

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald L. CICERO ,  Marc GLAZER ,  Yufang WANG ,  Christina E. INMAN ,  Jeremy GRAY ,  Joseph KOSCINSKI ,  James A. BALL ,  Phil WAGGONER ,  Alexander MASTROIANNI

IPC: G01N27/414

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Abstract translation: 传感器部件包括传感器，传感器包括与传感器协作的传感器表面和反应部位，并暴露传感器表面。 反应位点包括反应位点表面。 表面剂与反应位点表面或传感器表面结合。 表面活性剂包括在反应位点表面或传感器表面上与布朗斯台德碱或路易斯酸官能团反应的表面活性官能团，并且包括不具有供体电子对的远端官能团。

公开(公告)号：US20160001249A1

公开(公告)日：2016-01-07

申请号：US14789650

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Ronald L. CICERO ,  Yufang WANG ,  Alexander MASTROIANNI ,  Prasanna Krishnan THWAR ,  Jeremy GRAY ,  Marc GLAZER ,  Tommie Lloyd LINCECUM, JR. ,  Mohammad ALANJARY ,  Joseph KOSCINSKI

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00677 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2523/32 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/155 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.

Abstract translation: 在传感器基板上加载珠粒的方法包括将包含珠粒的悬浮液施加到在传感器基底上限定的流动池中。 传感器基板包括多个孔。 珠粒至少部分沉积到多个孔中。 该方法还包括从流动池中去除液体，从流动池中蒸发液体，例如通过抽吸空气流过流动池; 以及将水合溶液施加到所述流动池。

公开(公告)号：US20140191292A1

公开(公告)日：2014-07-10

申请号：US13734696

申请日：2013-01-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christina INMAN ,  Alexander MASTROIANNI ,  Wolfgang HINZ ,  Shifeng LI ,  Scott BENSON

IPC: H01L27/105 ,  H01L21/8234

CPC classification number: H01L21/31105 ,  G01N27/4145 ,  H01L21/31144

Abstract: A method of manufacturing a sensor, the method including forming an array of chemically-sensitive field effect transistors (chemFETs), depositing a dielectric layer over the chemFETs in the array, depositing a protective layer over the dielectric layer, etching the dielectric layer and the protective layer to form cavities corresponding to sensing surfaces of the chemFETs, and removing the protective layer. The method further includes, etching the dielectric layer and the protective layer together to form cavities corresponding to sensing surfaces of the chemFETs. The protective layer is at least one of a polymer, photoresist material, noble metal, copper oxide, and zinc oxide. The protective layer is removed using at least one of sodium hydroxide, organic solvent, aqua regia, ammonium carbonate, hydrochloric acid, acetic acid, and phosphoric acid.

Abstract translation: 一种制造传感器的方法，所述方法包括形成化学敏感场效应晶体管阵列（chemFET），在阵列中的chemFET上沉积电介质层，在电介质层上沉积保护层，蚀刻电介质层和 保护层以形成对应于chemFET的感测表面的空腔，以及去除保护层。 该方法还包括：将电介质层和保护层一起蚀刻以形成对应于chemFET的感测表面的空腔。 保护层是聚合物，光致抗蚀剂材料，贵金属，氧化铜和氧化锌中的至少一种。 使用氢氧化钠，有机溶剂，王水，碳酸铵，盐酸，乙酸和磷酸中的至少一种除去保护层。